Single Mutation in Hammerhead Ribozyme Favors Cleavage Activity with Manganese over Magnesium [PDF]
Non-Coding RNA, 2020 Hammerhead ribozymes are one of the most studied classes of ribozymes so far, from both the structural and biochemical point of views. The activity of most hammerhead ribozymes is cation-dependent. Mg2+ is one of the most abundant divalent cations in the
Mohammad Reza Naghdi +4 more
doaj +5 more sources
The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme [PDF]
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
doaj +3 more sources
The Hammerhead Ribozyme: A Long History for a Short RNA [PDF]
Molecules, 2017 Small nucleolytic ribozymes are a family of naturally occurring RNA motifs that catalyse a self-transesterification reaction in a highly sequence-specific manner.
Marcos de la Peña +2 more
doaj +3 more sources
A High-Pressure, High-Temperature Flow Reactor Simulating the Hadean Earth Environment, with Application to the Pressure Dependence of the Cleavage of Avocado Viroid Hammerhead Ribozyme [PDF]
Life, 2022 The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system.
Kunio Kawamura +8 more
doaj +4 more sources
Life on Minerals: Binding Behaviors of Oligonucleotides on Zirconium Silicate and Its Inhibitory Activity for the Self-Cleavage of Hammerhead Ribozyme [PDF]
Life, 2022 The role of minerals in the chemical evolution of RNA molecules is an important issue when considering the early stage of the Hadean Earth. In particular, the interaction between functional ribozymes and ancient minerals under simulated primitive ...
Kunio Kawamura +5 more
doaj +4 more sources
The ubiquitous hammerhead ribozyme. [PDF]
RNA, 2012 The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self-scission under physiological conditions. Originally discovered in subviral plant pathogens, its presence
Hammann C +3 more
europepmc +7 more sources
Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds [PDF]
FEBS Open Bio, 2014 Nucleic acids are useful for biomedical targeting and sensing applications in which the molecular environment is different from that of a dilute aqueous solution. In this study, the influence of various types of mixed solutions of water and water-soluble
Shu-ichi Nakano +3 more
doaj +3 more sources
Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme. [PDF]
Genome Biol, 2023 Background CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness.
Zhan Y, Cao C, Li A, Mei H, Liu Y.
europepmc +2 more sources
The hammerhead ribozyme: structure, catalysis, and gene regulation. [PDF]
Prog Mol Biol Transl Sci, 2013 Scott WG, Horan LH, Martick M.
europepmc +4 more sources
Molecular simulations of the pistol ribozyme: unifying the interpretation of experimental data and establishing functional links with the hammerhead ribozyme. [PDF]
RNA, 2019 The pistol ribozyme (Psr) is among the most recently discovered RNA enzymes and has been the subject of experiments aimed at elucidating the mechanism.
Kostenbader K, York DM.
europepmc +2 more sources