The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme. [PDF]
Nat CommunWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozymethat closely resembles the pistol ribozyme, with essentially identical secondary structure andconnectivity.
Zhan X +6 more
europepmc +5 more sources
RNA-Puzzles Round IV:3D structure predictions of four ribozymes and two aptamers [PDF]
, 2020 International audienceRNA-Puzzles is a collective endeavor dedicated to the advancement and improvement of RNA 3D structure prediction. With agreement from crystallographers, the RNA structures are predicted by various groups before the publication of ...
Adamiak, Ryszard W. +49 more
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Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
, 2020 Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
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RNA catalysis in model protocell vesicles. [PDF]
, 2005 We are engaged in a long-term effort to synthesize chemical systems capable of Darwinian evolution, based on the encapsulation of self-replicating nucleic acids in self-replicating membrane vesicles.
Chen, Irene A +2 more
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A modular and extensible RNA-based gene-regulatory platform for engineering cellular function [PDF]
, 2007 Engineered biological systems hold promise in addressing pressing human needs in chemical processing, energy production, materials construction, and maintenance and enhancement of human health and the environment. However, significant advancements in our
Smolke, Christina D., Win, Maung Nyan
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Biophysical and biochemical investigations of RNA catalysis in the hammerhead ribozyme [PDF]
, 1999 1. How do ribozymes work? 241 2. The hammerhead RNA as a prototype ribozyme 242 2.1 RNA enzymes 242 2.2 Satellite self-cleaving RNAs 242 2.3 Hammerhead RNAs and hammerhead ribozymes 244 3.
Scott, William G
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A Faster Triphosphorylation Ribozyme. [PDF]
, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Akoopie, Arvin +2 more
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Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity. [PDF]
, 2010 The ability to generate RNA aptamers for synthetic biology using in vitro selection depends on the informational complexity (IC) needed to specify functional structures that bind target ligands with desired affinities in physiological concentrations of ...
Carothers, James M +4 more
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Specificity of hammerhead ribozyme cleavage. [PDF]
The EMBO Journal, 1996 To be effective in gene inactivation, the hammerhead ribozyme must cleave a complementary RNA target without deleterious effects from cleaving non-target RNAs that contain mismatches and shorter stretches of complementarity. The specificity of hammerhead cleavage was evaluated using HH16, a well-characterized ribozyme designed to cleave a target of 17 ...
Daniel Herschlag +2 more
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Crystals of a hammerhead ribozyme.
Journal of Biological Chemistry, 1993 Hammerhead ribozyme-inhibitor complexes consisting of an RNA "enzyme" strand and a DNA "inhibitor" strand have been crystallized in four different crystal forms. The crystal form that is most attractive for structure determination diffracts to approximately 3.2 A resolution; it is trigonal, space group P3(1)21 or enantiomorph, a = 92.5 A, c = 185.0 A.
C DeLuca-Flaherty +3 more
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