Results 31 to 40 of about 19,908 (281)

Crystals of a hammerhead ribozyme.

open access: hybridJournal of Biological Chemistry, 1994
Hammerhead ribozyme-inhibitor complexes consisting of an RNA "enzyme" strand and a DNA "inhibitor" strand have been crystallized in four different crystal forms. The crystal form that is most attractive for structure determination diffracts to approximately 3.2 A resolution; it is trigonal, space group P3(1)21 or enantiomorph, a = 92.5 A, c = 185.0 A.
H.W. Pley   +3 more
openalex   +4 more sources

In vitro activity of minimised hammerhead ribozymes [PDF]

open access: greenNucleic Acids Research, 1995
A number of minimised hammerhead ribozymes (minizymes) which lack stem II have been kinetically characterised. These minizymes display optimal cleavage activity at temperatures around 37 degrees C. The cleavage reactions of the minizymes are first order in hydroxide ion concentration up to around pH 9.3 above which the cleavage rate constants decline ...
Philip Hendry   +3 more
openalex   +5 more sources

Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]

open access: yes, 2020
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P   +3 more
core   +1 more source

Cryoenzymology of the hammerhead ribozyme [PDF]

open access: yesRNA, 1998
The technique of cryoenzymology has been applied to the hammerhead ribozyme in an attempt to uncover a structural rearrangement step prior to cleavage. Several cryosolvents were tested and 40% (v/v) methanol in water was found to perturb the system only minimally.
Olke C. Uhlenbeck   +2 more
openaire   +3 more sources

The unforeseeable hammerhead ribozyme [PDF]

open access: yesF1000 Biology Reports, 2009
Despite its small size, the complex behavior of the hammerhead ribozyme keeps surprising us, even more than 20 years after its discovery. Here, we summarize recent developments in the field, in particular the discovery of the first split hammerhead ribozyme.
Christian Hammann, Eric Westhof
openaire   +3 more sources

Hammerhead ribozyme kinetics [PDF]

open access: yesRNA, 1998
The hammerhead ribozyme is a small RNA motif that self cleaves at a specific phosphodiester bond to produce 2′,3′ cyclic phosphate and 5′ hydroxyl termini (Hutchins et al., 1986; Forster & Symons, 1987a). The secondary structure of the hammerhead consists of three helices of arbitrary sequence and length (designated I, II, and III) that intersect at 15
Tracy K. Stage-Zimmermann   +1 more
openaire   +3 more sources

Identification of hammerhead ribozymes in all domains of life reveals novel structural variations. [PDF]

open access: yesPLoS Computational Biology, 2011
Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known ...
Jonathan Perreault   +6 more
doaj   +1 more source

Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions

open access: yesLife, 2022
The acquisition of functions via the elongation of nucleotides is an important factor in the development of the RNA world. In our previous study, we found that the introduction of complementary seven-membered kissing loops into inactive R3C ligase ...
Hiromi Mutsuro-Aoki, Koji Tamura
doaj   +1 more source

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