A versatile cis-blocking and trans-activation strategy for ribozyme characterization [PDF]
, 2013 Synthetic RNA control devices that use ribozymes as gene-regulatory components have been applied to controlling cellular behaviors in response to environmental signals.
Kennedy, Andrew B. +2 more
core +1 more source
Direct Observation of Multiple Tautomers of Oxythiamine and their Recognition by the Thiamine Pyrophosphate Riboswitch [PDF]
, 2013 Structural diversification of canonical nucleic acid bases and nucleotide analogues by tautomerism has been proposed to be a powerful on/off switching mechanism allowing regulation of many biological processes mediated by RNA enzymes and aptamers ...
Essigmann, John M. +6 more
core +1 more source
An adaptive defect weighted sampling algorithm to design pseudoknotted RNA secondary structures
Frontiers in Genetics, 2016 Computational design of RNA sequences that fold into targeted secondary structures hasmany applications in biomedicine, nanotechnology and synthetic biology.
Kasra Zandi +3 more
doaj +1 more source
Development of a Chimeric DNA-RNA Hammerhead Ribozyme Targeting SARS Virus
Intervirology, 2009 Objective: Severe acute respiratory syndrome (SARS) is a severe pulmonary infectious disease caused by a novel coronavirus. To develop an effective and specific medicine targeting the SARS-coronavirus (CoV), a chimeric DNA-RNA hammerhead ribozyme was ...
A. Fukushima +8 more
semanticscholar +1 more source
Role of Mg2+ in hammerhead ribozyme catalysis from molecular simulation.
Journal of the American Chemical Society, 2008 Molecular dynamics simulations have been performed to investigate the role of Mg2+ in the full-length hammerhead ribozyme cleavage reaction. In particular, the aim of this work is to characterize the binding mode and conformational events that give rise ...
Tai-Sung Lee +5 more
semanticscholar +1 more source
eLife, 2016 The nonenzymatic replication of RNA is a potential transitional stage between the prebiotic chemistry of nucleotide synthesis and the canonical RNA world in which RNA enzymes (ribozymes) catalyze replication of the RNA genomes of primordial cells ...
Noam Prywes +3 more
doaj +1 more source
Hammerhead ribozyme-based U-insertion and deletion RNA editing assays for multiplexing in HTS applications. [PDF]
RNA, 2023 Rostamighadi M +4 more
europepmc +1 more source
Processing of insect retrotransposons by self-cleaving ribozymes [PDF]
, 2010 We show that several classes of insect non-LTR retrotransposons harbor self-cleaving ribozymes of the HDV family at their 5′ termini. In Drosophila the R2 ribozymes exhibit highly differential in vivo expression and robust in vitro activity ...
Andrej Luptak +3 more
core +1 more source
Probing the hammerhead ribozyme structure with ribonucleases
Nucleic Acids Research, 1994 Susceptibility to RNase digestion has been used to probe the conformation of the hammerhead ribozyme structure prepared from chemically synthesised RNAs. Less than about 1.5% of the total sample was digested to obtain a profile of RNase digestion sites.
Neil J. Shirley +2 more
openaire +4 more sources
The model structure of the hammerhead ribozyme formed by RNAs of reciprocal chirality. [PDF]
Biosci Rep, 2021 Wyszko E +8 more
europepmc +1 more source