Results 91 to 100 of about 16,494 (236)

A modern mode of activation for nucleic acid enzymes.

PLoS ONE, 2007
Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficiently high substrate specificity required by modern cellular metabolism. One of these modes is the use of a target-dependent module (i.e. a docking domain)
Dominique Lévesque, Francis P Brière, Jean-Pierre Perreault   +2 more
doaj   +1 more source

Computational De Novo Design of Group II Introns Yields Highly Active Ribozymes

ChemBioChem, Volume 26, Issue 14, July 18, 2025.
Group II Introns (G2Is) are large self‐splicing ribozymes with promising biotechnological applications. This study utilized RNA inverse folding to design three novel G2Is. The designed intron Arq.I2, an unexpectedly proficient ribozyme, self‐splices not only in vitro, but also in Escherichia coli cells.
Deni Szokoli, Noemi E. Nwosu, Lukas M. Glatt, Hannes Mutschler   +3 more
wiley   +1 more source

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.

PLoS ONE, 2020
Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen, Mika Schievelbein, Kushkumar Patel, Davis Jose, Jonathan Ouellet   +4 more
doaj   +1 more source

Ribozyme-mediated gene knock down strategy to dissect the consequences of PDGF stimulation in vascular smooth muscle cells

BMC Research Notes, 2012
Background Vascular Smooth Muscle Cells (VSMCs), due to their plasticity and ability to shift from a physiological contractile-quiescent phenotype to a pathological proliferating-activated status, play a central role in the onset and progression of ...
Lande Caterina, Boccardi Claudia, Citti Lorenzo, Mercatanti Alberto, Rizzo Milena, Rocchiccioli Silvia, Tedeschi Lorena, Trivella Maria, Cecchettini Antonella   +8 more
doaj   +1 more source

Ribozyme-Mediated Knockdown of lncRNA Gene Expression in Drosophila

Bio-Protocol
Long noncoding RNAs (lncRNAs) are increasingly understood to play important roles in cell biology, development, and disease, though the vast majority of annotated lncRNAs have yet to be functionally characterized.
Kevin Nyberg, Richard Carthew
doaj   +1 more source

Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs

Frontiers in Microbiology, 2012
As a consequence of two unique physical properties, small size and circularity, viroid RNAs do not code for proteins and thus depend on RNA sequence/structural motifs for interacting with host proteins that mediate their invasion, replication, spread ...
Ricardo eFlores, Pedro eSerra, Sofia eMinoia, Francesco eDi Serio, Beatriz eNavarro   +4 more
doaj   +1 more source

Hammerhead ribozyme-based U-insertion and deletion RNA editing assays for multiplexing in HTS applications. [PDF]

RNA, 2023
Rostamighadi M, Mehta V, Hassan Khan R, Moses D, Salavati R.   +4 more
europepmc   +1 more source

An adaptive defect weighted sampling algorithm to design pseudoknotted RNA secondary structures

Frontiers in Genetics, 2016
Computational design of RNA sequences that fold into targeted secondary structures hasmany applications in biomedicine, nanotechnology and synthetic biology.
Kasra Zandi, Gregory Butler, Nawwaf Kharma, Nawwaf Kharma   +3 more
doaj   +1 more source

Preparations of hammerhead ribozymes for investigations of their cleavable sequences [PDF]

, 2009
H. Tateoka, Ichiro Kawahara, Shingo Hasegawa, K. Haruta, Yoshinori Kondo, Chojiro Kojima, Yutaro Tanaka   +6 more
openalex   +1 more source

Avsunviroidae family: Viroids containing hammerhead ribozymes

, 2000
This chapter focuses on the second viroid family, whose members are also referred to as hammerhead viroids, taking into account their most outstanding feature. If the word “small” is the first to come to mind when considering viroids, perhaps the second word is “hammerhead,” because this class of ribozymes, which because of its structural simplicity ...
Flores, Ricardo, Daròs, Jose-Antonio, Hernández, Carmen   +2 more
openaire   +2 more sources