Results 1 to 10 of about 1,180,341 (309)

Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity

Scientific Reports, 2023
The utilization of nanotechnology and biotechnology for enhancing the synthesis of plant bioactive chemicals is becoming increasingly common. The hairy root culture technique can be used to increase secondary metabolites such as tropane alkaloids ...
Aisha M. Abdelkawy, Shifaa O. Alshammari, Hebat-Allah A. Hussein, Inas M. M. Abou El-Enain, Eman S. Abdelkhalek, Asmaa M. Radwan, Sahar K. M. Kenawy, Doaa A. M. Maaty, Nermine N. Abed, Shadia Sabry, Abeer Mohsen   +10 more
doaj   +2 more sources

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean

Plant Methods, 2021
Background Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology.
Yuanyuan Cheng, Xiaoli Wang, Li Cao, Jing Ji, Tengfei Liu, Kaixuan Duan   +5 more
doaj   +2 more sources

Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin [PDF]

Brazilian Journal of Pharmaceutical Sciences, 2020
Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop
Baranski, Rafal, Combik, Michal, Khlifa, Heba Desouky, Klimek-Chodacka, Magdalena, Taha, Hussein Sayed   +4 more
core   +4 more sources

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Frontiers in Plant Science, 2023
Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants.
Diptesh Biswas, Avijit Chakraborty, Swapna Mukherjee, Biswajit Ghosh   +3 more
doaj   +2 more sources

Agrobacterium rhizogenes-mediated hairy root transformation as an efficient system for gene function analysis in Litchi chinensis

Plant Methods, 2021
Background Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable transformation.
Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu, Jietang Zhao   +6 more
doaj   +2 more sources

Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012
Transgenic hairy root system is a promising source of secondary metabolites in medicinal plants with high pharmaceutical value.For the first time, hairy roots were established in different explants of Linum mucronatum, an anti-cancer agent producing ...
Afsaneh SAMADI, Jirair CARAPETIAN, Reza HEIDARI, Morad JAFARI, Abdollah HASSANZADEH GORTTAPEH   +4 more
doaj   +3 more sources

An Efficient Hairy Root System for Validation of Plant Transformation Vector and CRISPR/Cas Construct Activities in Cucumber (Cucumis sativus L.)

Frontiers in Plant Science, 2022
Hairy root induction system has been applied in various plant species as an effective method to study gene expression and function due to its fast-growing and high genetic stability.
Doai Van Nguyen, Doai Van Nguyen, Trang Thi-Huyen Hoang, Ngoc Thu Le, Huyen Thi Tran, Cuong Xuan Nguyen, Yong-Hwan Moon, Yong-Hwan Moon, Ha Hoang Chu, Ha Hoang Chu, Phat Tien Do, Phat Tien Do   +11 more
doaj   +2 more sources

Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production [PDF]

, 2011
Background The therapeutic and health promoting role of highly unsaturated fatty acids (HUFAs) from fish, i.e. eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) are well known.
Cequier-Sánchez, Elena, Dorta-Guerra, Roberto, Ravelo, Ángel G, Rodríguez, Covadonga, Zárate, Rafael   +4 more
core   +5 more sources

Biologically active recombinant human erythropoietin expressed in hairy root cultures and regenerated plantlets of Nicotiana tabacum L. [PDF]

PLoS ONE, 2017
Hairy root culture is a potential alternative to conventional mammalian cell culture to produce recombinant proteins due to its ease in protein recovery, low costs and absence of potentially human pathogenic contaminants.
Poornima Devi Gurusamy, Holger Schäfer, Siva Ramamoorthy, Michael Wink   +3 more
doaj   +3 more sources

Assessment of hairy roots induction in Echium khuzistanicum by different strains of Agrobacterium rhizogenes [PDF]

مجله بیوتکنولوژی کشاورزی, 2020
Objective In this study for the first time, different strains of Agrobacterium rhizogenes, co-cultivation times, and concentrations of acetosyringon in leaf explants of Echium khuzistanicum were examined to produce hairy roots.
samira mohammadi, Raheem Haddad, Vahid Mohammadi   +2 more
doaj   +1 more source