Results 51 to 60 of about 19,908 (281)
The roles of cosolutes on the hammerhead ribozyme activity [PDF]
Nucleic Acids Symposium Series, 2006 The hammerhead ribozyme is often used for gene regulations in a cell. One of the major differences between in vitro and in cell conditions is the molecular crowding. However, the influence of crowding conditions on the ribozyme activity is still unclear. Here, we investigated the activity of the hammerhead ribozyme under molecular crowding condition by
Hisae Karimata +2 more
openalex +4 more sources
Molecular Therapy: Nucleic Acids, 2022 The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA ...
Tomasz Czapik +3 more
doaj
Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time
BioTechniques, 2000 Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh +4 more
doaj +1 more source
Intronic hammerhead ribozymes in mRNA biogenesis [PDF]
bchm, 2012 Abstract Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes
García Robles, Inmaculada +2 more
openaire +5 more sources
Distribution of hammerhead and hammerhead-like RNA motifs through the GenBank [PDF]
, 2000 Hammerhead ribozymes previously were found in satellite RNAs from plant viroids and in repetitive DNA from certain species of newts and schistosomes. To determine if this catalytic RNA motif has a wider distribution, we decided to scrutinize the GenBank ...
Bourdeau, V. +4 more
core +2 more sources
A Faster Triphosphorylation Ribozyme. [PDF]
, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Akoopie, Arvin +2 more
core +4 more sources
Self-assembly Controls Self-cleavage of HHR from ASBVd (−): a Combined SANS and Modeling Study [PDF]
, 2016 International audienceIn the Avocado Sunblotch Viroid (ASBVd: 249-nt) from the Avsunviroidae family, a symmetric rolling-circle replication operates through an autocatalytic mechanism mediated by hammerhead ribozymes (HHR) embedded in both polarity ...
Leclerc, Fabrice +5 more
core +3 more sources
Specificity of hammerhead ribozyme cleavage. [PDF]
The EMBO Journal, 1996 To be effective in gene inactivation, the hammerhead ribozyme must cleave a complementary RNA target without deleterious effects from cleaving non-target RNAs that contain mismatches and shorter stretches of complementarity. The specificity of hammerhead cleavage was evaluated using HH16, a well-characterized ribozyme designed to cleave a target of 17 ...
Daniel Herschlag +2 more
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Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
doaj +1 more source
Hammerhead ribozymes going viral [PDF]
Genome Biology, 2016 An association between hammerhead ribozymes and non-autonomous, long terminal repeat retrotransposons is uncovered in plants, shedding light on the biological function of genomically encoded ribozymes.
openaire +3 more sources