Results 321 to 330 of about 607,179 (386)

Hairy Roots

2021
Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants.
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Hairy Root-activation Tagging: a High-throughput System for Activation Tagging in Transformed Hairy Roots

Plant Molecular Biology, 2005
Activation tagging is a powerful technique for generating gain-of-function mutants in plants. We developed a new vector system for activation tagging of genes in "transformed hairy roots". The binary vector pHR-AT (Hairy Root-Activation Tagging) and its derivative pHR-AT-GFP contain a cluster of rol (rooting locus) genes together with the right border ...
Hikaru, Seki, Tomoko, Nishizawa, Nobukazu, Tanaka, Yasuo, Niwa, Shigeo, Yoshida, Toshiya, Muranaka   +5 more
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Chemicals from Roots, Hairy Roots, and Their Application

1999
Plants produce thousands of different compounds through the secondary metabolism pathways. Since many of these products are obtained by direct extraction from plants that are cultivated in the field or some times even collected in their original habitat several factors can alter their yield.
B, Canto-Canché, V M, Loyola-Vargas
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Polyprenols in hairy roots of Coluria geoides

Biochemical Society Transactions, 2000
Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75%
K, Skorupińska-Tudek, V S, Hung, O, Olszowska, M, Furmanowa, T, Chojnacki, E, Swiezewska   +5 more
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Overexpressing chalcone synthase (CHS) gene enhanced flavonoids accumulation in Glycyrrhiza uralensis hairy roots

Botany Letters, 2019
Flavonoids are key bioactive ingredients in Glycyrrhizia uralensis, a widely used herbal medicine in China. Chalcone synthase (CHS) is the first rate-limiting enzyme involved in the flavonoid biosynthetic pathway.
Yan Yin, Jiaming Hou, Shaokai Tian, Lin Yang, Zhi-xin Zhang, Wen-dong Li, Y. Liu   +6 more
semanticscholar   +1 more source

Flavonoids from Glycyrrhiza pallidiflora hairy root cultures

Phytochemistry, 2001
Glycyrrhiza pallidiflora hairy roots were induced from axenic young plants by direct infection with Agrobacterium rhizogenes. The chemical constituents were then investigated after mass culture. The isoflavone, licoagroisoflavone and the coumestan, licoagroside C, were isolated along with seven known flavonoids.
Wei, Li, Kazuo, Koike, Yoshihisa, Asada, Masao, Hirotani, Hekai, Rui, Takafumi, Yoshikawa, Tamotsu, Nikaido   +6 more
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Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro

2003
The bacterial genus Agrobucterium includes two species of considerable interest to plant physiologists and pathologists alike. Infection by virulent strains of A. tumefaciens induces the formation of tumors and infection by A. rhizogenes the proliferation of roots in a wide range of dicotyledenous plants: crown-gall and hairy root diseases are not ...
C S, Hunter, S J, Neill
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Dynamic flux cartography of hairy roots primary metabolism

Phytochemistry, 2007
A dynamic model for plant cell and hairy root primary metabolism is presented. The model includes nutrient uptake (Pi, sugars, nitrogen sources), the glycolysis and pentose phosphate pathways, the TCA cycle, amino acid biosynthesis, respiratory chain, biosynthesis of cell building blocks (structural hexoses, organic acids, lipids, and organic ...
M, Cloutier, M, Perrier, M, Jolicoeur
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Cryopreservation of Transformed (Hairy) Roots ofArtemisia annua

Cryobiology, 1996
The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection.
K H, Teoh, P J, Weathers, R D, Cheetham, D B, Walcerz   +3 more
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Induction of hairy roots in Gmelina arborea Roxb. and production of verbascoside in hairy roots

Plant Science, 2005
Seedling tissues of Gmelina arborea, a medicinally important tree species, were infected with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 32% of the explants. Transgenic status of the roots was confirmed by PCR using rolB specific primers, and subsequently, by Southern analysis of the PCR ...
Shrutika Dhakulkar, T.R. Ganapathi, Sujata Bhargava, V.A. Bapat   +3 more
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