Identification of the D-glucuronyl C5-epimerase that introduces iduronic acid into N-linked glycans decorating archaeal glycoproteins [PDF]
Communications BiologyIn Halobacterium salinarum, halophilic archaea that grow in saturated salt solutions, glycoproteins are modified by an N-linked tetrasaccharide that includes iduronic acid (IdoA).
Zlata Vershinin +8 more
doaj +2 more sources
Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum. [PDF]
Sci Rep, 2017 AbstractThe retinylidene protein bacteriorhodopsin (BR) is a heptahelical light-dependent proton pump found in the purple membrane of the archaeon Halobacterium salinarum. We now show that when reconstituted into large unilamellar vesicles, purified BR trimers exhibit light-independent lipid scramblase activity, thereby facilitating transbilayer ...
Verchère A +8 more
europepmc +4 more sources
Development of New Modular Genetic Tools for Engineering the Halophilic Archaeon Halobacterium salinarum. [PDF]
PLoS ONE, 2015 Our ability to genetically manipulate living organisms is usually constrained by the efficiency of the genetic tools available for the system of interest.
Rafael Silva-Rocha +4 more
doaj +2 more sources
Assessment of methods for evaluating structural stability of cell envelope fragments in hypersaline brines as biosignatures of ancient microbial life [PDF]
Scientific ReportsThe study of biomolecular stability of proteins and lipids in extreme saline environments is critical for understanding the preservation of potential microbial biosignatures of ancient life on Earth and other planetary bodies, including Mars.
Lucas Bourmancé +3 more
doaj +2 more sources
The Effect of Bacterioruberin Deletion on Production of Bacteriorhodopsin in Halobacterium salinarum R1 [PDF]
Journal of Pure and Applied Chemistry Research, 2018 Bacteriorhodopsin is a retinal protein located in purple membrane of Halobacterium salinarum which acts as light-dependent proton pump. Bacterioruberin is a by-product in bacteriorhodopsin biosynthesis pathway in Halobacterium salinarum.
Abbas Karimi Fard +2 more
doaj +1 more source
Photoresponses of Halobacterium salinarum to Repetitive Pulse Stimuli [PDF]
Biophysical Journal, 1998 Halobacterium salinarum cells from 3-day-old cultures have been stimulated with different patterns of repetitive pulse stimuli. A short train of 0.6-s orange light pulses with a 4-s period resulted in reversal peaks of increasing intensity. The reverse occurred when blue light pulses were delivered as a finite train: with a 3-s period, the response ...
Cercignani G, Lucia S, Petracchi D
openaire +4 more sources
GenomeFingerprinter and universal genome fingerprint analysis for systematic comparative genomics [PDF]
, 2013 How to compare whole genome sequences at large scale has not been achieved via conventional methods based on pair-wisely base-to-base comparison; nevertheless, no attention was paid to handle in-one-sitting a number of genomes crossing genetic category ...
Ai, Hannan +3 more
core +7 more sources
MutS and MutL are dispensable for maintenance of the genomic mutation rate in the halophilic archaeon Halobacterium salinarum NRC-1. [PDF]
PLoS ONE, 2010 BACKGROUND: The genome of the halophilic archaeon Halobacterium salinarum NRC-1 encodes for homologs of MutS and MutL, which are key proteins of a DNA mismatch repair pathway conserved in Bacteria and Eukarya.
Courtney R Busch, Jocelyne DiRuggiero
doaj +1 more source
Haloglomus irregulare gen. nov., sp. nov., a New Halophilic Archaeon Isolated from a Marine Saltern [PDF]
, 2020 A halophilic archaeal strain, designated F16-60T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates.
Durán Viseras, Ana +2 more
core +1 more source
Inference of expanded Lrp-like feast/famine transcription factor targets in a non-model organism using protein structure-based prediction. [PDF]
PLoS ONE, 2014 Widespread microbial genome sequencing presents an opportunity to understand the gene regulatory networks of non-model organisms. This requires knowledge of the binding sites for transcription factors whose DNA-binding properties are unknown or difficult
Justin Ashworth +4 more
doaj +1 more source