Results 31 to 40 of about 8,406 (222)

Mutagenesis of the hairpin ribozyme

Nucleic Acids Research, 1994
Extensive in vitro mutagenesis studies have been performed on the hairpin ribozyme and substrate in an effort to refine the overall secondary structure of the molecule and provide further insight into what elements are essential for activity. A secondary structure consisting of four helices and five loop regions remains the basic model as originally ...
P, Anderson, J, Monforte, R, Tritz, S, Nesbitt, J, Hearst, A, Hampel   +5 more
openaire   +3 more sources

A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination [PDF]

, 2010
RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic Fab library displayed on phage to obtain Fabs
A Desmyter, A Ke, A Serganov, AA Kossiakoff, AC Messias, AL Edwards, Anthony A Kossiakoff, AR Ferré-D'Amaré, AR Ferré-D'Amaré, AY Keel, BL Golden, BM Lunde, BS Saville, C Eigenbrot, C Höbartner, C Keryer-Bibens, CG Hoogstraten, D Piekna-Przybylska, David M Shechner, David P Bartel, DM Shechner, EA Kabat, EH Ekland, Erica M Duguid, FA Fellouse, FA Fellouse, FA Fellouse, IA Laird-Offringa, J-D Ye, JA Chao, JA Doudna, Jingdong Ye, Joseph A Piccirilli, JR Hogg, JR Hogg, JS Mattick, JS Mattick, K Decanniere, M Jinek, MJ Fedor, MP Robertson, N Carrasco, NH Bergman, Nikolai B Suslov, P Kapranov, PJ Carter, R Dutzler, RJ Taft, RK Montange, S Birtalan, S Iwata, S Jones, S Koide, S Koide, S Uysal, Sachdev S Sidhu, SC Bagby, Shohei Koide, SL Reichow, SR Eddy, SS Sidhu, TA Cooper, V Tereshko, Yelena Koldobskaya   +63 more
core   +2 more sources

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]

, 2020
Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL, Block, Steven M, Greenleaf, William J, Savinov, Andrew   +3 more
core  

CCR5 Targeted Cell Therapy for HIV and Prevention of Viral Escape

Viruses, 2015
Allogeneic transplantation with CCR5-delta 32 (CCR5-d32) homozygous stem cells in an HIV infected individual in 2008, led to a sustained virus control and probably eradication of HIV.
Gero Hütter, Josef Bodor, Scott Ledger, Maureen Boyd, Michelle Millington, Marlene Tsie, Geoff Symonds   +6 more
doaj   +1 more source

Intracellular RNA cleavage by the hairpin ribozyme [PDF]

Nucleic Acids Research, 1998
Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complicated by recent results indicating ...
A A, Seyhan, J, Amaral, J M, Burke
openaire   +2 more sources

Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III

Molecular Therapy: Nucleic Acids, 2018
Type 3 Pol III promoters such as U6 are widely used for expression of small RNAs, including short hairpin RNA for RNAi applications and guide RNA in CRISPR genome-editing platforms. RNA polymerase III uses a T-stretch as termination signal, but the exact
Zongliang Gao, Elena Herrera-Carrillo, Ben Berkhout   +2 more
doaj   +1 more source

Equilibrium properties and force-driven unfolding pathways of RNA molecules

, 2009
The mechanical unfolding of a simple RNA hairpin and of a 236--bases portion of the Tetrahymena thermophila ribozyme is studied by means of an Ising--like model.
A. Imparato, A. Pelizzola, M. Müller, M. Zamparo   +3 more
core   +1 more source

Crystallographic Study of DNA T‐Junction via Crystal Engineering

Angewandte Chemie, Volume 137, Issue 51, December 15, 2025.
Engineered DNA crystals provides a platform to regularly arrange guest molecules for crystallographic study. Herein, the 3D structure of T‐junction, a commonly used motif in DNA nanotechnology, was revealed. Abstract Engineering DNA crystals is the primary motivation for structural DNA nanotechnology.
Xiang Li, Naseem Siraj, Ruojie Sha, Chengde Mao   +3 more
wiley   +2 more sources

Self-assembly Controls Self-cleavage of HHR from ASBVd (−): a Combined SANS and Modeling Study [PDF]

, 2016
International audienceIn the Avocado Sunblotch Viroid (ASBVd: 249-nt) from the Avsunviroidae family, a symmetric rolling-circle replication operates through an autocatalytic mechanism mediated by hammerhead ribozymes (HHR) embedded in both polarity ...
Leclerc, Fabrice, Martel, Anne, Maurel, Marie-Christine, Vergne, Jacques, Zaccai, Giuseppe, Řìhovà, Martina   +5 more
core   +3 more sources

Capturing the essence of folding and functions of biomolecules using Coarse-Grained Models

, 2011
The distances over which biological molecules and their complexes can function range from a few nanometres, in the case of folded structures, to millimetres, for example during chromosome organization. Describing phenomena that cover such diverse length,
A Dhar, A Elcock, A Elcock, A Grosberg, A Grosberg, A Minton, A Pisliakov, A Podtelezhnikov, A Rychkova, A Savelyev, A Vologodskii, AA Podtelezhnikov, B Messer, BJ Alder, C Bustamante, C Clementi, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, D Thirumalai, D Thirumalai, D Thirumalai, D Thirumalai, D Thirumalai, E Koculi, E Lieberman-Aiden, E O'Brien, E Shakhnovich, EP O'Brien, F Valle, G Lorimer, G Papoian, G Ziv, H Liu, H Ma, HX Zhou, I Tinoco Jr., J Chen, J Harms, J Karanicolas, J Straub, JD Honeycutt, JM Fernandez, JN Onuchic, KA Dill, M Levitt, M Mickler, M Strajbl, M Takano, ME Fisher, MJ Todd, MS Cheung, N Hirokawa, N Koga, N Toan, OK Dudko, P Weinkam, PB Moore, PC Whitford, Q Du, R Russell, R Tehver, R Tehver, RD Vale, RD Vale, RI Dima, RW Pastor, S Jun, S Jun, S Jun, S Kamerlin, S Kirkpatrick, S Moghaddam, SB Smith, SJ Chen, SJ Marrink, T Cloutier, T Cremer, T Kirkpatrick, T Veitshans, V Lubchenko, Z Liu, Z Xu   +89 more
core   +1 more source