Results 71 to 80 of about 8,461 (225)
Engineering an optimized hypercompact CRISPR/Cas12j‐8 system for efficient genome editing in plants
Plant Biotechnology Journal, Volume 23, Issue 4, Page 1153-1164, April 2025.Summary The Cas12j‐8 nuclease, derived from the type V CRISPR system, is approximately half the size of Cas9 and recognizes a 5′‐TTN‐3′ protospacer adjacent motif sequence, thus potentially having broad application in genome editing for crop improvement. However, its editing efficiency remains low in plants. In this study, we rationally engineered both
Shasha Bai +5 more
wiley +1 more source
Ion Condensation onto Ribozyme is Site-Specific and Fold-Dependent [PDF]
, 2019 The highly charged RNA molecules, with each phosphate carrying a single negative charge, cannot fold into well-defined architectures with tertiary interactions, in the absence of ions. For ribozymes, divalent cations are known to be more efficient than monovalent ions in driving them to a compact state although Mg$^{2+}$ ions are needed for catalytic ...
arxiv +1 more source
Influence of a 3′ Terminal Ribozyme on AgoshRNA Biogenesis and Activity
Molecular Therapy: Nucleic Acids, 2019 Short hairpin RNAs (shRNAs) can induce gene silencing via the RNA interference (RNAi) mechanism. We designed an alternative shRNA molecule with a relatively short base-paired stem that bypasses Dicer and instead is processed by the Argonaute 2 (Ago2 ...
Elena Herrera-Carrillo +2 more
doaj
Factors determining chromosomal localization of transposable elements in plants
Plant Biology, EarlyView.We review transposon chromosomal niches, and the functional consequences of their specific chromosomal localization in plants. Abstract Transposable elements (TEs) constitute a significant part of plant genomes and shape their genomic landscape. While some TEs are ubiquitously dispersed, other elements specifically occupy discrete genomic loci.
E. Kejnovsky +3 more
wiley +1 more source
ChemBioChem, Volume 26, Issue 10, May 27, 2025.Rational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Constanze Ebermann, Sabine Müller
wiley +1 more source
Specificity of the Hairpin Ribozyme [PDF]
Journal of Biological Chemistry, 1999 Substrate sequence requirements of the hairpin ribozyme have been partially defined by both mutational and in vitro selection experiments. It was considered that the best targets were those that included the N downward arrowGUC sequence surrounding the cleavage site.
Mercedes Pérez-Ruiz +2 more
openaire +3 more sources
Meeting review : ESF workshop on "Impact of nucleic acid chemistry on gene function analysis: antisense, aptamers, ribozymes and RNAi" [PDF]
, 2008 The shortage of functional information compared to the abundance of sequence information characterizes today’s situation in functional genomics. For many years the knock-down of a gene’s product has been the most powerful way of analysing its function ...
Engels, Joachim W. (Prof. Dr.) +3 more
core
Hairpin vortices and heat transfer in the wakes behind two hills with different scales [PDF]
arXiv, 2023 This study performed a numerical analysis of the hairpin vortex and heat transport generated by the interference of the wakes behind two hills in a laminar boundary layer. In the case of hills with the same scale, the interference between hairpin vortices in the wake is more intensive than in the different-scale hills.
arxiv
Small, Volume 21, Issue 9, March 5, 2025.Microfluidic‐based directed evolution is a powerful tool for selection of biopolymers, however they struggle in transferability to intracellular environments. The “biomimetic equivalence” principle provides a versatile and practical framework for developing biopolymers that function effectively in cells using a cell‐free approach.
Andrew Brian Kinghorn +10 more
wiley +1 more source
A posteriori design of crystal contacts to improve the X-ray diffraction properties of a small RNA enzyme [PDF]
, 2007 Insertion of a dangling 5′-uracil and incorporation of synthetic linkers at the domain interface of a minimal hairpin ribozyme have been investigated as means of favorably influencing crystal packing. These modifications lead to changes in the ribozyme’s
Krucinska, Jolanta +3 more
core +3 more sources