Results 191 to 200 of about 59,552 (223)
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1994
RNA enzymes, or ribozymes, are fascinating molecules with novel biochemical properties. Since the discovery of ribozymes in 1981, biological catalysis by RNA has been found to be at the heart of critical steps in gene expression, including RNA splicing, endonucleolytic RNA processing, and possibly ribosomal peptide bond formation (Sheldon et al.
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RNA enzymes, or ribozymes, are fascinating molecules with novel biochemical properties. Since the discovery of ribozymes in 1981, biological catalysis by RNA has been found to be at the heart of critical steps in gene expression, including RNA splicing, endonucleolytic RNA processing, and possibly ribosomal peptide bond formation (Sheldon et al.
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Self-association of adenine-dependent hairpin ribozymes
European Biophysics Journal, 2007Hairpin ribozymes are flexible molecules that catalyse reversible self-cleavage after the docking of two independently folded internal loops, A and B. The activities, self-association and structures in solution of two 85 base adenine-dependent hairpin ribozymes (ADHR1 and ADHR2) were studied by native gel electrophoresis, analytical centrifugation, and
Li, Yan-Li +5 more
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Kinetics and Thermodynamics of Intermolecular Catalysis by Hairpin Ribozymes
Biochemistry, 1995The hairpin ribozyme, derived from the negative strand of the satellite RNA of tobacco ringspot virus, belongs to the class of small catalytic RNAs that cleave RNA to generate 2',3'-cyclic phosphate and 5'hydroxyl termini and ligate these termini in the reverse reaction to form 3',5'-phosphodiesters.
Martha J. Fedor, Lisa A. Hegg
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Impedimetric Detection of Hairpin Ribozyme Activity
, 2011Electrochemical impedance spectroscopy is successfully utilized for label-free monitoring of the cleavage reaction of a RNA substrate by a complementary hairpin ribozyme.
Katarzyna M. Piekielska +4 more
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Simulation-Guided Conformational Space Exploration to Assess Reactive Conformations of a Ribozyme.
Journal of Chemical Theory and ComputationSelf-splicing ribozymes are small ribonucleic acid (RNA) enzymes that catalyze their own cleavage through a transphosphoesterification reaction. While this process is involved in some specific steps of viral RNA replication and splicing, it is also of ...
Sélène Forget +3 more
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Enhanced Folding of Hairpin Ribozymes with Replaced Domains
Biochemistry, 1996Reversely joined ribozymes (Komatsu et al., 1995) have been proven to be active. Here we describe the construction of hairpin ribozymes with separated domains, but containing complementary arms for association of the two domains. Linker nucleotides were inserted between the arms and domains. These ribozymes were active under the standard conditions (12
Eiko Ohtsuka +2 more
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Towards Higher Complexity in the RNA World: Hairpin Ribozyme Supported RNA Recombination
, 2021R. Hieronymus, S. Müller
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A Mechanistic Comparison of the Varkud Satellite and Hairpin Ribozymes
2013The hairpin and Varkud satellite ribozymes are two members of the class of nucleolytic ribozymes that catalyze cleavage and ligation reactions at a specific site. Cleavage occurs by a transesterification reaction whereby the 2'-O attacks the adjacent phosphorus with departure of the 5'-O to leave a 2',3'-cyclic phosphate.
Wilson, Timothy J., Lilley, David M. J.
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The hairpin ribozyme: from crystal structure to function
Biochemical Society Transactions, 2002The hairpin ribozyme is one of four known natural catalytic RNAs that carry out sequence-specific cleavage of RNA. It is of particular biochemical interest because, unlike ‘classic’ ribozymes, such as the group I intron, it appears not to employ metal ions as catalytic cofactors.
P. B. Rupert, A. R. Ferré-D'Amaré
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Hairpin and hammerhead ribozymes: how different are they?
Biochemical Society Transactions, 2002Recent experimental work on the hairpin and hammerhead ribozymes suggests that they have more similarities than previously suspected. Notably, each is now known to function as a true RNA catalyst, not requiring metal ions for folding or catalytic function.
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