Screening for Important Base Identities in the Hairpin Ribozyme by In Vitro Selection for Cleavage
BioTechniques, 1998 Random mutagenesis followed by an in vitro selection procedure was shown to be capable of identifying important bases of the hairpin ribozyme for cleavage of an RNA target sequence.
Andrew Siwkowski +3 more
doaj +2 more sources
Hydrostatic and osmotic pressure study of the hairpin ribozyme. [PDF]
, 2006 The recent discovery of numerous catalytically active RNAs in various living species as well as the in vitro selection of a large series of RNA aptamers able to bind specifically various molecules such as metabolites and co-factors, emphasize the ...
Heams, Thomas +4 more
core +5 more sources
Functional Analysis of Hairpin Ribozyme Active Site Architecture [PDF]
Journal of Biological Chemistry, 2007 The hairpin ribozyme is a small catalytic motif found in plant satellite RNAs where it catalyzes a reversible self-cleavage reaction during processing of replication intermediates. Crystallographic studies of hairpin ribozymes have provided high resolution views of the RNA functional groups that comprise the active site and stimulated biochemical ...
Joseph W, Cottrell +2 more
openaire +4 more sources
A Conserved Target Site in HIV-1 Gag RNA is Accessible to Inhibition by Both an HDV Ribozyme and a Short Hairpin RNA [PDF]
Molecular Therapy: Nucleic Acids, 2014 Antisense-based molecules targeting HIV-1 RNA have the potential to be used as part of gene or drug therapy to treat HIV-1 infection. In this study, HIV-1 RNA was screened to identify more conserved and accessible target sites for ribozymes based on the ...
Robert J Scarborough +7 more
doaj +2 more sources
Thermodynamics and kinetics of RNA tertiary structure formation in the junctionless hairpin ribozyme. [PDF]
Biophys Chem, 2017 White NA, Hoogstraten CG.
europepmc +3 more sources
Kinetic Mechanism of the Hairpin Ribozyme [PDF]
Journal of Biological Chemistry, 1997 To investigate the relationship between RNA folding and ribozyme catalysis, we have carried out a detailed kinetic analysis of four structural derivatives of the hairpin ribozyme. Optimal and suboptimal (wild-type) substrate sequences were studied in conjunction with stabilization of helix 4, which supports formation of the
Esteban, José A. +2 more
openaire +4 more sources
Intermolecular domain docking in the hairpin ribozyme: metal dependence, binding kinetics and catalysis. [PDF]
RNA Biol, 2013 Sumita M +3 more
europepmc +3 more sources
Evidence for the role of active site residues in the hairpin ribozyme from molecular simulations along the reaction path. [PDF]
J Am Chem Soc, 2014 Heldenbrand H +5 more
europepmc +3 more sources
The pH dependence of hairpin ribozyme catalysis reflects ionization of an active site adenine. [PDF]
J Biol Chem, 2011 Cottrell JW, Scott LG, Fedor MJ.
europepmc +3 more sources
The RNA-Puzzles Assessments of RNA-Only Targets in CASP16. [PDF]
ProteinsABSTRACT RNA‐Puzzles was launched in 2011 as a collaborative effort dedicated to advancing and improving RNA 3D structure prediction. The automatic evaluation protocols for comparisons between prediction and experiment developed within RNA‐Puzzles are applied to the 2024 CASP16 competition.
Westhof E, Sun H, Bu F, Miao Z.
europepmc +2 more sources