Results 251 to 260 of about 19,908 (281)
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Kinetics of intermolecular cleavage by hammerhead ribozymes
Biochemistry, 1992The hammerhead catalytic RNA effects cleavage of the phosphodiester backbone of RNA through a transesterification mechanism that generates products with 2'-3'-cyclic phosphate and 5'-hydroxyl termini. A minimal kinetic mechanism for the intermolecular hammerhead cleavage reaction includes substrate binding, cleavage, and product release. Elemental rate
Olke C. Uhlenbeck, Martha J. Fedor
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Identification and characterization of a highly efficient anti-HIV pol hammerhead ribozyme.
Oligonucleotides, 2009The aim of this study was to identify and to characterize a highly active anti-HIV ribozyme. Therefore, the genome of HIV-1 IIIb was screened for not yet addressed GUC triplets within highly conserved sequences.
Thea Müller-Kuller +4 more
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Hammerhead Ribozyme-Based Target Discovery
2006With the accumulation of vast amounts of data as a result of the sequencing of the human genome, it is necessary to identify human genes that are involved in various cellular, developmental, and disease-related processes and to clarify their functions and potential utility as targets in the treatment of disease.
Kazunari Taira, Masayuki Sano
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Application of hammerhead ribozymes in filamentous fungi
Journal of Microbiological Methods, 2006Metabolic engineering in filamentous fungi is a emerging field of research as many fungi produce high value primary and secondary metabolites. Ribozyme technology can be used as a tool for metabolic engineering to influence metabolic pathways and to knock down the expression of specific genes of interest.
Vera Meyer, Ulf Stahl, Dirk Mueller
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Cobalt Hexammine Inhibition of the Hammerhead Ribozyme
Biochemistry, 2000The effects of Co(NH(3))(6)(3+) on the hammerhead ribozyme are analyzed using several techniques, including activity measurements, electron paramagnetic resonance (EPR), and circular dichroism (CD) spectroscopies and thermal denaturation studies. Co(NH(3))(6)(3+) efficiently displaces Mn(2+) bound to the ribozyme with an apparent dissociation constant ...
Victoria J. DeRose, Thomas E. Horton
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Structure—function studies of the hammerhead ribozyme
Current Opinion in Chemical Biology, 1997Elucidation of the catalytic mechanism and structure-function relationship studies of the hammerhead ribozyme continue to be an area of intensive research. A combination of diverse approaches, such as X ray crystallography, spectral studies, chemical modifications, sequence variations and kinetic analyses, have provided valuable insight into the ...
Fritz Eckstein +2 more
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Mechanistic Studies on Hammerhead Ribozymes
1996Catalytic RNAs include group I and II introns; the RNA subunit of RNase P; hammerhead, hairpin, and hepatitis delta virus ribozymes; and ribosomal RNA (Altman 1989; Cech 1989; Michel et al. 1989; Noller et al. 1992; Symons 1992; Bratty et al. 1993; Gesteland and Atkins 1993).
P. K. R. Kumar +3 more
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Effects of Cosolvents on the Folding and Catalytic Activities of the Hammerhead Ribozyme
ChemBioChem, 2015S. Nakano +4 more
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cis-Acting 5' hammerhead ribozyme optimization for in vitro transcription of highly structured RNAs.
Methods in molecular biology, 2014M. Meyer, B. Masquida
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