Development of an immunoFET biosensor for the detection of biotinylated PCR product
Heliyon, 2016 ImmunoFET (IMFET) biosensor is a simple platform for the detection of biotinylated products of polymerase chain reaction (PCR). Construction of the IMFET biosensor started with adsorption of 1.5 mg/mL of protein A (PA) onto the insulated gate surface of ...
Wannaporn Muangsuwan +8 more
doaj +1 more source
Virus‐Host and CRISPR Dynamics in Archaea‐Dominated Hypersaline Lake Tyrrell, Victoria, Australia
Archaea, Volume 2013, Issue 1, 2013., 2013 The study of natural archaeal assemblages requires community context, namely, a concurrent assessment of the dynamics of archaeal, bacterial, and viral populations. Here, we use filter size‐resolved metagenomic analyses to report the dynamics of 101 archaeal and bacterial OTUs and 140 viral populations across 17 samples collected over different ...
Joanne B. Emerson +7 more
wiley +1 more source
A comparative genomics perspective on the genetic content of the alkaliphilic haloarchaeon Natrialba magadii ATCC 43099T [PDF]
, 2012 BACKGROUND: Natrialba magadii is an aerobic chemoorganotrophic member of the Euryarchaeota and is a dual extremophile requiring alkaline conditions and hypersalinity for optimal growth. The genome sequence of Nab.
Diego E Sastre +15 more
core +2 more sources
PH1: An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV‐2
Archaea, Volume 2013, Issue 1, 2013., 2013 Halovirus PH1 infects Haloarcula hispanica and was isolated from an Australian salt lake. The burst size in single‐step growth conditions was 50–100 PFU/cell, but cell density did not decrease until well after the rise (4–6 hr p.i.), indicating that the virus could exit without cell lysis.
Kate Porter +6 more
wiley +1 more source
Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: Investigating acetamidase gene function [PDF]
, 2016 © 2016 The Author(s). No systems have been reported for genetic manipulation of cold-adapted Archaea. Halorubrum lacusprofundi is an important member of Deep Lake, Antarctica (∼10% of the population), and is amendable to laboratory cultivation.
Cavicchioli, R +7 more
core +1 more source
Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes [PDF]
, 2007 Background The presence of the branched Entner-Doudoroff (ED) pathway in two hyperthermophilic Crenarchaea, the anaerobe Thermoproteus tenax and the aerobe Sulfolobus solfataricus, was suggested.
Daniel Kehrer +3 more
core +2 more sources
SICLE: A high-throughput tool for extracting evolutionary relationships from phylogenetic trees
, 2016 We present the phylogeny analysis software SICLE (Sister Clade Extractor), an easy-to-use, high- throughput tool to describe the nearest neighbors to a node of interest in a phylogenetic tree as well as the support value for the relationship.
DeBlasio, Dan, Wiscaver, Jennifer
core +2 more sources
Functional analysis of archaeal MBF1 by complementation studies in yeast [PDF]
, 2011 BACKGROUND: Multiprotein-bridging factor 1 (MBF1) is a transcriptional co-activator that bridges a sequence-specific activator (basic-leucine zipper (bZIP) like proteins (e.g. Gcn4 in yeast) or steroid/nuclear-hormone receptor family (e.g.
Jeannette Marrero Coto +3 more
core +3 more sources
The biology of habitat dominance; can microbes behave as weeds? [PDF]
, 2013 Competition between microbial species is a product of, yet can lead to a reduction in, the microbial diversity of specific habitats. Microbial habitats can resemble ecological battlefields where microbial cells struggle to dominate and/or annihilate each
Bell, Andrew N. W. +5 more
core +1 more source
Comparative functional genomics approach for the annotation of proteins in Unclassified Halophilic archaeon DL31 [PDF]
, 2012 The structure, function and sub-cellular location prediction for the unknown proteins from Unclassified Halophilic archaeon DL31 were carried out for characterization of the proteins in their respective families.
E Nakkeeran +2 more
core +1 more source