Catalytic importance of a protonated adenosine in the hairpin ribozyme active site. [PDF]
Biochemistry, 2010 The hairpin ribozyme accelerates the rate of phosphodiester transfer reactions by at least 5 orders of magnitude. To achieve this rate enhancement, the active site forms via a substrate helix docking event that constrains the scissile phosphate linkage ...
Suydam IT, Levandoski SD, Strobel SA.
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QM/MM studies of hairpin ribozyme self-cleavage suggest the feasibility of multiple competing reaction mechanisms. [PDF]
J Phys Chem B, 2011 The hairpin ribozyme is a prominent member of small ribozymes since it does not require metal ions to achieve catalysis. Guanine 8 (G8) and adenine 38 (A38) have been identified as key participants in self-cleavage and -ligation.
Mlýnský V +4 more
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A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture [PDF]
RNA BiologySelf-cleaving ribozymes are versatile tools for synthetic biologists when it comes to controlling gene expression. Up to date, 12 different classes are known, and over the past decades more and more details about their structure, cleavage mechanisms and ...
Dennis Kläge +2 more
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Cobalt(III)hexaammine-dependent photocrosslinks in the hairpin ribozyme. [PDF]
J Inorg Biochem, 2014 Kraemer-Chant CM +3 more
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A triplex ribozyme expression system based on a single hairpin ribozyme. [PDF]
Oligonucleotides, 2008 Triplex ribozyme (RZ) configurations allow for the individual activity of trans-acting RZs in multiple expression cassettes (multiplex), thereby increasing target cleavage relative to conventionally expressed RZs. Although hairpin RZs have been advantageously compared to hammerhead RZs, their longer size and structural features complicated triplex ...
Aquino-Jarquin G +3 more
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The many faces of the hairpin ribozyme: Structural and functional variants of a small catalytic rna [PDF]
IUBMB Life - A Journal of the International Union of Biochemistry and Molecular Biology, 2011 Sabine Müller +3 more
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Thermodynamics and kinetics of the hairpin ribozyme from atomistic folding/unfolding simulations. [PDF]
J Mol Biol, 2011 Nivón LG, Shakhnovich EI.
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Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells
Viruses, 2021 Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey +3 more
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Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain. [PDF]
PLoS ONE, 2018 The natural trans-acting ribozyme RNase P RNA (RPR) is composed of two domains in which the catalytic (C-) domain mediates cleavage of various substrates. The C-domain alone, after removal of the second specificity (S-) domain, catalyzes this reaction as
Guanzhong Mao +5 more
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