Genetic and biochemical identification of a novel single-stranded DNA-binding complex in Haloferax volcanii [PDF]
, 2012 Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded β-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting and ...
Allers, Thorsten +2 more
core +1 more source
RiboMicrobe: An Integrated Translatome Atlas for Microorganism
Advanced Science, Volume 12, Issue 48, December 29, 2025.RiboMicrobe is a comprehensive database for Ribo‐seq data from prokaryotes, featuring 891 Ribo‐seq, 369 RNA‐seq, and 62 proteome datasets from 38 species. The database not only provides various data visualization results but also includes two sORF prediction models and a suit of bioinformatics tools for comparative analysis, facilitating easy access ...
Yingshun Zhou +12 more
wiley +1 more source
Structural and functional adaptation of Haloferax volcanii TFEα/β [PDF]
Nucleic Acids Research, 2018 The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit.
Blombach, F +9 more
openaire +4 more sources
Protein acetylation in archaea, bacteria, and eukaryotes [PDF]
, 2010 Proteins can be acetylated at the alpha-amino group of the N-terminal amino acid (methionine or the penultimate amino acid after methionine removal) or at the epsilon-amino group of internal lysines.
Lainio, Jarmo +1 more
core +1 more source
Lipid Anchoring of Archaeosortase Substrates and Midcell Growth in Haloarchaea
mBio, 2020 The archaeal cytoplasmic membrane provides an anchor for many surface proteins. Recently, a novel membrane anchoring mechanism involving a peptidase, archaeosortase A (ArtA), and C-terminal lipid attachment of surface proteins was identified in the model
Mohd Farid Abdul-Halim +5 more
doaj +1 more source
Structural features of halophilicity derived from the crystal structure of dihydrofolate reductase from the Dead Sea halophilic archaeon, Haloferax volcanii [PDF]
, 1982 Background: The proteins of halophilic archaea require high salt concentrations both for stability and for activity, whereas they denature at low ionic strength. The structural basis for this phenomenon is not yet well understood.
Pieper, Ursula +3 more
core +5 more sources
Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.
Microbial Biotechnology, Volume 18, Issue 12, December 2025.Genetic manipulation of Halomonas species has been limited by poor transformation efficiencies. Here, we establish a highly efficient electroporation protocol for Halomonas elongata DSM 2581. This method also proved transferable to other Halomonas strains, encouraging further genetic manipulation work in underexplored species.
André A. B. Coimbra +2 more
wiley +1 more source
Dinucleoside Polyphosphates in Cellular Signaling: Function and Evolution Across Life
BioEssays, Volume 47, Issue 10, October 2025.Although discovered more than 50 years ago, the cellular functions of Ap4A and related dinucleotides remain largely enigmatic. To address this knowledge gap, we organized a conference showcasing recent research that highlights the critical role of Ap4A as a global regulator across all domains of life.
Gert Bange +5 more
wiley +1 more source
Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea : research article [PDF]
, 2007 Background Differential expression of genes can be regulated on many different levels. Most global studies of gene regulation concentrate on transcript level regulation, and very few global analyses of differential translational efficiencies exist.
Hammelmann, Mathias +7 more
core
Rings in the Extreme: PCNA Interactions and Adaptations in the Archaea [PDF]
, 2012 Biochemical and structural analysis of archaeal proteins has enabled us to gain great insight into many eukaryotic processes, simultaneously offering fascinating glimpses into the adaptation and evolution of proteins at the extremes of life. The archaeal
Bunting, KA, Winter, JA
core +2 more sources