Ribozyme-based insulator parts buffer synthetic circuits from genetic context [PDF]
, 2012 Synthetic genetic programs are built from circuits that integrate sensors and implement temporal control of gene expression. Transcriptional circuits are layered by using promoters to carry the signal between circuits. In other words, the output promoter
A Arkin +48 more
core +1 more source
RNA under Tension: Folding Landscapes, Kinetic Partitioning Mechanism, and Molecular Tensegrity
, 2012 Non-coding RNA sequences play a great role in controlling a number of cellular functions, thus raising the need to understand their complex conformational dynamics in quantitative detail.
Hyeon, Changbong +2 more
core +1 more source
Searching for a DNAzyme version of the leadzyme [PDF]
, 2015 The final publication is available at Springer via http://dx.doi.org/10.1007/s00239-015-9702-zThe leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. In an optimized form, the enzyme strand contains only two unpaired
Chen, Qingyun +2 more
core +1 more source
Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi] [PDF]
Türk Biyokimya Dergisi, 2006 Self-processing hairpin ribozymes have been synthesized from promoterless singlestranded DNA circles (73 nt) within mammalian cells. Following lipid-mediated transient transfection, DNA circles were efficiently internalized by mouse L cells (OST7–1 ...
Attila A. Seyhan
doaj
Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications
Chemistry – A European Journal, EarlyView.Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley +1 more source
Folding and catalysis by the hairpin ribozyme [PDF]
FEBS Letters, 1999 The hairpin ribozyme undergoes a site‐specific transesterification cleavage of the phosphodiester backbone. The natural form of the ribozyme is a four‐way helical junction, where two arms contain unpaired loops. This folds by pairwise coaxial stacking of helical arms, and a rotation into an antiparallel conformation in which there is close association ...
openaire +2 more sources
The prebiotic evolutionary advantage of transferring genetic information from RNA to DNA. [PDF]
, 2011 In the early 'RNA world' stage of life, RNA stored genetic information and catalyzed chemical reactions. However, the RNA world eventually gave rise to the DNA-RNA-protein world, and this transition included the 'genetic takeover' of information storage ...
Chen, Irene A +4 more
core +4 more sources
Aggregate, Volume 7, Issue 2, February 2026.This study presents a dynamic‐responsive sensing system composed of RNA aptamers and polymers for adaptive histamine sensing. Programmable RNA self‐assembly via PEG8K enables phase‐separated condensates with enhanced stability and affinity. A label‐free FRET system (ThT→HBC dyes) achieves high‐sensitive detection (15.36 nM) in complex matrices ...
Yifan Wu +7 more
wiley +1 more source
Meeting review : ESF workshop on "Impact of nucleic acid chemistry on gene function analysis: antisense, aptamers, ribozymes and RNAi" [PDF]
, 2008 The shortage of functional information compared to the abundance of sequence information characterizes today’s situation in functional genomics. For many years the knock-down of a gene’s product has been the most powerful way of analysing its function ...
Engels, Joachim W. (Prof. Dr.) +3 more
core
Crystal structure of a tRNA acceptor‐stem mimic at 1.94 Å resolution
Acta Crystallographica Section F, Volume 82, Issue 2, Page 57-65, February 2026.This paper reports the structure solution of an RNA complex using anomalous scattering.Despite the theoretical advantages of phosphorus single‐wavelength anomalous diffraction (P‐SAD) for nucleic acid phasing, its application remains limited due to high atomic displacement parameters and an unfavourable ratio of unique reflections to anomalous ...
Ziwei Liu +5 more
wiley +1 more source