Results 41 to 50 of about 12,296 (223)

Efficient transfection of the archaebacterium Halobacterium halobium [PDF]

open access: yesJournal of Bacteriology, 1987
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA.
S W, Cline, W F, Doolittle
openaire   +2 more sources

Expression of microbial rhodopsins in Escherichia coli and their extraction and purification using styrene-maleic acid copolymers

open access: yesSTAR Protocols, 2022
Summary: Microbial rhodopsins are photoreceptive membrane proteins showing various light-dependent biological activities. Styrene-maleic acid (SMA) copolymers spontaneously form nanoscale lipid particles containing membrane proteins and associated lipids
Keiichi Kojima, Yuki Sudo
doaj   +1 more source

A transcription factor links growth rate and metabolism in the hypersaline adapted archaeon Halobacterium salinarum

open access: yesMolecular Microbiology, 2014
Co‐ordinating metabolism and growth is a key challenge for all organisms. Despite fluctuating environments, cells must produce the same metabolic outputs to thrive. The mechanisms underlying this ‘growth homeostasis’ are known in bacteria and eukaryotes,
Horia Todor   +5 more
semanticscholar   +1 more source

Transcription start site associated RNAs (TSSaRNAs) are ubiquitous in all domains of life.

open access: yesPLoS ONE, 2014
A plethora of non-coding RNAs has been discovered using high-resolution transcriptomics tools, indicating that transcriptional and post-transcriptional regulation is much more complex than previously appreciated.
Livia S Zaramela   +4 more
doaj   +1 more source

Regulated polyploidy in halophilic archaea [PDF]

open access: yes, 2006
Polyploidy is common in higher eukaryotes, especially in plants, but it is generally assumed that most prokaryotes contain a single copy of a circular chromosome and are therefore monoploid.
Jörg Soppa   +7 more
core   +1 more source

The role of squalene in the organization of monolayers derived from lipid extracts of Halobacterium salinarum

open access: yesLangmuir, 2013
We have studied interfacial compressibility and lateral organization in monolayer configurations of total (squalene containing) and polar (squalene-devoid) lipid extracts of Halobacterium salinarum NRC-1, an extremely halophilic archaeon.
Sean F Gilmore   +5 more
semanticscholar   +1 more source

Current strategies to enhance the target efficiency of advanced multifunctional ultrasound contrast agents in ischemic diseases

open access: yesInterdisciplinary Medicine, EarlyView.
The graphical abstract outlines a novel multifunctional ultrasound contrast agent for the diagnosis and treatment of ischemic diseases, along with innovative strategies to enhance its targeting efficiency. Abstract Ischemic diseases, such as myocardial infarction, stroke, and peripheral ischemia, are leading causes of death and disability worldwide ...
Yangyang Zhou   +3 more
wiley   +1 more source

Biological properties of carotenoids extracted from Halobacterium halobium isolated from a Tunisian solar saltern

open access: yesBMC Complementary and Alternative Medicine, 2013
BackgroundBioactive molecules have received increasing attention due to their nutraceutical attributes and anticancer, antioxidant, antiproliferative and apoptosis-inducing properties.
Molka Abbes   +6 more
semanticscholar   +1 more source

Halobacterium cutirubrum tRNA sequences [PDF]

open access: yesFEBS Letters, 1985
The primary structures of nine Halobacterium cutirubrum tRNAs are presented. These tRNAs are compared with other archaebacterial tRNAs and in particular with their H. volcanii counterparts. Striking similarities are observed among isoacceptor tRNA species; observed differences between a given tRNA pair of the two halophiles having the same anticodon ...
Nicoghosian, K.   +2 more
openaire   +1 more source

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

open access: yesThe FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami   +6 more
wiley   +1 more source

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