Results 121 to 130 of about 2,427 (150)

CRISPR-Cas targeting in Haloferax volcanii promotes within-species gene exchange by triggering homologous recombination

Choudhary DK, Turgeman-Grott I, Robinzon S, Gophna U.   +3 more
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Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from <i>Haloferax volcanii</i> in aqueous ionic liquid solvent mixtures.

RSC Sustain
Schindl A, Hagen ML, Cooley I, Jäger CM, Warden AC, Zelzer M, Allers T, Croft AK.   +7 more
europepmc   +1 more source

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In Vivo Protein Cross-Linking and Coimmunoprecipitation in Haloferax volcanii

Methods in Molecular Biology, 2022
Coimmunoprecipitation is a powerful and commonly used method to identify protein-protein interactions in a physiological context. Here, we report a coimmunoprecipitation protocol that was adapted and optimized for the haloarchaeon Haloferax volcanii to identify interacting partners to the LonB protease.
Rosana Esther De Castro, Micaela Cerletti   +1 more
exaly   +4 more sources

Haloferax volcanii

Trends in Microbiology, 2019
In this infographic we present the main tools available for the halophilic archaeon Haloferax volcanii, which have enabled successful research on its biology, including its genetics, proteostasis, cell surface structures, metabolic pathways, and adaptation to high salt environments. Isolated from the Dead Sea in 1975, Haloferax volcanii thrives in high
Mechthild, Pohlschroder, Stefan, Schulze
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Structural and functional adaptation of Haloferax volcanii TFEα/β [PDF]

Nucleic Acids Research, 2018
The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit.
Konstantinos Thalassinos, Fabian Blombach, Angelo MIGUEL Figueiredo   +2 more
exaly   +5 more sources

Nutrition of the Halophilic Archaebacterium, Haloferax volcanii

Systematic and Applied Microbiology, 1990
Summary A synthetic medium containing glycerol and succinate as carbon sources, NH4Cl as nitrogen source, thiamine and biotin, as well as salts, supports growth of Haloferax volcanii and other halophilic archaebacteria. A number of single carbon compounds can also support growth, as can single nitrogen compounds (urea, histidine and glutamate).
Tiiu Kauri, Rebecca Wallace, Donn J. Kushner   +2 more
openaire   +1 more source

The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii

Extremophiles, 2007
Those aerobic archaea whose genomes have been sequenced possess four adjacent genes that, by sequence comparisons with bacteria and eukarya, appear to encode the component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in the archaea.
Dina M, Al-Mailem, David W, Hough, Michael J, Danson   +2 more
openaire   +2 more sources

Novel glycoproteins of the halophilic archaeon Haloferax volcanii

Archives of Microbiology, 2000
Archaea possess many eukaryote-like properties, including the ability to glycosylate proteins. Using oligosaccharide staining and lectin binding, this study revealed the existence of several glycosylated Haloferax volcanii membrane proteins, besides the previously reported surface layer (S-layer) glycoprotein.
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Small RNAs of the halophilic archaeon Haloferax volcanii

Biochemical Society Transactions, 2009
In recent years, sRNAs (small non-coding RNAs) have been found to be abundant in eukaryotes and bacteria and have been recognized as a novel class of gene expression regulators. In contrast, much less is known about sRNAs in archaea, except for snoRNAs (small nucleolar RNAs) that are involved in the modification of bases in stable RNAs.
Jörg, Soppa, Julia, Straub, Mariam, Brenneis, Angelika, Jellen-Ritter, Ruth, Heyer, Susan, Fischer, Michaela, Granzow, Björn, Voss, Wolfgang R, Hess, Brian, Tjaden, Anita, Marchfelder   +10 more
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Fructose transport byHaloferax volcanii

Canadian Journal of Microbiology, 1995
Uptake of fructose by intact cells of Haloferax volcanii, one of the sugar-utilizing halobacteria, was examined with the following results. (i) The fructose transporter was inducible, (ii) Kinetic analysis showed a Ktof 0.37 μM and a Vmaxof 4.61 nmol∙mg protein−1∙min−1.
Jin-Ichiro Takano, Kouichi Kaidoh, Naoki Kamo   +2 more
openaire   +1 more source