Results 21 to 30 of about 8,406 (222)
Critical domain interactions for type A RNase P RNA catalysis with and without the specificity domain. [PDF]
PLoS ONE, 2018 The natural trans-acting ribozyme RNase P RNA (RPR) is composed of two domains in which the catalytic (C-) domain mediates cleavage of various substrates. The C-domain alone, after removal of the second specificity (S-) domain, catalyzes this reaction as
Guanzhong Mao +5 more
doaj +1 more source
RNA interference approaches for treatment of HIV-1 infection. [PDF]
, 2015 HIV/AIDS is a chronic and debilitating disease that cannot be cured with current antiretroviral drugs. While combinatorial antiretroviral therapy (cART) can potently suppress HIV-1 replication and delay the onset of AIDS, viral mutagenesis often leads to
Bobbin, Maggie L +2 more
core +1 more source
A modern mode of activation for nucleic acid enzymes.
PLoS ONE, 2007 Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficiently high substrate specificity required by modern cellular metabolism. One of these modes is the use of a target-dependent module (i.e. a docking domain)
Dominique Lévesque +2 more
doaj +1 more source
Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases [PDF]
, 2004 For two centuries, vaccination has been the dominating approach to develop prophylaxis against viral infections through immunological prevention. However, vaccines are not always possible to make, are ineffective for many viral infections, and also carry
Fang, Fang, Le Calvez, Hervé, Yu, Mang
core +3 more sources
Influence of a 3′ Terminal Ribozyme on AgoshRNA Biogenesis and Activity
Molecular Therapy: Nucleic Acids, 2019 Short hairpin RNAs (shRNAs) can induce gene silencing via the RNA interference (RNAi) mechanism. We designed an alternative shRNA molecule with a relatively short base-paired stem that bypasses Dicer and instead is processed by the Argonaute 2 (Ago2 ...
Elena Herrera-Carrillo +2 more
doaj +1 more source
RNA Back and Forth: Looking through Ribozyme and Viroid Motifs
Viruses, 2019 Current cellular facts allow us to follow the link from chemical to biochemical metabolites, from the ancient to the modern world. In this context, the “RNA world„ hypothesis proposes that early in the evolution of life, the ribozyme was ...
Marie-Christine Maurel +3 more
doaj +1 more source
Force for ancient and recent life: viral and stem-loop RNA consortia promote life. [PDF]
, 2014 Lytic viruses were thought to kill the most numerous host (i.e., kill the winner). But persisting viruses/defectives can also protect against viruses, especially in a ubiquitous virosphere. In 1991, Yarmolinsky et al.
Villarreal, Luis P
core +1 more source
Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis
Angewandte Chemie, EarlyView.Ribozymes enable sequence‐encoded turnover of RNA droplet material, resulting in transiently active droplets that selectively segregate based on their genotype. This establishes a direct link between genetic information and droplet behavior, marking a step toward programmable, evolvable RNA‐based compartments with life‐like properties.
Franziska Giessler +5 more
wiley +2 more sources
BMC Biotechnology, 2011 Background The group I intron, a ribozyme that catalyzes its own splicing reactions in the absence of proteins in vitro, is a potential target for rational engineering and attracted our interest due to its potential utility in gene repair using trans ...
Takaku Hiroshi +2 more
doaj +1 more source
How RNA acts as a nuclease:some mechanistic comparisons in the nucleolytic ribozymes [PDF]
, 2017 Recent structural and mechanistic studies have shed considerable light on the catalytic mechanisms of nucleolytic ribozymes. The discovery of several new ribozymes in this class has now allowed comparisons to be made, and the beginnings of mechanistic ...
Lilley, David M. J.
core +2 more sources