Results 231 to 240 of about 19,908 (281)

Cytotoxic Hammerhead Ribozymes

Antisense and Nucleic Acid Drug Development, 1999
Small catalytic RNA molecules of the hammerhead ribozyme type were found to have cytotoxic effects unrelated to their intended activity. An expression library of ribozyme sequence variants was constructed in a recA-deficient strain of Escherichia coli such that individual library members differed in regions designed to form base pairs with human ...
Xilin Zhao, Sheau Wen Jeng, Jian Ying Wang, Ruth Levitz, Yuzhi Dong, Jing Wa Wang, Chang Rung Chen, Chang Rung Chen, Karl Drlica, Jianfeng Zhou, Tao Lu   +10 more
openaire   +3 more sources

Hammerhead ribozyme engineering

Current Opinion in Structural Biology, 1996
Of all the catalytic RNAs the hammerhead ribozyme is the most chemically modified and structurally studied. Such studies have resulted in improvements in the nuclease resistance of ribozymes, reductions in their size, and improvements in their catalytic efficiency.
Leonid Beigelman, James McSwiggen, Nassim Usman   +2 more
openaire   +3 more sources

Cooperative Interactions in the Hammerhead Ribozyme Drive pKa Shifting of G12 and Its Stacked Base C17.

Biochemistry, 2017
General acid-base catalysis is a key mechanistic strategy in protein and RNA enzymes. Ribozymes use hydrated metal ions, nucleobases, and organic cofactors to carry this out.
Erica A. Frankel, Christopher A. Strulson, C. Keating, P. Bevilacqua   +3 more
semanticscholar   +1 more source

An Allosteric Hammerhead Ribozyme

Nature Biotechnology, 1995
We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate. The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded ...
Helena Porta, Paul M. Lizardi
openaire   +3 more sources

The Hammerhead Ribozyme Structure Brought in Line [PDF]

ChemBioChem, 2006
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Christian Hammann, Rita Przybilski
openaire   +3 more sources

Photochemical hammerhead ribozyme activation

Bioorganic & Medicinal Chemistry Letters, 2006
We report the light-activation of allosteric cis and trans acting ribozymes via decaging of a small organic molecule ligand. To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function.
Douglas D. Young, Alexander Deiters
openaire   +3 more sources

Allosteric Hammerhead Ribozyme TRAPs

Biochemistry, 2002
A new mode of allosteric regulation of nucleic acid enzymes is described and shown to operate effectively with hammerhead ribozymes. In the "TRAP" design (for targeted ribozyme-attenuated probe), a 3' terminal "attenuator" anneals to conserved bases in the catalytic core to form the "off" state of the ribozyme.
Nicole D. S. Ozerova, Donald H. Burke, Marit Nilsen-Hamilton   +2 more
openaire   +3 more sources

Prediction of hammerhead ribozyme intracellular activity with the catalytic core fingerprint.

Biochemical Journal, 2013
Hammerhead ribozyme is a versatile tool for down-regulation of gene expression in vivo. Owing to its small size and high activity, it is used as a model for RNA structure-function relationship studies.
Marta M Gabryelska, E. Wyszko, M. Szymanski, M. Popenda, J. Barciszewski   +4 more
semanticscholar   +1 more source

Folding and Activity of the Hammerhead Ribozyme

ChemBioChem, 2002
AbstractFor Abstract see ChemInform Abstract in Full Text.
David M.J. Lilley, Christian Hammann
openaire   +4 more sources

Small, Efficient Hammerhead Ribozymes

Molecular Biotechnology, 2000
The hammerhead ribozyme is able to cleave RNA in a sequence-specific manner. These ribozymes are usually designed with four basepairs in helix II, and with equal numbers of nucleotides in the 5' and 3' hybridizing arms that bind the RNA substrate on either side of the cleavage site.
Maxine J. McCall, Philip Hendry, Trevor Lockett, Glenn S. Brown, Jason Conaty, Alain A. Mir   +5 more
openaire   +3 more sources