Results 91 to 100 of about 19,908 (281)

Classification of the nucleolytic ribozymes based upon catalytic mechanism [PDF]

, 2019
The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2’-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater.
A Ferré-D'Amaré, A Forster, A Hampel, A Hampel, A Ke, A McLeod, A Perrotta, A Perrotta, A Ren, A Ren, A Roth, B Saville, B Saville, C Gaines, C Gaines, C Gaines, C Hutchins, D Eiler, D Klein, D Klein, F Jones, F Westheimer, G Slim, G Soukup, H van Tol, J Buzayan, J Cochrane, J Han, J Haseloff, J Murray, J Thomas, K Harris, K Young, L Hegg, L Kapinos, L Nguyen, L Zheng, M Canny, M Kuo, M Martick, M Oivanen, N Suslov, P Bevilacqua, P Rupert, R Breaker, S Das, S Kath-Schorr, S Koo, S Li, S Nakano, T Lee, T Wilson, T Wilson, T Wilson, T Wilson, W Winkler, Y Komatsu, Y Li, Y Liu, Y Liu, Z Weinberg, Z Weinberg   +61 more
core   +3 more sources

Folding of the hammerhead ribozyme: pyrrolo-cytosine fluorescence separates core folding from global folding and reveals a pH-dependent conformational change.

RNA: A publication of the RNA Society, 2012
The catalytic activity of the hammerhead ribozyme is limited by its ability to fold into the native tertiary structure. Analysis of folding has been hampered by a lack of assays that can independently monitor the environment of nucleobases throughout the
Iwona A. Buskiewicz, John M. Burke
semanticscholar   +1 more source

Ribozyme-based insulator parts buffer synthetic circuits from genetic context [PDF]

, 2012
Synthetic genetic programs are built from circuits that integrate sensors and implement temporal control of gene expression. Transcriptional circuits are layered by using promoters to carry the signal between circuits. In other words, the output promoter
A Arkin, A Arkin, A Khvorova, AC Forster, AL Delcher, AM Walczak, B Munsky, B Munsky, B Munsky, B Wang, Brian Munsky, Brynne Stanton, C Hernández, C Lou, C Tan, CA Voigt, Christopher A Voigt, Chunbo Lou, D Del Vecchio, D Endy, D-K Ro, DT Gillespie, F Di Serio, HM Salis, J Beal, JA Nelson, JC Anderson, JC Anderson, JH Davis, JM Buzayan, JM Kaper, JM Pedraza, JR Kelly, K Clancy, K Temme, M Galdzicki, MB Elowitz, MJ Roossinck, PM Kim, R Grünberg, R Lutz, RP Shetty, S Hooshangi, T Ellis, TA Carrier, TS Gardner, VA Rhodius, Y Yokobayashi, Ying-Ja Chen   +48 more
core   +1 more source

Targeting Triple‐Negative Breast Cancer: Resistance Mechanisms and Therapeutic Advancements

Cancer Medicine, Volume 14, Issue 9, May 2025.
ABSTRACT Background Triple‐negative breast cancer (TNBC) is one of the most heterogeneous and menacing forms of breast cancer, with no sustainable cure available in the current treatment landscape. Its lack of targets makes it highly unresponsive to various treatment modalities, which is why chemotherapy continues to be the primary form of treatment ...
Rachana Raman, Shristi Debata, Thirupugal Govindarajan, Praveen Kumar   +3 more
wiley   +1 more source

RNA Back and Forth: Looking through Ribozyme and Viroid Motifs

Viruses, 2019
Current cellular facts allow us to follow the link from chemical to biochemical metabolites, from the ancient to the modern world. In this context, the “RNA world„ hypothesis proposes that early in the evolution of life, the ribozyme was ...
Marie-Christine Maurel, Fabrice Leclerc, Jacques Vergne, Giuseppe Zaccai   +3 more
doaj   +1 more source

Enterovirus A71 DNA-Launched Infectious Clone as a Robust Reverse Genetic Tool. [PDF]

PLoS ONE, 2016
Enterovirus A71 (EV-A71) causes major outbreaks of hand, foot and mouth disease, and is occasionally associated with neurological complications and death in children.
Chee Wah Tan, Han Kang Tee, Michelle Hui Pheng Lee, I-Ching Sam, Yoke Fun Chan   +4 more
doaj   +1 more source

Nucleozymes [PDF]

, 2004
Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts.
Cedergren, Robert J., Perreault, Jean-Pierre, Rich, Alexander, Usman, Nassim, Yang, Jing-Hua   +4 more
core   +1 more source

Force for ancient and recent life: viral and stem-loop RNA consortia promote life. [PDF]

, 2014
Lytic viruses were thought to kill the most numerous host (i.e., kill the winner). But persisting viruses/defectives can also protect against viruses, especially in a ubiquitous virosphere. In 1991, Yarmolinsky et al.
Villarreal, Luis P
core   +1 more source

Simple and Efficient Transformation and Gene Editing of Marchantia polymorpha Spores

Current Protocols, Volume 5, Issue 5, May 2025.
Abstract Marchantia polymorpha (Marchantia) has become a model species for liverwort studies, owing to its rapid growth in vitro, ease of propagation, simple genetics, small genome, haploid‐dominant life cycle, and because it is amenable to genetic transformation.
Rebecca M. Yorker, Simon C. Deroles, Yanfei Zhou, Jennifer A. Tate, Kevin M. Davies, Nick W. Albert   +5 more
wiley   +1 more source

Hammerhead Ribozymes: True Metal or Nucleobase Catalysis? Where Is the Catalytic Power from?

Molecules, 2010
The hammerhead ribozyme was first considered as a metalloenzyme despite persistent inconsistencies between structural and functional data. In the last decade, metal ions were confirmed as catalysts in self-splicing ribozymes but displaced by nucleobases ...
Fabrice Leclerc
doaj   +1 more source