A High-Pressure, High-Temperature Flow Reactor Simulating the Hadean Earth Environment, with Application to the Pressure Dependence of the Cleavage of Avocado Viroid Hammerhead Ribozyme [PDF]
Life, 2022 The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system.
Kunio Kawamura +8 more
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Life on Minerals: Binding Behaviors of Oligonucleotides on Zirconium Silicate and Its Inhibitory Activity for the Self-Cleavage of Hammerhead Ribozyme [PDF]
Life, 2022 The role of minerals in the chemical evolution of RNA molecules is an important issue when considering the early stage of the Hadean Earth. In particular, the interaction between functional ribozymes and ancient minerals under simulated primitive ...
Kunio Kawamura +5 more
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The Hammerhead Ribozyme: A Long History for a Short RNA [PDF]
Molecules, 2017 Small nucleolytic ribozymes are a family of naturally occurring RNA motifs that catalyse a self-transesterification reaction in a highly sequence-specific manner.
Marcos de la Peña +2 more
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The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme [PDF]
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
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A hammerhead ribozyme selects mechanically stable conformations for catalysis against viral RNA [PDF]
Communications BiologyRibozymes, widely found in prokaryotes and eukaryotes, target nucleic acids and can be engineered as biotechnical tools or for gene regulation or immune therapy. Among them, hammerhead is the smallest and best characterized ribozyme.
Man Lu +11 more
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Single Mutation in Hammerhead Ribozyme Favors Cleavage Activity with Manganese over Magnesium [PDF]
Non-Coding RNA, 2020 Hammerhead ribozymes are one of the most studied classes of ribozymes so far, from both the structural and biochemical point of views. The activity of most hammerhead ribozymes is cation-dependent. Mg2+ is one of the most abundant divalent cations in the
Mohammad Reza Naghdi +4 more
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Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst? [PDF]
ChembiochemRational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Ebermann C, Müller S.
europepmc +2 more sources
Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.). [PDF]
Plant JSUMMARY Genome editing using the CRISPR/Cas system enables rapid and efficient plant breeding by directly introducing desired traits into elite lines within a short time frame. However, challenges associated with conventional Agrobacterium tumefaciens‐mediated transformation and regeneration have limited gene editing in pepper (Capsicum annuum L.).
Kang B +6 more
europepmc +2 more sources
Effects of lipid membranes on RNA catalytic activity and stability. [PDF]
Biol CellThis study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Czerniak T, Saenz JP.
europepmc +2 more sources
Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds [PDF]
FEBS Open Bio, 2014 Nucleic acids are useful for biomedical targeting and sensing applications in which the molecular environment is different from that of a dilute aqueous solution. In this study, the influence of various types of mixed solutions of water and water-soluble
Shu-ichi Nakano +3 more
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