Role of tautomerism in RNA biochemistry [PDF]
, 2015 Heterocyclic nucleic acid bases and their analogs can adopt multiple tautomeric forms due to the presence of multiple solvent-exchangeable protons.
Essigmann, John M. +2 more
core +1 more source
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]
, 2020 Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL +3 more
core
A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination [PDF]
, 2010 RNA crystallization and phasing represent major bottlenecks in RNA structure determination. Seeking to exploit antibody fragments as RNA crystallization chaperones, we have used an arginine-enriched synthetic Fab library displayed on phage to obtain Fabs
A Desmyter +63 more
core +2 more sources
CCR5 Targeted Cell Therapy for HIV and Prevention of Viral Escape
Viruses, 2015 Allogeneic transplantation with CCR5-delta 32 (CCR5-d32) homozygous stem cells in an HIV infected individual in 2008, led to a sustained virus control and probably eradication of HIV.
Gero Hütter +6 more
doaj +1 more source
Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III
Molecular Therapy: Nucleic Acids, 2018 Type 3 Pol III promoters such as U6 are widely used for expression of small RNAs, including short hairpin RNA for RNAi applications and guide RNA in CRISPR genome-editing platforms. RNA polymerase III uses a T-stretch as termination signal, but the exact
Zongliang Gao +2 more
doaj +1 more source
Intracellular RNA cleavage by the hairpin ribozyme [PDF]
Nucleic Acids Research, 1998 Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complicated by recent results indicating ...
Attila A. Seyhan +2 more
openaire +3 more sources
Lipid vesicles chaperone an encapsulated RNA aptamer. [PDF]
, 2018 The organization of molecules into cells is believed to have been critical for the emergence of living systems. Early protocells likely consisted of RNA functioning inside vesicles made of simple lipids.
Chen, Irene A +2 more
core +1 more source
Equilibrium properties and force-driven unfolding pathways of RNA molecules
, 2009 The mechanical unfolding of a simple RNA hairpin and of a 236--bases portion of the Tetrahymena thermophila ribozyme is studied by means of an Ising--like model.
A. Imparato +3 more
core +1 more source
Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit
Angewandte Chemie, Volume 137, Issue 29, July 14, 2025.A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo +7 more
wiley +2 more sources
RNA under Tension: Folding Landscapes, Kinetic Partitioning Mechanism, and Molecular Tensegrity
, 2012 Non-coding RNA sequences play a great role in controlling a number of cellular functions, thus raising the need to understand their complex conformational dynamics in quantitative detail.
Hyeon, Changbong +2 more
core +1 more source