Spiegelzymes: sequence specific hydrolysis of L-RNA with mirror image hammerhead ribozymes and DNAzymes. [PDF]
PLoS ONE, 2013 In this manuscript we describe for the first time mirror image catalytic nucleic acids (Spiegelzymes), which hydrolyze sequence specifically L-ribonucleic acid molecules.
Eliza Wyszko +5 more
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Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
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Enhanced product stability in the hammerhead ribozyme. [PDF]
Biochemistry, 2010 Shepotinovskaya I, Uhlenbeck OC.
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Cryoenzymology of the hammerhead ribozyme [PDF]
RNA, 1998 The technique of cryoenzymology has been applied to the hammerhead ribozyme in an attempt to uncover a structural rearrangement step prior to cleavage. Several cryosolvents were tested and 40% (v/v) methanol in water was found to perturb the system only minimally.
A L, Feig, G E, Ammons, O C, Uhlenbeck
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Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time
BioTechniques, 2000 Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh +4 more
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Functional Analysis of Histidine-Dependent Self-Cleaving RNAs Obtained by In Vitro Selection. [PDF]
ChembiochemRibozymes can be nucleic acid medicines given their catalytic activity. We attempted to obtain functional RNAs that mimic ribonuclease A catalytic mechanisms. Functional RNAs with high histidine specificity were obtained, suggesting that histidine acts in active structure formation.
Sakimoto N +8 more
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Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.). [PDF]
Plant JSUMMARY Genome editing using the CRISPR/Cas system enables rapid and efficient plant breeding by directly introducing desired traits into elite lines within a short time frame. However, challenges associated with conventional Agrobacterium tumefaciens‐mediated transformation and regeneration have limited gene editing in pepper (Capsicum annuum L.).
Kang B +6 more
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Rolling circle RNA synthesis catalyzed by RNA
eLife, 2022 RNA-catalyzed RNA replication is widely considered a key step in the emergence of life’s first genetic system. However, RNA replication can be impeded by the extraordinary stability of duplex RNA products, which must be dissociated for re-initiation of ...
Emil Laust Kristoffersen +3 more
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Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
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Cold Denaturation of the Hammerhead Ribozyme [PDF]
Journal of the American Chemical Society, 2002 Cold denaturation is a thermodynamic phenomenon resulting from a difference in the heat capacities, DeltaCp, of the folded and unfolded states of a macromolecule. Whereas this phenomenon has been extensively studied in proteins, it has been thought not to occur in nucleic acids due to a negligible DeltaCp of folding.
Peter J, Mikulecky, Andrew L, Feig
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