Results 131 to 140 of about 6,878 (169)

Photochemical hammerhead ribozyme activation

Bioorganic & Medicinal Chemistry Letters, 2006
We report the light-activation of allosteric cis and trans acting ribozymes via decaging of a small organic molecule ligand. To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function.
Douglas D. Young, Alexander Deiters
openaire   +3 more sources

Folding and Activity of the Hammerhead Ribozyme

ChemBioChem, 2002
AbstractFor Abstract see ChemInform Abstract in Full Text.
David M.J. Lilley, Christian Hammann
openaire   +4 more sources

Small, Efficient Hammerhead Ribozymes

Molecular Biotechnology, 2000
The hammerhead ribozyme is able to cleave RNA in a sequence-specific manner. These ribozymes are usually designed with four basepairs in helix II, and with equal numbers of nucleotides in the 5' and 3' hybridizing arms that bind the RNA substrate on either side of the cleavage site.
Maxine J. McCall, Philip Hendry, Trevor Lockett, Glenn S. Brown, Jason Conaty, Alain A. Mir   +5 more
openaire   +3 more sources

Hammerhead ribozyme design and application

Cellular and Molecular Life Sciences CMLS, 1998
The emerging knowledge about RNA-based enzymes has already had great impact on our concept of evolutionary history, making the 'RNA world' more likely. It may well have an equally important impact on the diagnostic and therapeutic practices of human and veterinary medicine in the next decade. We are not quite there yet. This review addresses the design
M Amarzguioui, H Prydz
openaire   +2 more sources

Characterization of Hammerhead Ribozyme Reactions

2012
Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes.
Carsten Seehafer, Christian Hammann, Anne Kalweit, Marcos de la Peña, Rita Przybilski   +4 more
openaire   +3 more sources

Thermodynamic Dissection of the Substrate−Ribozyme Interaction in the Hammerhead Ribozyme

Biochemistry, 1998
The free energy of substrate binding to the hammerhead ribozyme was compared for 10 different hammerheads that differed in the length and sequence of their substrate recognition helices. These hammerheads were selected because neither ribozyme nor substrate oligonucleotide formed detectable alternate secondary structures.
Tracy K. Stage-Zimmermann, Glenyss E. Ammons, Olke C. Uhlenbeck, Klemens J. Hertel   +3 more
openaire   +3 more sources

The internal equilibrium of the hammerhead ribozyme reaction

Biochemistry, 1995
The effects of temperature, pH, and magnesium ion concentration on the internal equilibrium of the hammerhead ribozyme reaction were determined in order to better understand why the ribozyme-bound substrate RNA is 99% cleaved at equilibrium. Cleavage of substrate is more efficient at higher temperatures because a large entropy gain upon cleavage ...
Olke C. Uhlenbeck, Klemens J. Hertel
openaire   +3 more sources

Hammerhead ribozymes for target validation

Expert Opinion on Therapeutic Targets, 2002
Expensive failures in the pharmaceutical industry might be avoided by target validation at an early stage. Often, the full consequences of inhibiting a chosen drug target do not emerge until late in the development process. One option is to use hammerhead ribozymes as highly specific ribonucleases targeted exclusively at the mRNA encoding the target ...
openaire   +4 more sources

RNA Folding and Misfolding of the Hammerhead Ribozyme

Biochemistry, 1999
The hammerhead ribozyme undergoes a well-defined two-stage folding process induced by the sequential binding of two magnesium ions. These probably correspond to the formation of domain 2 (0-500 microM magnesium ions) and domain 1 (1-20 mM magnesium ions), respectively.
Gurminder S. Bassi, Niels Erik Møllegaard, David M.J. Lilley, A. I. H. Murchie   +3 more
openaire   +3 more sources

Genomisch codierte Hammerhead-Ribozyme

BIOspektrum, 2012
The hammerhead ribozyme is a small RNA motif capable to cleave RNA endonucleolytically. Originally discovered in sub-viral plant pathogens, recent studies have uncovered the presence of this ribozyme in an unprecedented number of bacterial and eukaryal genomes.
Christian Hammann, Carsten Seehafer, Anne Kalweit   +2 more
openaire   +2 more sources