Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity [PDF]
Scientific Reports, 2023 The utilization of nanotechnology and biotechnology for enhancing the synthesis of plant bioactive chemicals is becoming increasingly common. The hairy root culture technique can be used to increase secondary metabolites such as tropane alkaloids ...
Aisha M. Abdelkawy +10 more
doaj +3 more sources
Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants [PDF]
Frontiers in Plant Science, 2023 Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants.
Diptesh Biswas +3 more
doaj +3 more sources
Rhizobium rhizogenes-mediated hairy root induction and plant regeneration for bioengineering citrus. [PDF]
Plant Biotechnol J, 2023 Plant transformation is widely used in functional genetics and bioengineering (BE)-based crop improvement. With the advent and deployment of CRISPR-based genome editing technology and increased public awareness of the safety of BE crops, the applications
Ramasamy M +5 more
europepmc +2 more sources
Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation [PDF]
Plants, 2021 CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy root transformation is a rapid and convenient approach for obtaining transgenic roots. When combined, these techniques represent a fast and effective means of studying gene function.
Alexey S. Kiryushkin +4 more
openaire +4 more sources
Plant Methods, 2021 Background Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology.
Yuanyuan Cheng +5 more
doaj +2 more sources
Optimization of in vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation of soybean for visual screening of transformants using RUBY. [PDF]
Front Plant Sci, 2023 In vitro and ex vitro Agrobacterium rhizogenes-mediated hairy root transformation (HRT) assays are key components of the plant biotechnology and functional genomics toolkit.
Niazian M +4 more
europepmc +2 more sources
An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion. [PDF]
Plant Biotechnol JSummary Genetic transformation is a critical tool for gene editing and genetic improvement of plants. Although many model plants and crops can be genetically manipulated, genetic transformation systems for fruit trees are either lacking or perform poorly.
Liu L +13 more
europepmc +2 more sources
Plant Methods, 2021 Background Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable transformation.
Yaqi Qin +6 more
doaj +2 more sources
Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2012 Transgenic hairy root system is a promising source of secondary metabolites in medicinal plants with high pharmaceutical value.For the first time, hairy roots were established in different explants of Linum mucronatum, an anti-cancer agent producing ...
Afsaneh SAMADI +4 more
doaj +3 more sources
Injection-based hairy root induction and plant regeneration techniques in Brassicaceae [PDF]
Plant MethodsBackground Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roots can be facilitated by employing
Veronika Jedličková +6 more
doaj +2 more sources