Results 71 to 80 of about 12,018 (199)

Efficient transfection of the archaebacterium Halobacterium halobium [PDF]

Journal of Bacteriology, 1987
We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA.
S W, Cline, W F, Doolittle
openaire   +2 more sources

Writing the Engram: Epigenetic Mechanisms of Memory Allocation

Journal of Neurochemistry, Volume 169, Issue 12, December 2025.
Memory allocation refers to the selective recruitment of neurons to engram cells upon memory formation. While previous studies have documented the importance of intrinsic excitability and the transcription factor CREB on memory allocation, epigenetic mechanisms have remained poorly understood. Here, we summarise recent discoveries epigenetic mechanisms
Isabella Tarulli, Rebecca Toscano‐Rivalta, Lisa Watt, Johannes Gräff   +3 more
wiley   +1 more source

Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing

BMC Genomics, 2006
Background Whole genome amplification is an increasingly common technique through which minute amounts of DNA can be multiplied to generate quantities suitable for genetic testing and analysis.
Plant Ramona N, Tartaro Karrie R, Gerstein Mark B, Sarkis Gary J, de Winter Alex, Pinard Robert, Egholm Michael, Rothberg Jonathan M, Leamon John H   +8 more
doaj   +1 more source

Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

Frontiers in Microbiology, 2020
Halobacterium sp. NRC-1 (NRC-1) is an extremely halophilic archaeon that is adapted to multiple stressors such as UV, ionizing radiation and arsenic exposure; it is considered a model organism for the feasibility of microbial life in iron-rich brine on ...
Karina S. Kunka, Karina S. Kunka, Jessie M. Griffith, Jessie M. Griffith, Chase Holdener, Katarina M. Bischof, Haofan Li, Priya DasSarma, Shiladitya DasSarma, Joan L. Slonczewski   +9 more
doaj   +1 more source

Nano‐Infrared Imaging and Spectroscopy of Animal Cells in Liquid Environment

Small, Volume 21, Issue 47, November 27, 2025.
The use of SiC membranes as a stable, biocompatible interface between cells in their medium and the scanning probe of an infrared scattering‐type scanning near field optical microscope is shown to yield nano‐IR images and spectra from living fibroblast cells using a broadband synchrotron IR source. New possibilities for adjustments of probing depth for
Alexander Veber, Cecilia Spedalieri, Janina Kneipp   +2 more
wiley   +1 more source

The glycolipid of Halobacterium sodomense [PDF]

Journal of General Microbiology, 1990
SUMMARY: The glycolipid of the halophilic archaeobacterium Halobacterium sodomense has been analysed by spectroscopic methods including 13C NMR to establish structural details, and has been shown to be a sulphated C20,C20 mannosyl glucosyl diether glycerol.
A Trincone, B Nicolaus, L Lama, M De Rosa, A Gambacorta, WD Grant   +5 more
openaire   +4 more sources

Ultrasound‐Mediated Biotransfection of Engineered Bone Marrow Mesenchymal Stem Cells in Treated Bone Defects through Intracellular Cavitation

Advanced Science, Volume 12, Issue 40, October 27, 2025.
Ultrasound‐mediated biotransfection through intracellular cavitation increases plasmid nuclear translocation and gene transfection efficiency by 284.7% and 131.6% in engineered bone marrow mesenchymal stem cells (BMSCs) compared to traditional ultrasound‐targeted microbubble destruction techniques. The maintained bone morphogenetic protein 2 expression
Zhili Xu, Huijuan Xin, Yu Wang, Renhao Xu, Yanni He, Meijun Zhou, Zhengqiang Yuan, Hongmei Liu   +7 more
wiley   +1 more source

Biosynthesis of 2H-labeled Photochrome Trans-membrane Protein Bacteriorhodopsin by Halobacterium Halobacterium Halobium

European Reviews of Chemical Research, 2014
The semi-preparative biosynthesis of photochrome trans-membrane protein bacteriorhodopsin (output 810 mg), labeled with deuterium on functionally important amino acid residues  [2,3,4,5,6- 2 H5]phenylalanine, [3,5- 2 H2]tyrosine, and [2,4,5,6,7- 2 H5]tryptophan was carried out with using a photo-organotrophic halobacterium Halobacterium halobium. The
Oleg Mosin, Ignat Ignatov
openaire   +2 more sources

Physio-chemical and biological properties of partially purified exopolymers from newly isolated halophilic bacterial strain SM5 [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2007
An extremely halophilic bacterial strain SM5 producing exopolymers was isolated from Thai traditional fermented fish (Pla-ra). Morphological and biochemical properties and 16S rDNA sequencing revealed that the strain was Halobacterium sp. SM5.
Patcharee Lungmann, Wanna Choorit, Poonsuk Prasertsan   +2 more
doaj  

Synthesis of sulfate esters of phosphatidylglycerol (diphytanyl ether analog)

Journal of Lipid Research, 1973
Synthesis of 1-sn-phosphatidyl-3′-sn-glycero-1′-sulfate (phosphatidylglycero-1-sulfate) was achieved by monosulfation of 1-sn-phosphatidyl-3′-sn-glycerol (diphytanyl ether analog) with an equimolar amount of SO3–pyridine complex at room temperature; with
A.J. Hancock, M. Kates
doaj   +1 more source