Results 1 to 10 of about 2,643 (127)

Osmolality-Independent Impact of Sodium on Glycosylation of an Fc-Fusion Protein and the Hexosamine Biosynthesis Pathway in a Chinese Hamster Ovary Cell Line. [PDF]

Biotechnol J
Graphical Abstract and Lay Summary Sodium and potassium supplementation in CHO batch culture elevates intracellular UDP‐HexNAc concentrations through activation of the hexosamine biosynthesis pathway (HBP), independently of osmolality. Increased nucleotide sugar availability enhances Fc‐fusion protein glycosylation and reduces the abundance of low ...
Reiniger J, Pöttner A, Rosenberger L, Zimmer A.   +3 more
europepmc   +2 more sources

Exploring Ectoine Production From Methanol, Formate, and Electrochemically Produced Formate by <i>Methyloligella halotolerans</i>. [PDF]

Eng Life Sci
ABSTRACT Microbial synthesis using renewable C1‐carbon sources like electrochemically produced formate (e‐formate) represents a promising approach for climate‐neutral chemical production. This study investigates formate utilization for ectoine biosynthesis by the halophilic methylotroph Methyloligella halotolerans.
Kas A, Izadi P, Lenz C, Rohwerder T, Krömer JO, Harnisch F.   +5 more
europepmc   +2 more sources

An Efficient CO₂-Upcycling Platform Based on Engineered Halomonas TD with Enhanced Acetate-Utilizing Capacity via Adaptive Laboratory Evolution. [PDF]

Adv Sci (Weinh)
An electrochemical system is designed to convert CO2 into CO2‐derived electrolytes (CDE), mainly containing acetate and formate. The underlying mechanism of acetate metabolism in Halomonas TD80 is explored via ALE. Engineered TD80 produced high‐yield diversified products from CDE.
Wang C, Chen TT, Yang YJ, Li YX, Chang YX, Xiao YC, Guo WT, Zheng Y, Deng RZ, Tian YX, Situ W, Shen HW, Chen Y, Wang YB, Xing J, Wang H, Xia L, Lin YN, Ye JW.   +18 more
europepmc   +2 more sources

The biosynthesis of ectoine [PDF]

FEMS Microbiology Letters, 1990
The biosynthetic pathway of the novel compatible solute ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) was studied in the two extremely halophilic eubacteria Ectothiorhodospira halochloris and Halomonas elongata. The pathway starts with the phosphorylation of l-aspartate and shares its first two enzymatic steps with the biosynthesis
Petra Peters, E.A. Galinski, H.G. Trüper   +2 more
openaire   +1 more source

Ectoine Biosynthesis in Mycobacterium smegmatis [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACT Mycobacterium smegmatis is a commonly used mycobacterial model system. Here, we show that M. smegmatis protects itself against elevated salinity by synthesizing ectoine and hydroxyectoine and characterize the phenotype of a nonproducing mutant. This is the first analysis of
N. Ofer, M. Wishkautzan, M. Meijler, Y. Wang, A. Speer, M. Niederweis, E. Gur   +6 more
openaire   +2 more sources

Ectoine-Induced Proteins in Sinorhizobium meliloti Include an Ectoine ABC-Type Transporter Involved in Osmoprotection and Ectoine Catabolism [PDF]

Journal of Bacteriology, 2005
ABSTRACT To understand the mechanisms of ectoine-induced osmoprotection in Sinorhizobium meliloti , a proteomic examination of S. meliloti cells grown in minimal medium supplemented with ectoine was undertaken. This revealed the induction of 10 proteins.
Jebbar, Mohamed, Sohn-Bösser, Linda, Bremer, Erhard, Bernard, Théophile, Blanco, Carlos   +4 more
openaire   +2 more sources

Isolation of an ectoine-producing Sinobaca sp. and identification of genes that are involved in ectoine biosynthesis

FEMS Microbiology Letters, 2022
Abstract A moderate halophilic bacterium that could accumulate ectoine and hydroxyectoine was isolated from soil near a salt mine and was identified as a Sinobaca sp. (designed strain H24) according to 16S rRNA gene sequence analysis. The bacterium grew well in the presence of 1–2 M NaCl, while growth in a medium that contained 2 M NaCl ...
Shan-Yu Chen, Tzu-Chia Peng, Shan-Ze Huang, Chih-Ching Chien   +3 more
openaire   +2 more sources

Ultra‐Low‐Cost Hydrophobic Organic Coating for Highly Reversible Zinc Anodes

Angewandte Chemie, Volume 138, Issue 11, 9 March 2026.
A nanoscale hydrophobic 1,3‐Di(o‐tolyl)thiourea (DTH) layer (<14 nm) was deposited on zinc anodes for durable aqueous Zn‐ion batteries, costing merely 1.43×10−7 USD·Ah−1. By suppressing side reactions and dendrites, it achieved 2500‐cycle coin cells and a 143 Wh·kg−1 pouch cell (1200 cycles) with a record‐low additive cost (7.02×10−7 USD).
Shixun Wang, Zhiquan Wei, Yiqiao Wang, Shengnan Wang, Dedi Li, Hu Hong, Chuan Li, Yanbo Wang, Zhuoxi Wu, Shaoce Zhang, Xueying Zheng, Yi‐Chun Lu, Chunyi Zhi   +12 more
wiley   +2 more sources

Metagenomic Association Uncovers Host Genotype‐Structured Rhizobacterial Networks and Novel Taxa That Enhance Soybean Salt Tolerance

Advanced Science, EarlyView.
An STI‐guided metagenomic association framework links soybean salt‐tolerance phenotypes with rhizosphere microbiome composition and network organization in natural saline soils. The approach identifies candidate beneficial taxa, including Thalassospira xiamenensis, and provides an efficient strategy for mining stress‐protective microbes from native ...
Yu Luo, Feng‐Li Kang, Qi‐Min Li, Wei‐Cai Yang   +3 more
wiley   +1 more source

Distinct Microbiota and Functional Pathway Profiles Define Success and Failure in Regenerative Endodontic Treatment

International Endodontic Journal, EarlyView.
ABSTRACT Aim To characterize the intracanal microbiota and identify key microbial taxa and functional pathways associated with regenerative endodontic treatment (RET) outcomes. Methodology In this observational cohort study, 196 samples were collected at five time points from external tooth surfaces (T1, T4) and root canals (T2, T3, T5).
Leimarembi Devi Naorem, Alina Wikström, Peter Quach, Lokeshwaran Manoharan, Ronald Ordinola‐Zapata, Olena Rakhimova, Malin Brundin, Nelly Romani Vestman   +7 more
wiley   +1 more source