Results 51 to 60 of about 50,477 (230)
Transport of camptothecin in hairy roots of Ophiorrhiza pumila [PDF]
We have investigated the subcellular accumulation and transport of camptothecin (CPT), a monoterpene indole alkaloid, in hairy roots of Ophiorrhiza pumila. This hairy root produces high amounts of CPT and excretes it into the culture medium. When the hairy roots were exposed to UV radiation, autofluorescence emitted from CPT showed subcellular ...
Mami Yamazaki+4 more
openaire +3 more sources
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas+3 more
core +2 more sources
High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata [PDF]
In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts.
Geelen, Danny, Lambert, Ellen
core +1 more source
Background Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable transformation.
Yaqi Qin+6 more
doaj +1 more source
Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis 1[OPEN] [PDF]
Nuclear movement is involved in cellular and developmental processes across eukaryotic life, often driven by Linker of Nucleoskeleton and Cytoskeleton (LINC) complexes, which bridge the nuclear envelope (NE) via the interaction of Klarsicht/ ANC-1/Syne-1
Cerro Sánchez, Pablo del+3 more
core +1 more source
Hairy root induction from hypocotyl segments of groundnut (Arachis hypogaea L.) [PDF]
Hairy roots were induced from hypocotyl explants excised from seven day old aseptically grown seedlings of groundnut using Agrobacterium rhizogenes 15834. The percentage of hairy root induction and number of hairy roots per ex-plant varied with infection
Karthikeyan, A+3 more
core +2 more sources
Hairy Root Transformation in Lotus japonicus
In L. japonicus, hairy root transformation is the very useful technique to generate transformed root systems in a short term. This protocol was previously described (Kumagai and Kouchi, 2003) with some modifications. After the infection of Agrobacterium rhizogenes, L. japonicus develops not only transformed but also untransformed roots.
Satoru Okamoto+3 more
openaire +3 more sources
Plant cell and organ cultures as a source of phytochemicals [PDF]
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/67430Plant cell and organ cultures constitute a promising platform for the production of numerous valuable secondary compounds.
Bonfill Baldrich, Ma. Mercedes+3 more
core
Application of recombinant DNA technology to studies on plant secondary metabolism [PDF]
This review which is concerned with the application of recombinant DNA technology to studies on plant secondary metabolism, presents the more common plant transformation strategies and shows how these genetic approaches are being used in attempts to ...
Yeoman, Michael M., Zárate, Rafael
core +1 more source
Improving galegine production in transformed hairy roots of Galega officinalis L. via elicitation
Key points This is the first report on hairy root induction and elicitation in G. officinalis. Hairy root cultures can be a potential source for galegine production. Ultrasonic wave was more effective in increasing galegine content.
Maryam Khezri+3 more
doaj +1 more source