Results 101 to 110 of about 4,256 (186)

DNA replication origins in Haloferax volcanii [PDF]

open access: yes, 2009
DNA replication is fundamental to the proliferation of life. Sites of DNA replication initiation are called replication origins. Bacteria replicate from a single origin whereas eukaryotes utilise multiple origins for each chromosome. The archaeal domain includes species which replicate using multiple origins of replication in addition to those which ...
openaire   +2 more sources

Overexpression and purification of halophilic proteins inHaloferax volcanii [PDF]

open access: yesBioengineered Bugs, 2010
Halophilic enzymes function optimally at high salt concentrations and are active at low water availability. Such conditions are encountered at elevated concentrations of solutes such as salts and sugars, and at high concentrations of organic solvents.
openaire   +2 more sources

Regulation of DNA Topology in Archaea: State of the Art and Perspectives

open access: yesMolecular Microbiology, Volume 123, Issue 3, Page 245-264, March 2025.
DNA topoisomerases are ubiquitous enzymes that play a crucial role in regulating DNA supercoiling which affects fundamental biological processes involving DNA. In this review, we summarize the current understanding of regulation of supercoiling by DNA topoisomerases in the third domain of life, the Archaea, with a particular focus on three key model ...
Paul Villain, Tamara Basta
wiley   +1 more source

Experimental characterization of Cis-acting elements important for translation and transcription in Halophilic Archaea [PDF]

open access: yes, 2007
Summary The basal transcription apparatus of archaea is well characterized. However, much less is known about the mechanisms of transcription termination and translation initation.
Jörg Soppa   +11 more
core   +2 more sources

Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome

open access: yeseLife, 2017
Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength.
Paul E Schavemaker   +2 more
doaj   +1 more source

HPClas: A data‐driven approach for identifying halophilic proteins based on catBoost

open access: yesmLife, Volume 3, Issue 4, Page 515-526, December 2024.
Abstract Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean‐up, and energy production.
Shantong Hu   +7 more
wiley   +1 more source

Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation [PDF]

open access: yes, 2020
Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile Haloferax volcanii mutants from a transposon insertion library.
Mike Dyall-Smith   +17 more
core   +1 more source

Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon

open access: yesScientific Reports, 2020
Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the ...
Andrea M. Makkay   +5 more
doaj   +1 more source

Polyploidy in haloarchaea: advantages for growth and survival

open access: yesFrontiers in Microbiology, 2014
The investigated haloarchaeal species, Halobacterium salinarum, Haloferax mediterranii, and H. volcanii, have all been shown to be polyploid. They contain several replicons that have independent copy number regulation, and most have a higher copy number ...
Karolin eZerulla, Jörg eSoppa
doaj   +1 more source

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