Results 31 to 40 of about 15,589 (303)

Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

Plant Methods, 2022
Background Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes.
Jia-Wen Li, Tuo Zeng, Zhi-Zhuo Xu, Jin-Jin Li, Hao Hu, Qin Yu, Li Zhou, Ri-Ru Zheng, Jing Luo, Cai-Yun Wang   +9 more
doaj   +1 more source

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Acta Agriculturae Slovenica, 2016
Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A.
Elnaz NOUROZI, Bahman HOSSEINI, Abbas HASSANI   +2 more
doaj   +1 more source

Transcriptomic Analysis Reveals That Reactive Oxygen Species and Genes Encoding Lipid Transfer Protein Are Associated with Tobacco Hairy Root Growth and Branch Development

Molecular Plant-Microbe Interactions, 2014
The hairy root, a specialized plant tissue that emerges from a cell transformed with transfer DNA (T-DNA) from Agrobacterium rhizogenes, can be used to study root biology and utilized in biotechnological applications.
Jung-Hao Wang, Hsiao-Han Lin, Chi-Te Liu, Ta-Chung Lin, Li-yu Daisy Liu, Kung-Ta Lee   +5 more
doaj   +1 more source

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry

Plant Methods, 2023
Background The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family.
Huiqing Yan, Dandan Ma, Peipei Yi, Guilian Sun, Xingyan Chen, Yin Yi, Xiaolong Huang   +6 more
doaj   +1 more source

Impact of different culture media on hairy roots growth of Valeriana officinalis L.

Acta Agriculturae Slovenica, 2014
Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V.
Ali PAKDIN PARIZI, Mohammad FARSI, Ghorban-Ali NEMATZADEH, Amin MIRSHAMSI   +3 more
doaj   +1 more source

Optimization of hairy root induction conditions in radish (Raphanus sativus L.) and evaluation of biosynthesis ability of secondary metabolites in hairy root cultures [PDF]

مجله بیوتکنولوژی کشاورزی, 2019
Objective Agrobacterium rhizogenes results in the formation of hairy root phenotype in plants. The hairy roots have high growth rate and genetic stability and able to produce plant metabolites.
Zahra Gholami, Nadali Babaeiyan, Ali Pakdin-Parizi, Mahdi Dadmehr   +3 more
doaj   +1 more source

Hairy roots are more sensitive to auxin than normal roots [PDF]

Proceedings of the National Academy of Sciences, 1988
Responses to auxin of Lotus corniculatus root tips or protoplasts transformed by Agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. Three different types of experiments were performed, involving long-term, medium-term, or short-term responses ...
Shen, W.H., Petit, A., Guern, J., Tempe, J.   +3 more
openaire   +3 more sources

Expression of farnesyl diphosphate synthase gene (fps) in Nicotinan tabacum hairy root via Ri-mediated transformation

浙江大学学报. 农业与生命科学版, 2007
To improve phytoalexin synthesis capability of hairy roots, farnesyl diphosphate synthase gene (fps) of Mentha spicata L. was transferred into tobacco via Ri-mediated transformation.
CUI Hong, MU Ping-li, LI Xue-jun, LIU Meng-lin, WANG Zhe   +4 more
doaj   +1 more source

Efficient genetic transformation of Impatiens hawkerii Bull. (Balsamiaceae) using agrobacterium rhizogenes [PDF]

Archives of Biological Sciences, 2009
Transformation of Impatiens hawkerii Bull. mediated by Agrobacterium rhizogenes strain A4M70GUS was studied. Hairy roots developed 10 days after inoculation were excised from the shoot explants and transferred onto Murashige and Skoog's (MS) basal medium
Milošević Snežana, Subotić Angelina, Cingel A., Jevremović Slađana, Ninković Slavica   +4 more
doaj   +1 more source

Effects of Carbohydrates on Rosmarinic Acid Production and In Vitro Antimicrobial Activities in Hairy Root Cultures of Agastache rugosa

Plants, 2023
Agastache rugosa (popularly known as Korean mint) belongs to the Lamiaceae family and comprises 22 species of perennial aromatic medicinal species native to East Asian countries, such as Korea, Taiwan, Japan, and China. A.
Hyeon Ji Yeo, Min Jae Kwon, Sang Yeon Han, Jae Cheol Jeong, Cha Young Kim, Sang Un Park, Chang Ha Park   +6 more
doaj   +1 more source