Results 121 to 130 of about 19,908 (281)

Ribozyme-mediated gene knock down strategy to dissect the consequences of PDGF stimulation in vascular smooth muscle cells

BMC Research Notes, 2012
Background Vascular Smooth Muscle Cells (VSMCs), due to their plasticity and ability to shift from a physiological contractile-quiescent phenotype to a pathological proliferating-activated status, play a central role in the onset and progression of ...
Lande Caterina, Boccardi Claudia, Citti Lorenzo, Mercatanti Alberto, Rizzo Milena, Rocchiccioli Silvia, Tedeschi Lorena, Trivella Maria, Cecchettini Antonella   +8 more
doaj   +1 more source

Biochemical Characterization of a Lanthanide-Dependent DNAzyme with Normal and Phosphorothioate-Modified Substrates [PDF]

, 2015
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biochemistry, copyright © American Chemical Society after peer review and technical editing by publisher.
Huang, Po-Jung Jimmy, Liu, Juewen, Matuszek, Zaneta, Vazin, Mahsa   +3 more
core   +1 more source

Metal ion specificities for folding and cleavage activity in the Schistosoma hammerhead ribozyme.

RNA: A publication of the RNA Society, 2008
The effects of various metal ions on cleavage activity and global folding have been studied in the extended Schistosoma hammerhead ribozyme. Fluorescence resonance energy transfer was used to probe global folding as a function of various monovalent and ...
J. L. Boots, M. Canny, E. Azimi, A. Pardi   +3 more
semanticscholar   +1 more source

Robust and stable transcriptional repression in Giardia using CRISPRi. [PDF]

, 2019
Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the ...
Booker, J, Dawson, Scott C, Hagen, KD, Jones, K, McInally, SG, Nguyen, K, Nosala, C, Williams, J   +7 more
core   +1 more source

PAM‐relaxed and temperature‐tolerant CRISPR‐Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato

Plant Biotechnology Journal, Volume 23, Issue 1, Page 156-173, January 2025.
Summary Class 2 Type V‐A CRISPR‐Cas (Cas12a) nucleases are powerful genome editing tools, particularly effective in A/T‐rich genomic regions, complementing the widely used CRISPR‐Cas9 in plants. To enhance the utility of Cas12a, we investigate three Cas12a orthologs—Mb3Cas12a, PrCas12a, and HkCas12a—in plants.
Shishi Liu, Yao He, Tingting Fan, Meirui Zhu, Caiyan Qi, Yanqin Ma, Mengqiao Yang, Liang Yang, Xu Tang, Jianping Zhou, Zhaohui Zhong, Xueli An, Yiping Qi, Yong Zhang   +13 more
wiley   +1 more source

Novel Fig-Associated Viroid-Like RNAs Containing Hammerhead Ribozymes in Both Polarity Strands Identified by High-Throughput Sequencing

Frontiers in Microbiology, 2020
Based on high-throughput sequencing (HTS) data, the existence of viroid-like RNAs (Vd-LRNAs) associated with fig trees grown in the Hawaiian Islands has been predicted.
Alejandro Olmedo-Velarde, Beatriz Navarro, John S. Hu, Michael J. Melzer, Francesco Di Serio   +4 more
doaj   +1 more source

Leakage and slow allostery limit performance of single drug-sensing aptazyme molecules based on the hammerhead ribozyme.

RNA: A publication of the RNA Society, 2008
Engineered "aptazymes" fuse in vitro selected aptamers with ribozymes to create allosteric enzymes as biosensing components and artificial gene regulatory switches through ligand-induced conformational rearrangement and activation.
Chamaree de Silva, N. Walter
semanticscholar   +1 more source

Elucidating Evolutionary Mechanisms and Variants of the Hammerhead Ribozyme Using In Vitro Selection

ChemBioChem, Volume 25, Issue 21, November 4, 2024.
We generated single‐, double‐, and triple‐mutation libraries to explore the sequence requirements and evolution of a full‐length hammerhead ribozyme. We identified many hammerhead variants, some of which possess mutations at previously considered essential nucleotides. We also present a novel methodological approach for evaluating the sequence‐function
Jake Brill, Connor Nurmi, Yingfu Li
wiley   +1 more source

Potent intracellular knock down of hepatitis B virus X RNA by catalytic hammerhead ribozymes or DNA-Enzymes with antisense DNA-oligonucleotides or 10-23 DNA-Enzymes that powerfully augment in vitro sequence-specific cleavage activities [PDF]

, 2008
Novel antiviral approaches are needed to control Hepatitis B virus infection worldwide. X protein of this virus activates various promoters and is strongly associated with hepatocellular carcinoma.
Banerjea, Akhil, Bano, Aalia, Gupta, Nidhi, Sharma, Yogeshwar, Sood, Vikas   +4 more
core   +1 more source

Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs

Frontiers in Microbiology, 2012
As a consequence of two unique physical properties, small size and circularity, viroid RNAs do not code for proteins and thus depend on RNA sequence/structural motifs for interacting with host proteins that mediate their invasion, replication, spread ...
Ricardo eFlores, Pedro eSerra, Sofia eMinoia, Francesco eDi Serio, Beatriz eNavarro   +4 more
doaj   +1 more source