Results 201 to 210 of about 59,552 (223)

Structural Basis for the Guanosine Requirement of the Hairpin Ribozyme

Biochemistry, 1999
To form a catalytically active complex, the essential nucleotides of the hairpin ribozyme, embedded within the internal loops of the two domains, must interact with one another. Little is known about the nature of these essential interdomain interactions.
Dominic Lambert, Joyce E. Heckman, Robert Pinard, Nils G. Walter, François Major, John M. Burke   +5 more
openaire   +3 more sources

Two RNA Folds from One Sequence: A Ribozyme with Versatile Substrate Processing Abilities.

Angewandte Chemie
We report the design of a single RNA sequence capable of adopting one of two ribozyme folds and catalyzing the cleavage and/or ligation of the respective substrates.
Sabine Müller, Jikang Zhu, Dorothea Dierks, Christina Möller, Darko Balke   +4 more
semanticscholar   +1 more source

Design and Optimization of Sequence-Specific Hairpin Ribozymes

2004
The hairpin ribozyme belongs to a group of small catalytic RNAs that have been extensively used to trans-cleave RNA molecules. Many efforts have been made to elucidate its reaction mechanism, and there is great interest in designing hairpin ribozymes with improved catalytic activity for use in the development of agents that specifically inactivate RNA ...
Alicia Barroso-delJesus, Alfredo Berzal-Herranz, Cristina Romero-López, Elena Puerta-Fernñndez   +3 more
openaire   +3 more sources

The Hairpin and Varkud Satellite Ribozymes

2007
Nucleolytic ribozymes carry out cleavage at a specific site by transesterification reactions in which the 2′-oxygen attacks the 3′-phosphorus, with departure of the 5′-oxygen to leave a cyclic 2′-3′-phosphate. The reaction follows an SN2 mechanism, with inversion of configuration at the phosphorus.
openaire   +2 more sources

Kinetics of hairpin ribozyme cleavage in yeast.

RNA (New York, N.Y.), 1997
Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast.
Donahue, Christine Patricia, Fedor, Martha J.   +1 more
openaire   +2 more sources

The Hairpin Ribozyme: Discovery and Development for Gene Therapy

2003
The hairpin ribozyme is a member of a family of small RNA endonucleases, which includes hammerhead, human hepatitis delta virus, Neurospora VS, and the lead-dependent catalytic RNAs. All these catalytic RNAs reversibly cleave the phosphodiester bond of substrate RNA to generate 5′-hydroxyl and 2′,3′-cyclic phosphate termini.
openaire   +2 more sources

Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes

2004
The application of conventional enzymological methods to the study of hairpin and hammerhead ribozymes has led to valuable insights into the mechanisms by which these small RNAs catalyze phosphodiester cleavage and ligation reactions. Here, protocols are presented for measuring rate constants for simple cleavage and ligation reactions mediated by ...
openaire   +3 more sources

In Vitro Selection of Hairpin Ribozymes

2003
Bruno Sargueil, John M. Burke
openaire   +3 more sources

Mechanistic studies of the hairpin ribozyme

Collection of Czechoslovak Chemical Communications, 1996
Francesca Gill, Jane A. Grasby, Karen J. Young   +2 more
openaire   +2 more sources

Ligation of RNA Molecules by the Hairpin Ribozyme

2003
Alfredo Bertal-Herranz, John M. Burke
openaire   +3 more sources