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Chemicals from Roots, Hairy Roots, and Their Application
1999Plants produce thousands of different compounds through the secondary metabolism pathways. Since many of these products are obtained by direct extraction from plants that are cultivated in the field or some times even collected in their original habitat several factors can alter their yield.
Blondy Canto-Canché +1 more
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‘Hairy’ cells: where are the roots of this leukemia?
Leukemia & Lymphoma, 2011, December 2011; 52(12): 2205–2206© 2011 Informa UK, Ltd.ISSN: 1042-8194 print / 1029-2403 onlineDOI: 10.3109/10428194.2011.593277 Correspondence: Dr Gunnar Juliusson, Department of Hematology, Skane University Hospital, Lund University BMC B10, Lund, 22184 Sweden.
Gunnar Juliusson +2 more
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Polyprenols in hairy roots of Coluria geoides
Biochemical Society Transactions, 2000Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75%
O. Olszowska +5 more
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Biotechnology Letters, 2013
A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were ...
Katiba Mezreb +5 more
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A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were ...
Katiba Mezreb +5 more
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Plant Molecular Biology, 2005
Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Yasuo Niwa +5 more
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Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Yasuo Niwa +5 more
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Progress and Prospects of Hairy Root Research
2018Nature’s own genetic engineer Agrobacterium rhizogenes was discovered more than 40 years ago, and an increasing number of publications on the use of hairy roots in biotechnology have been published since – with more than 85% of all the publications during the past 15 years. Hairy roots have been successfully exploited in various fields in biotechnology,
Suvi T. Häkkinen +1 more
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Science in China Series C: Life Sciences, 2006
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Xingzhi Wang +4 more
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Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Xingzhi Wang +4 more
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Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens
Biotechnology & Bioengineering, 2000Hairy roots were used to investigate cadmium uptake by Thlaspi caerulescens, a metal hyperaccumulator plant with potential applications in phytoremediation and phytomining. Experiments were carried out in nutrient media under conditions supporting root growth.
Tatjana V. Nedelkoska, Pauline M. Doran
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Establishment of hairy root cultures of Psoralea species
Plant Cell Reports, 1992Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours.
Nguyen, Christophe +3 more
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Chinese Journal of Biotechnology, 2006
The effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root cultures of Polygonum multiflorum Thunb. were studied. The results showed that the 2,4-D, NAA and 6-BA all have obvious effects on the growth of hairy root cultures and the biosynthesis of anthraquinones.
Na Ma, Rong-Min Yu, Chunyan Yan, Yu Zhao
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The effects of exogenous phytohormones on hairy root growth and biosynthesis of anthraquinones in the hairy root cultures of Polygonum multiflorum Thunb. were studied. The results showed that the 2,4-D, NAA and 6-BA all have obvious effects on the growth of hairy root cultures and the biosynthesis of anthraquinones.
Na Ma, Rong-Min Yu, Chunyan Yan, Yu Zhao
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