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Engineered Halomonas spp. for production of l-Lysine and cadaverine

Bioresource Technology, 2022
Cadaverine, a derivative of l-lysine, has been used as a monomer for the synthesis of bio-based nylon-5,6. This study engineered Halomonas bluephagenesis TD1.0 by blocking the feedback inhibition, overexpressing the key l-lysine synthesis genes, strengthening the l-lysine export system and increasing the supply of oxaloacetate for production of l ...
Cuihuan, Zhao   +8 more
openaire   +2 more sources

Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01

Bioresource Technology, 2011
An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56 h fed-batch process.
Dan, Tan   +3 more
openaire   +2 more sources

Analysis of the Genome of the Moderate Halophile Halomonas eurihalina

Current Microbiology, 2002
We have studied the genomic organization of Halomonas eurihalina, a moderately halophilic bacterium that produces an anionic exopolysaccharide with a potentially wide range of applications in industry. To estimate the genome size of strain F2-7, large restriction fragments of genomic DNA were separated by pulsed-field gel electrophoresis.
Inmaculada, Llamas   +5 more
openaire   +2 more sources

Manipulation of polyhydroxyalkanoate granular sizes in Halomonas bluephagenesis

Metabolic Engineering, 2019
Bacterial polyhydroxyalkanoates (PHA) are a family of intracellular polyester granules with sizes ranging from 100 to 500 nm. Due to their small sizes, it has been very difficult to separate the PHA granules from the bacterial broths. This study aims to engineer the PHA size control mechanism to obtain large PHA granular sizes beneficial for the ...
Rui, Shen   +4 more
openaire   +2 more sources

Turgor regulation in a novel Halomonas species

Archives of Microbiology, 1993
The mechanisms by which a novel eubacterium, identified as belonging to the genus Halomonas, adapted to increases in the extracellular osmotic potential were investigated. It was shown that the ability of the bacterium to grow after hyperosmotic shock was dependent on the presence of potassium ions.
Stephen P. Cummings   +2 more
openaire   +1 more source

Halomonas magadii sp. nov., a new member of the genus Halomonas , isolated from a soda lake of the East African Rift Valley

Extremophiles, 2000
A number of novel alkaliphilic organotrophic bacteria have been isolated from several saline and alkaline East African soda lakes. The new isolates grow at pH values between 7.0 and 11.0, with pH optima for growth between 9.0 and 10.0. Growth occurs at total salts concentration between 0% and 20% (w/v) with optimum at 0%-7% (w/v). Phylogenetic analyses
A W, Duckworth   +5 more
openaire   +2 more sources

Transposon mutagenesis in Halomonas eurihalina.

Research in microbiology, 2000
We have established a transposon mutagenesis procedure for the moderate halophile Halomonas eurihalina, a bacteria that produces an exopolysaccharide (EPS) of considerable biotechnological interest. We used suicide plasmids pUT and pSUP102 to introduce the transposons mini-Tn5 and Tn1732 into H.
I, Llamas   +3 more
openaire   +1 more source

Novel insights into the role of potassium for osmoregulation in Halomonas elongata

Extremophiles, 2002
The role of K(+) in osmoregulation of the halophilic bacterium Halomonas elongata was investigated. At lower salinities (0.51 M NaCl), K(+) was the predominant cytoplasmic solute (1.25 micro mol mg protein(-1)). At higher salinities (1.03 M NaCl) ectoine became the main cytoplasmic solute (1.57 micro mol mg protein(-1)), while the K(+) content remained
Annette, Kraegeloh, Hans Jörg, Kunte
openaire   +2 more sources

Controlling cell volume for efficient PHB production by Halomonas

Metabolic Engineering, 2017
Bacterial morphology is decided by cytoskeleton protein MreB and cell division protein FtsZ encoded by essential genes mreB and ftsZ, respectively. Inactivating mreB and ftsZ lead to increasing cell sizes and cell lengths, respectively, yet seriously reduce cell growth ability.
Xiao-Ran, Jiang   +2 more
openaire   +2 more sources

Halomonas pelophila sp. nov., isolated from mud and Halomonas aquatica sp. nov., isolated from marine water

Antonie van Leeuwenhoek
Two Gram-negative, catalase- and oxidase-positive, cream-coloured, short rod-shaped, and motile bacterial strains, designated CS7T and SSM6T, were isolated from mud and marine water collected in Incheon, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belonged to the genus Halomonas.
Subin, Yook   +5 more
openaire   +2 more sources

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