Results 151 to 160 of about 5,644 (180)

The membrane proteome of Halobacterium salinarum

PROTEOMICS, 2005
AbstractThe identification of 114 integral membrane proteins from Halobacterium salinarum was achieved using liquid chromatography/tandem mass spectrometric (LC/MS/MS) techniques, representing 20% of the predicted alpha‐helical transmembrane proteins of the genome.
Klein, C., Garcia-Rizo, C., Bisle, B., Scheffer, B., Zischka, H., Pfeiffer, F., Siedler, F., Oesterhelt, D.   +7 more
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Iron-uptake in the Euryarchaeon Halobacterium salinarum

BioMetals, 2007
Iron-uptake is well studied in a plethora of pro- and eukaryotic organisms with the exception of Archaea, which thrive mainly in extreme environments. In this study, the mechanism of iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum strain JW 5 was analyzed.
Dirk, Hubmacher, Berthold F, Matzanke, Stefan, Anemüller   +2 more
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Repair of UV damage in Halobacterium salinarum

Biochemical Society Transactions, 2003
Halobacterium is one of the few known Archaea that tolerates high levels of sunlight in its natural environment. Photoreactivation is probably its most important strategy for surviving UV irradiation and we have shown that both of the major UV photoproducts, cyclobutane pyrimidine dimers (CPDs) and (6–4) photoproducts, can be very efficiently repaired ...
S, McCready, L, Marcello
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Investigations of iron uptake in Halobacterium salinarum

Biochemical Society Transactions, 2002
The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake of 55Fe and [14C]citrate gave evidence only for the accumulation of iron.
D, Hubmacher, B F, Matzanke, S, Anemüller   +2 more
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Isotopic Labeling of Proteins in Halobacterium salinarum

2015
It is often necessary to obtain isotopically labeled proteins containing (15)N, (13)C, or (2)H for nuclear magnetic resonance; and (2)H for small-angle neutron scattering or neutron diffraction studies. To achieve uniform isotopic labeling, protein expression is most commonly performed in Escherichia coli or yeast using labeled media. However, proteins
Thomas E, Cleveland, Zvi, Kelman
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The Low Molecular Weight Proteome of Halobacterium salinarum

Journal of Proteome Research, 2007
Systematic investigation of low molecular weight proteins (LMW, below 20 kDa) in the archaeon Halobacterium salinarum resulted in a 6-fold enhancement of the identification rate, reaching 35% of the theoretical proteome in that size range. This was achieved by optimization of common protocols for protein analysis with general applicability.
Klein, C., Aivaliotis, M., Olsen, J., Falb, M., Besir, H., Scheffer, B., Bisle, B., Tebbe, A., Konstantinidis, K., Siedler, F., Pfeiffer, F., Mann, M., Oesterhelt, D.   +12 more
openaire   +3 more sources

Reconstruction, modeling & analysis of Halobacterium salinarum R-1 metabolism

Mol. BioSyst., 2008
We present a genome-scale metabolic reconstruction for the extreme halophile Halobacterium salinarum. The reconstruction represents a summary of the knowledge regarding the organism's metabolism, and has already led to new research directions and improved the existing annotation.
Gonzalez, O., Gronau, S., Falb, M., Pfeiffer, F., Mendoza, E., Zimmer, R., Oesterhelt, D.   +6 more
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High production of bacteriorhodopsin from wild type Halobacterium salinarum

Extremophiles, 2015
Bacteriorhodopsin (bR) is a trans-membrane proton pump found in the purple membrane of Halobacterium salinarum. This protein has high photochemical and photoelectric conversion efficiency and thermal stability, allowing it to withstand high temperatures, high salinity, and nutritionally-limited environments. The ability of this protein to convert light
Mansooreh-Sadat, Seyedkarimi, Asieh, Aramvash, Rohollah, Ramezani   +2 more
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Proteomanalysen an Halobacterium salinarum

2005
Im Rahmen dieser Arbeit wurden verschiedene Proteome von H. salinarum untersucht, die nach zellulären Kompartimenten unterschieden wurden in (1) das Flagellarmotor-Proteom (2) das Cytosolproteom und (3) das Membranproteom. Die Untersuchung des Flagellarmotors erfolgte hauptsächlich auf struktureller Basis mittels Elektronenmikroskopie.
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Effects of divalent cations on Halobacterium salinarum cell aggregation

Journal of Bioscience and Bioengineering, 2007
Ca(2+) was found to be essential for initiating Halobacterium salinarum CCM 2090 cell aggregation. The floc formed from such aggregation could easily be dissociated without cellular lysis by sodium citrate. Cr(2+), Mn(2+), Fe(3+), Co(2+), Ni(2+), Cu(2+), and Zn(2+) could replace Ca(2+). However, Mg(2+), Sr(2+), Mo(2+), Cd(2+), Sn(2+), Hg(2+), and Pb(2+)
Yoshitaka, Kawakami, Nobuhiro, Hayashi, Mizue, Ema, Masashi, Nakayama   +3 more
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