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Hairy Roots as a Source of Tropane Alkaloids [PDF]

, 2020
The hairy roots developed after Agrobacterium rhizogenes transformation can be used for the production of secondary metabolites which show medicinal properties. Plant secondary metabolites include various classes of chemical compounds, such as the tropane alkaloids found in some members of the Solanaceae family, for example, Atropa belladonna, Datura ...
Przemysław Sitarek, Ewa Skała
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Production of monoclonal antibodies by tobacco hairy roots

Biotechnology and Bioengineering, 1997
Hairy roots of tobacco (Nicotiana tabacum) were used to produce full-length murine lgG(1) monoclonal antibody. The presence of heavy (gamma) and light (kappa) chains and fully assembled antibody was verified by Western blot analysis of root extracts. Antibody levels in the biomass and medium were quantified by ELISA based on detection of gamma-kappa ...
Pauline M. Doran, Raviwan Wongsamuth
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Large-Scale Production of Hairy Root

2004
Many products of interest are synthesized in organized tissues, but not formed in suspension or callus culture. Therefore, most attention has been focused on root cultures. The transgenic plant,"hairy root", has brought us to dramatic improvements in growth rate and high content of desirable products.
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Cryopreservation of Transformed (Hairy) Roots ofArtemisia annua

Cryobiology, 1996
The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection.
Pamela J. Weathers, K.H. Teoh, Douglas B. Walcerz, R.D. Cheetham   +3 more
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Polyacetylenes in Hairy Roots of aPanaxHybrid

Planta Medica, 2003
From the hairy roots of an interspecific hybrid ginseng ( Panax ginseng x P. quinquefolium) known as Pgq, three polyacetylenes were isolated: panaxynol, panaxydol and 1,8-heptadecadiene-3,10-diol. These compounds isolated from the hairy roots were used for quantitative analysis to investigate the polyacetylene production of the hairy roots cultured in ...
Daisuke Washida, Koichiro Shimomura, Susumu Kitanaka   +2 more
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Hairy Root Cultures for Secondary Metabolites Production

2010
Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability.
PISTELLI, LAURA, Giovannini, A., Ruffoni, B., BERTOLI, ALESSANDRA, PISTELLI, LUISA   +4 more
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The host range of infectious hairy-root

The Botanical Review, 1981
According to the literature, the distinctive symptom of the hairy-root disease is the formation of a mass of adventitious roots on the stem of the host plant. Hairy-root was divided into four diseases: infectious hairy-root, non-infectious hairy-root, crown gall and wound overgrowth.
Jozef De Ley, Marcel De Cleene
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Polyacetylenes in Lobelia sessilifolia hairy roots

Phytochemistry, 1994
Lobelia sessilifolia hairy roots (six clones: A-1 ∼4, and J-1 and 2), induced by Agrobacterium rhizogenes ATCC 15834 and A. rhizogenes MAFF 03-01724, grew well and produced some polyacetylenes (lobetyol, lobetyolin and lobetyolinin) in various hormone-free media. J-1 clone, transformed with A.
Koichiro Shimomura, Hiroko Arakawa, Kanji Ishimaru, Michiko Yamanaka   +3 more
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Hernandulcin in hairy root cultures of Lippia dulcis

Plant Cell Reports, 1991
The hairy root culture of Lippia dulcis Trev., Verbenaceae, was established by transformation with Agrobacterium rhizogenes A4. The transformed roots grew well in Murashige and Skoog medium containing 2% sucrose. The roots turned light green when they were cultured under 16 h/day light.
Martina Sauerwein, Koichiro Shimomura, Takeshi Yamazaki   +2 more
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Polyacetylenes in hairy roots of Platycodon grandiflorum

Phytochemistry, 1996
Abstract Hairy roots of Korean balloon flower (Platycodon grandiflorum) were induced from root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene (lobetyol, lobetyolin and lobetyolinin) production of 10 hairy roots clones cultured in 1 4 Gamborg B5 (B5) liquid medium were determined.
Hiromi Tada, Jun Cheul Ahn, Baik Hwang, Koichiro Shimomura, Kanji Ishimaru, Kazuo Sasaki   +5 more
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