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A tale in molecular recognition: the hammerhead ribozyme
Journal of Molecular Recognition, 2006AbstractA new crystal structure of the hammerhead ribozyme demonstrates the influence of peripheral tertiary contacts on the local conformations around the active site. This structure resolves many conflicting results obtained on reduced systems. Copyright © 2006 John Wiley & Sons, Ltd.
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Protein Enhancement of Hammerhead Ribozyme Catalysis
Science, 1993When the recognition sequence of a ribozyme is extended beyond a certain length, turnover is slowed and specificity is decreased. Here, it is shown that a protein can help a ribozyme overcome these general limitations on ribozyme activity. Cleavage of an RNA oligonucleotide by a hammerhead ribozyme is enhanced 10- to 20-fold upon addition of a protein ...
Mala Khosla +2 more
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Degradation of hammerhead ribozymes by human ribonucleases
Molecular and General Genetics MGG, 1998Hammerhead ribozymes were used as substrates to examine endoribonucleolytic activities in cell extracts and cultured human cells. Primer-extension analyses showed that ribozymes directed against tumor necrosis factor-alpha mRNA and human immunodeficiency virus type 1 tat mRNA were cleaved at UA and CA dinucleotides by extracts. Preferred cleavage sites
G. Kaplan +6 more
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Structure—function studies of the hammerhead ribozyme
Current Opinion in Chemical Biology, 1997Elucidation of the catalytic mechanism and structure-function relationship studies of the hammerhead ribozyme continue to be an area of intensive research. A combination of diverse approaches, such as X ray crystallography, spectral studies, chemical modifications, sequence variations and kinetic analyses, have provided valuable insight into the ...
Fritz Eckstein +2 more
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Kinetics of intermolecular cleavage by hammerhead ribozymes
Biochemistry, 1992The hammerhead catalytic RNA effects cleavage of the phosphodiester backbone of RNA through a transesterification mechanism that generates products with 2'-3'-cyclic phosphate and 5'-hydroxyl termini. A minimal kinetic mechanism for the intermolecular hammerhead cleavage reaction includes substrate binding, cleavage, and product release. Elemental rate
Olke C. Uhlenbeck, Martha J. Fedor
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Application of hammerhead ribozymes in filamentous fungi
Journal of Microbiological Methods, 2006Metabolic engineering in filamentous fungi is a emerging field of research as many fungi produce high value primary and secondary metabolites. Ribozyme technology can be used as a tool for metabolic engineering to influence metabolic pathways and to knock down the expression of specific genes of interest.
Vera Meyer, Ulf Stahl, Dirk Mueller
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Hammerhead Ribozyme-Based Target Discovery
2006With the accumulation of vast amounts of data as a result of the sequencing of the human genome, it is necessary to identify human genes that are involved in various cellular, developmental, and disease-related processes and to clarify their functions and potential utility as targets in the treatment of disease.
Kazunari Taira, Masayuki Sano
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Cobalt Hexammine Inhibition of the Hammerhead Ribozyme
Biochemistry, 2000The effects of Co(NH(3))(6)(3+) on the hammerhead ribozyme are analyzed using several techniques, including activity measurements, electron paramagnetic resonance (EPR), and circular dichroism (CD) spectroscopies and thermal denaturation studies. Co(NH(3))(6)(3+) efficiently displaces Mn(2+) bound to the ribozyme with an apparent dissociation constant ...
Victoria J. DeRose, Thomas E. Horton
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Mechanistic Studies on Hammerhead Ribozymes
1996Catalytic RNAs include group I and II introns; the RNA subunit of RNase P; hammerhead, hairpin, and hepatitis delta virus ribozymes; and ribosomal RNA (Altman 1989; Cech 1989; Michel et al. 1989; Noller et al. 1992; Symons 1992; Bratty et al. 1993; Gesteland and Atkins 1993).
P. K. R. Kumar +3 more
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