Results 31 to 40 of about 59,552 (223)
Screening for Important Base Identities in the Hairpin Ribozyme by In Vitro Selection for Cleavage
BioTechniques, 1998 Random mutagenesis followed by an in vitro selection procedure was shown to be capable of identifying important bases of the hairpin ribozyme for cleavage of an RNA target sequence.
Andrew Siwkowski +3 more
doaj +1 more source
RNA interference approaches for treatment of HIV-1 infection. [PDF]
, 2015 HIV/AIDS is a chronic and debilitating disease that cannot be cured with current antiretroviral drugs. While combinatorial antiretroviral therapy (cART) can potently suppress HIV-1 replication and delay the onset of AIDS, viral mutagenesis often leads to
Bobbin, Maggie L +2 more
core +1 more source
A modern mode of activation for nucleic acid enzymes.
PLoS ONE, 2007 Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficiently high substrate specificity required by modern cellular metabolism. One of these modes is the use of a target-dependent module (i.e. a docking domain)
Dominique Lévesque +2 more
doaj +1 more source
Folding and catalysis by the hairpin ribozyme [PDF]
FEBS Letters, 1999 The hairpin ribozyme undergoes a site‐specific transesterification cleavage of the phosphodiester backbone. The natural form of the ribozyme is a four‐way helical junction, where two arms contain unpaired loops. This folds by pairwise coaxial stacking of helical arms, and a rotation into an antiparallel conformation in which there is close association ...
openaire +3 more sources
Molecular Therapy: Nucleic Acids, 2014 Antisense-based molecules targeting HIV-1 RNA have the potential to be used as part of gene or drug therapy to treat HIV-1 infection. In this study, HIV-1 RNA was screened to identify more conserved and accessible target sites for ribozymes based on the ...
Robert J Scarborough +7 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA ...
Tomasz Czapik +3 more
doaj
BMC Biotechnology, 2011 Background The group I intron, a ribozyme that catalyzes its own splicing reactions in the absence of proteins in vitro, is a potential target for rational engineering and attracted our interest due to its potential utility in gene repair using trans ...
Takaku Hiroshi +2 more
doaj +1 more source
The folding of the hairpin ribozyme: Dependence on the loops and the junction [PDF]
RNA, 2000 In its natural context, the hairpin ribozyme is constructed around a four-way helical junction. This presents the two loops that interact to form the active site on adjacent arms, requiring rotation into an antiparallel structure to bring them into proximity.
Zheng-yun Zhao +3 more
openalex +5 more sources
Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases [PDF]
, 2004 For two centuries, vaccination has been the dominating approach to develop prophylaxis against viral infections through immunological prevention. However, vaccines are not always possible to make, are ineffective for many viral infections, and also carry
Fang, Fang, Le Calvez, Hervé, Yu, Mang
core +3 more sources
RNA Back and Forth: Looking through Ribozyme and Viroid Motifs
Viruses, 2019 Current cellular facts allow us to follow the link from chemical to biochemical metabolites, from the ancient to the modern world. In this context, the “RNA world„ hypothesis proposes that early in the evolution of life, the ribozyme was ...
Marie-Christine Maurel +3 more
doaj +1 more source