Cobalt(III)hexaammine-dependent photocrosslinks in the hairpin ribozyme. [PDF]
J Inorg Biochem, 2014 Kraemer-Chant CM +3 more
europepmc +2 more sources
A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture [PDF]
RNA BiologySelf-cleaving ribozymes are versatile tools for synthetic biologists when it comes to controlling gene expression. Up to date, 12 different classes are known, and over the past decades more and more details about their structure, cleavage mechanisms and ...
Dennis Kläge +2 more
doaj +2 more sources
The many faces of the hairpin ribozyme: Structural and functional variants of a small catalytic rna [PDF]
IUBMB Life - A Journal of the International Union of Biochemistry and Molecular Biology, 2011 Sabine Müller +3 more
openalex +2 more sources
Functional Domain-Assembly in Hairpin Ribozymes [PDF]
Journal of Biochemistry, 2000 Functional structures of hairpin ribozymes have been investigated by constructing various chemically modified molecules. Domain-exchange and linker insertion experiments were performed to find active conformations of the RNA enzyme showing cleavage activity.
Yasuo Komatsu +5 more
openalex +4 more sources
Intracellular Immunization against SIVmacUtilizing a Hairpin Ribozyme
Virology, 1996 A hairpin ribozyme targeting the 3' LTR region (9456) of SIVmac238 was cloned into a murine retroviral vector. This target sequence is conserved among various SIV, as well as most HIV-2, strains. The ribozyme cassette is driven from a polymerase III promoter, that of the human tRNAval gene.
Marina Heusch +3 more
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Thermodynamics and kinetics of the hairpin ribozyme from atomistic folding/unfolding simulations. [PDF]
J Mol Biol, 2011 Nivón LG, Shakhnovich EI.
europepmc +2 more sources
Nucleobase catalysis in the hairpin ribozyme [PDF]
RNA, 2006 RNA catalysis is important in the processing and translation of RNA molecules, yet the mechanisms of catalysis are still unclear in most cases. We have studied the role of nucleobase catalysis in the hairpin ribozyme, where the scissile phosphate is juxtaposed between guanine and adenine bases.
Timothy J. Wilson +6 more
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Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells
Viruses, 2021 Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey +3 more
doaj +1 more source
Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain. [PDF]
PLoS ONE, 2018 The natural trans-acting ribozyme RNase P RNA (RPR) is composed of two domains in which the catalytic (C-) domain mediates cleavage of various substrates. The C-domain alone, after removal of the second specificity (S-) domain, catalyzes this reaction as
Guanzhong Mao +5 more
doaj +1 more source