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Hairy Root Cultures for Secondary Metabolites Production
2010Hairy roots (HRs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen causes the HR disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability.
PISTELLI, LAURA +4 more
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Hairy root transformation in alfalfa (Medicago sativa L.)
Theoretical and Applied Genetics, 1987The widely cultivated forage legume alfalfa (Medicago sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype.
Spanò L. +4 more
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Journal of Agricultural and Food Chemistry, 2019
Glucosinolates are secondary metabolites that play important roles in plant defense and human health, as their production in plants is enhanced by overexpressing transcription factors.
D. M. Cuong +5 more
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Glucosinolates are secondary metabolites that play important roles in plant defense and human health, as their production in plants is enhanced by overexpressing transcription factors.
D. M. Cuong +5 more
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Biotechnology Letters, 2013
A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were ...
Yoann, Huet +5 more
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A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were ...
Yoann, Huet +5 more
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Science in China Series C: Life Sciences, 2006
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Hongwei, Xu +4 more
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Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA.
Hongwei, Xu +4 more
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Large-Scale Production of Hairy Root
2004Many products of interest are synthesized in organized tissues, but not formed in suspension or callus culture. Therefore, most attention has been focused on root cultures. The transgenic plant,"hairy root", has brought us to dramatic improvements in growth rate and high content of desirable products.
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Flavonoid content and antioxidant activity of Artemisia vulgaris L. “hairy” roots
Preparative Biochemistry & Biotechnology, 2019We investigated the effect of Agrobacterium rhizogenes-mediated transformation on antioxidant activity of Artemisia vulgaris “hairy” roots. It appeared that transformation may increase flavonoid content as well as DPPH-scavenging activity and ability to ...
N. Matvieieva +7 more
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Polyacetylenes in Lobelia sessilifolia hairy roots
Phytochemistry, 1994Lobelia sessilifolia hairy roots (six clones: A-1 ∼4, and J-1 and 2), induced by Agrobacterium rhizogenes ATCC 15834 and A. rhizogenes MAFF 03-01724, grew well and produced some polyacetylenes (lobetyol, lobetyolin and lobetyolinin) in various hormone-free media. J-1 clone, transformed with A.
Kanji Ishimaru +3 more
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2012
The invention of hairy root induction from various higher plants has provided a new set of hopes for using such organs in vitro as an alternative to cell cultures, chiefly because of their genetic stabilities that impart them high biochemical consistencies.
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The invention of hairy root induction from various higher plants has provided a new set of hopes for using such organs in vitro as an alternative to cell cultures, chiefly because of their genetic stabilities that impart them high biochemical consistencies.
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Biotechnology of Hairy Root Systems
2013Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism.- Perspectives of the Metabolic Engineering of Terpenoid Indole Alkaloids in Catharanthus roseus Hairy Roots.- Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy ...
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