Results 111 to 120 of about 19,908 (281)
A ligand-dependent hammerhead ribozyme switch for controlling mammalian gene expression.
Molecular Biosystems, 2010 The possibility to externally control gene expression is of fundamental importance in both basic and applied life sciences. Although there are some techniques available to regulate expression in mammalian cells, they rely on the presence of ligand ...
Simon Ausländer +2 more
semanticscholar +1 more source
, 2009 Ribozimi su katalitičke molekule koje moraju biti savijene u visoko uređene tercijarne strukture, kao što to proteini rade da bi obavljali svoje funkcije. Glavni cilj je dovesti nukleofil i izrezujući fosfat u blizinu i aktivirati ih.
Zoko, Nikolina
core +2 more sources
Biophysical and biochemical investigations of RNA catalysis in the hammerhead ribozyme [PDF]
, 1999 1. How do ribozymes work? 241 2. The hammerhead RNA as a prototype ribozyme 242 2.1 RNA enzymes 242 2.2 Satellite self-cleaving RNAs 242 2.3 Hammerhead RNAs and hammerhead ribozymes 244 3.
Scott, William G
core +2 more sources
Computational mutagenesis studies of hammerhead ribozyme catalysis.
Journal of the American Chemical Society, 2010 Computational studies of the mutational effects at the C3, G8, and G5 positions of the hammerhead ribozyme (HHR) are reported, based on a series of twenty-four 100-ns molecular dynamics simulations of the native and mutated HHR in the reactant state and ...
Tai-Sung Lee, D. York
semanticscholar +1 more source
The prebiotic evolutionary advantage of transferring genetic information from RNA to DNA. [PDF]
, 2011 In the early 'RNA world' stage of life, RNA stored genetic information and catalyzed chemical reactions. However, the RNA world eventually gave rise to the DNA-RNA-protein world, and this transition included the 'genetic takeover' of information storage ...
Chen, Irene A +4 more
core +4 more sources
Solvent Structure and Hammerhead Ribozyme Catalysis
Chemistry & Biology, 2008 Although the hammerhead ribozyme is regarded as a prototype for understanding RNA catalysis, the mechanistic roles of associated metal ions and water molecules in the cleavage reaction remain controversial. We have investigated the catalytic potential of observed divalent metal ions and water molecules bound to a 2 A structure of the full-length ...
William G. Scott +4 more
openaire +4 more sources
A modern mode of activation for nucleic acid enzymes.
PLoS ONE, 2007 Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficiently high substrate specificity required by modern cellular metabolism. One of these modes is the use of a target-dependent module (i.e. a docking domain)
Dominique Lévesque +2 more
doaj +1 more source
A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.
PLoS ONE, 2020 Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen +4 more
doaj +1 more source
The Plant Journal, Volume 121, Issue 3, February 2025.Significance Statement We present an example of an editing factor with a synthetic P‐type PPR tract capable of precise, targeted de novo RNA editing in plant mitochondria. This breakthrough enables sophisticated manipulation of gene expression in mitochondria, which have long been genetically inaccessible, and holds promise for applications not only in
Nikolay Manavski +6 more
wiley +1 more source
Intracellular Selection of Theophylline-Sensitive Hammerhead Aptazyme
Molecular Therapy: Nucleic Acids, 2020 Hammerhead ribozyme-based aptazyme (HHAz), inheriting the advantages of small size and high efficiency from the RNA-cleaving ribozyme and the specific recognition ability of aptamers to specific targets, exhibits the huge potential to be a transgene ...
Qinlin Pu +8 more
doaj