Results 31 to 40 of about 6,878 (169)

How RNA acts as a nuclease:some mechanistic comparisons in the nucleolytic ribozymes [PDF]

, 2017
Recent structural and mechanistic studies have shed considerable light on the catalytic mechanisms of nucleolytic ribozymes. The discovery of several new ribozymes in this class has now allowed comparisons to be made, and the beginnings of mechanistic ...
Lilley, David M. J.
core   +2 more sources

The Structure, Function and Application of the Hammerhead Ribozyme [PDF]

European Journal of Biochemistry, 1997
The hammerhead ribozyme is one of the smallest ribozymes known and catalyses the site‐specific hydrolysis of a phosphodiester bond. This small ribozyme is of interest for two reasons. It offers a convenient system to study the structure/function relationship of a nucleotide sequence, and is a potential vehicle for the inhibition of gene expression. The
Fritz Eckstein, Klara R. Birikh, Paul A. Heaton   +2 more
openaire   +3 more sources

Machine learning‐augmented molecular dynamics simulations (MD) reveal insights into the disconnect between affinity and activation of ZTP riboswitch ligands

Angewandte Chemie International Edition, Accepted Article.
The challenge of targeting RNA with small molecules necessitates a better understanding of RNA‐ligand interaction mechanisms. However, the dynamic nature of nucleic acids, their ligand‐induced stabilization, and how conformational changes influence gene expression pose significant difficulties for experimental investigation.
Christopher Fullenkamp, Shams Mehdi, Christopher Jones, Logan Tenney, Patricio Pichling, Peri R. Prestwood, Adrian R. Ferré-D'Amaré, Pratyush Tiwary, John Schneekloth   +8 more
wiley   +1 more source

Exploring the Potential and Advancements of Circular RNA Therapeutics

Exploration, EarlyView.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang, Lianqing Wang, Chunbo Dong, Jialong Liu, Guoxin Cui, Shan Gao, Zhida Liu   +6 more
wiley   +1 more source

Lipid vesicles chaperone an encapsulated RNA aptamer. [PDF]

, 2018
The organization of molecules into cells is believed to have been critical for the emergence of living systems. Early protocells likely consisted of RNA functioning inside vesicles made of simple lipids.
Chen, Irene A, Saha, Ranajay, Verbanic, Samuel   +2 more
core   +1 more source

Selective Nonenzymatic Formation of Biologically Common RNA Hairpins

Angewandte Chemie, Volume 137, Issue 5, January 27, 2025.
Template‐free RNA loop‐closing ligation selectively generates RNA tetraloops that are prevalent in biology, as demonstrated by high‐throughput sequencing. Utilizing high‐yield overhang sequences enables the efficient assembly of functional RNAs. Abstract The prebiotic formation of RNA building blocks is well‐supported experimentally, yet the emergence ...
Long‐Fei Wu, Junting Zhang, Robert Cornwell‐Arquitt, David A. Hendrix, Aleksandar Radakovic, Jack W. Szostak   +5 more
wiley   +2 more sources

A split ribozyme system for in vivo plant RNA imaging and genetic engineering

Plant Biotechnology Journal, Volume 23, Issue 5, Page 1640-1649, May 2025.
Summary RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops
Yang Liu, Ruchika Rajput, Md Torikul Islam, Ilenne Del Valle, Tao Yao, Rekha Agrawal, Brandon A. Boone, Carrie A. Eckert, Paul E. Abraham, Jin‐Gui Chen, Gerald A. Tuskan, Xiaohan Yang   +11 more
wiley   +1 more source

Role of tautomerism in RNA biochemistry [PDF]

, 2015
Heterocyclic nucleic acid bases and their analogs can adopt multiple tautomeric forms due to the presence of multiple solvent-exchangeable protons.
Essigmann, John M., Fedeles, Bogdan I., Singh, Vipender   +2 more
core   +1 more source

Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst?

ChemBioChem, Volume 26, Issue 10, May 27, 2025.
Rational design is a potent strategy for developing ribozymes with advanced functionalities. Engineered variants of the hairpin ribozyme impressively demonstrate how function can be tuned through structural manipulation. The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention ...
Constanze Ebermann, Sabine Müller
wiley   +1 more source

Viroids: Survivors from the RNA World? [PDF]

, 2014
Because RNA can be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before DNA and proteins, which eventually assumed these roles and relegated RNA to intermediate functions.
Flores, Ricardo, Gago, Selma, Sanjuán, Rafael, Serra, Pedro   +3 more
core   +1 more source