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Folding and catalysis of the hairpin ribozyme
Biochemical Society Transactions, 2005The active form of the hairpin ribozyme is brought about by the interaction of two formally unpaired loops. In a natural molecule, these are present on two adjacent arms of a four-way junction. Although activity can be obtained in molecules lacking this junction, the junction is important in the promotion of the folded state of the ribozyme under ...
Timothy J. Wilson +3 more
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Biopolymers, 2003
AbstractThe hairpin ribozyme is a naturally occurring RNA that catalyzes sequence‐specific cleavage and ligation of RNA. It has been the subject of extensive biochemical and structural studies, perhaps the most detailed for any catalytic RNA to date. Comparison of the structures of its constituent domains free and fully assembled demonstrates that the ...
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AbstractThe hairpin ribozyme is a naturally occurring RNA that catalyzes sequence‐specific cleavage and ligation of RNA. It has been the subject of extensive biochemical and structural studies, perhaps the most detailed for any catalytic RNA to date. Comparison of the structures of its constituent domains free and fully assembled demonstrates that the ...
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Characterization of Hairpin Ribozyme Reactions
2013Hairpin ribozymes are small RNA catalytic motifs naturally found in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV), and arabis mosaic virus (ArMV). The catalytic activity of the hairpin ribozyme extends to both cleavage and ligation reactions.
Preeti Bajaj, Christian Hammann
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Structure and function of the hairpin ribozyme
Journal of Molecular Biology, 2000The hairpin ribozyme belongs to the family of small catalytic RNAs that cleave RNA substrates in a reversible reaction that generates 2',3'-cyclic phosphate and 5'-hydroxyl termini. The hairpin catalytic motif was discovered in the negative strand of the tobacco ringspot virus satellite RNA, where hairpin ribozyme-mediated self-cleavage and ligation ...
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Hairpin ribozyme mediated RNA recombination
Chemical Communications, 2016An engineered hairpin ribozyme supports the recombination of two non-functional substrates into a functional hammerhead ribozyme.
Robert Hieronymus +3 more
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Kinetic Characterization of Hairpin Ribozyme Variants [PDF]
, 2012 Kinetic analysis of ribozyme reactions is a common method to evaluate and compare activities of catalytic RNAs. The hairpin ribozyme catalyzes the reversible cleavage of a suitable RNA substrate at a specific site. Hairpin ribozyme variants as an allosteric ribozyme responsive to flavine mononucleotide and a hairpin-derived twin ribozyme that catalyzes
Thomas Marschall +3 more
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Crystallization of the Hairpin Ribozyme: Illustrative Protocols
2004Conditions and techniques that result in successful crystallization differ from RNA to RNA. However, there are some general principles that facilitate crystallization of most RNAs. Three procedures that were instrumental in obtaining well-ordered crystals of the hairpin ribozyme are described in this chapter.
Adrian R. Ferré-D'Amaré +1 more
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The catalytic mechanism of the hairpin ribozyme
Biochemical Society Transactions, 2002Evidence that hairpin ribozymes function in the absence of bivalent cation cofactors suggests that active site nucleobases might participate directly in catalytic chemistry. We have adopted an abasic ribozyme rescue strategy to begin to dissect the roles of specific nucleobases in hairpin ribozyme activity. Loss of one active site nucleobase, G8, could
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ChemInform Abstract: Origins of RNA Catalysis in the Hairpin Ribozyme [PDF]
ChemInform, 2001 AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
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Design and Function of Triplex Hairpin Ribozymes
2010Triplex ribozymes allow for the individual activity of multiple trans-acting ribozymes producing higher target cleavage relative to tandem-expressed RZs. A triplex expression system based on a single hairpin ribozyme for the multiple expression (multiplex) vectors can be engineered to target RNAs with single or multiple antisense-accessible sites ...
Ramiro Rojas-Hernández +2 more
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