Results 11 to 20 of about 59,552 (223)

A Hairpin Ribozyme Derived Spliceozyme

ChemBioChem, 2023
The vast majority of RNA splicing in today‘s organisms is achieved by the highly regulated and precise removal of introns from pre‐mRNAs via the spliceosome. Here we present a model of how RNA splicing may have occurred in earlier life forms.
Jikang Zhu, R. Hieronymus, Sabine Müller   +2 more
semanticscholar   +3 more sources

Hydrostatic and osmotic pressure study of the hairpin ribozyme [PDF]

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2006
The recent discovery of numerous catalytically active RNAs in various living species as well as the in vitro selection of a large series of RNA aptamers able to bind specifically various molecules such as metabolites and co-factors, emphasize the adaptability of RNAs through the plasticity of their secondary structure.
Guy Hervé, Sylvia Tobé, Thomas Heams, Jacques Vergne, Marie‐Christine Maurel   +4 more
core   +10 more sources

A split ribozyme system for in vivo plant RNA imaging and genetic engineering. [PDF]

Plant Biotechnol J
Summary RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops
Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X.   +11 more
europepmc   +2 more sources

Site-Selective Modification and Labeling of Native RNA. [PDF]

Chemistry
The review highlights recent advancements in site‐selective modification and labeling of native RNA, an important area for the development of RNA therapeutics and fundamental RNA research. The applications, current challenges of existing methods and potential future direction of this field are also discussed.
Kha TK, Zhao Y, Zhu RY.
europepmc   +2 more sources

The pH dependence of hairpin ribozyme catalysis reflects ionization of an active site adenine. [PDF]

J Biol Chem, 2011
Understanding how self-cleaving ribozymes mediate catalysis is crucial in light of compelling evidence that human and bacterial gene expression can be regulated through RNA self-cleavage.
Cottrell JW, Scott LG, Fedor MJ.
europepmc   +2 more sources

Catalytic importance of a protonated adenosine in the hairpin ribozyme active site. [PDF]

Biochemistry, 2010
The hairpin ribozyme accelerates the rate of phosphodiester transfer reactions by at least 5 orders of magnitude. To achieve this rate enhancement, the active site forms via a substrate helix docking event that constrains the scissile phosphate linkage ...
Suydam IT, Levandoski SD, Strobel SA.
europepmc   +2 more sources

Selective Nonenzymatic Formation of Biologically Common RNA Hairpins. [PDF]

Angew Chem Int Ed Engl
Template‐free RNA loop‐closing ligation selectively generates RNA tetraloops that are prevalent in biology, as demonstrated by high‐throughput sequencing. Utilizing high‐yield overhang sequences enables the efficient assembly of functional RNAs. Abstract The prebiotic formation of RNA building blocks is well‐supported experimentally, yet the emergence ...
Wu LF, Zhang J, Cornwell-Arquitt R, Hendrix DA, Radakovic A, Szostak JW.   +5 more
europepmc   +2 more sources

Computational De Novo Design of Group II Introns Yields Highly Active Ribozymes. [PDF]

Chembiochem
Group II Introns (G2Is) are large self‐splicing ribozymes with promising biotechnological applications. This study utilized RNA inverse folding to design three novel G2Is. The designed intron Arq.I2, an unexpectedly proficient ribozyme, self‐splices not only in vitro, but also in Escherichia coli cells.
Szokoli D, Nwosu NE, Glatt LM, Mutschler H.   +3 more
europepmc   +2 more sources

QM/MM studies of hairpin ribozyme self-cleavage suggest the feasibility of multiple competing reaction mechanisms. [PDF]

J Phys Chem B, 2011
The hairpin ribozyme is a prominent member of small ribozymes since it does not require metal ions to achieve catalysis. Guanine 8 (G8) and adenine 38 (A38) have been identified as key participants in self-cleavage and -ligation.
Mlýnský V, Banáš P, Walter NG, Šponer J, Otyepka M.   +4 more
europepmc   +2 more sources

Mutagenesis of the hairpin ribozyme [PDF]

Nucleic Acids Research, 1994
Extensive in vitro mutagenesis studies have been performed on the hairpin ribozyme and substrate in an effort to refine the overall secondary structure of the molecule and provide further insight into what elements are essential for activity. A secondary structure consisting of four helices and five loop regions remains the basic model as originally ...
Pamela J. Anderson, Joseph Monforte, Richard Tritz, Steven Nesbitt, John E. Hearst, Arnold Hamper   +5 more
openalex   +5 more sources