Results 51 to 60 of about 16,494 (236)
Hammerhead ribozymes going viral [PDF]
Genome Biology, 2016 An association between hammerhead ribozymes and non-autonomous, long terminal repeat retrotransposons is uncovered in plants, shedding light on the biological function of genomically encoded ribozymes.
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Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus
Virology Journal, 2017 Background Recovery of recombinant negative-stranded RNA viruses from cloned cDNAs is an inefficient process as multiple viral components need to be delivered into cells for reconstitution of infectious entities.
Shasha Qian +4 more
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Intracellular Selection of Theophylline-Sensitive Hammerhead Aptazyme
Molecular Therapy: Nucleic Acids, 2020 Hammerhead ribozyme-based aptazyme (HHAz), inheriting the advantages of small size and high efficiency from the RNA-cleaving ribozyme and the specific recognition ability of aptamers to specific targets, exhibits the huge potential to be a transgene ...
Qinlin Pu +8 more
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Langmuir, 2015 Compared with proteins, the relationship between structure, dynamics, and function of RNA enzymes (known as ribozymes) is far less well understood, despite the fact that ribozymes are found in many organisms and are often conceived as "molecular fossils"
J. B. Swadling +3 more
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A structural analysis of
BMC Bioinformatics, 2007 Background Ribozymes are small catalytic RNAs that possess the dual functions of sequence-specific RNA recognition and site-specific cleavage. Trans-cleaving ribozymes can inhibit translation of genes at the messenger RNA (mRNA) level in both eukaryotic ...
Schneider Erasmus +5 more
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Modified Hammerhead Ribozymes as Potential Therapeutics [PDF]
Nucleosides and Nucleotides, 1998 Abstract Hammerhead ribozymes were modified in the 2′-position by flouro-, amino-, deoxy-, O-methyl- and methoxyethoxy-groups to stabilize against degradations. They were tested for their ability to cut specifically oncogenic N-ras RNA in vitro. Exogenous delivery by lipofection as well as viral vector mediated transfection showed comparable results in
Engels, Joachim W. +4 more
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Parasites & Vectors, 2019 Background Giardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly ...
Jing-Tong Zheng +10 more
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Catalytic Diversity of Extended Hammerhead Ribozymes [PDF]
Biochemistry, 2008 Chimeras of the well-characterized minimal hammerhead 16 and nine extended hammerheads derived from natural viroids and satellite RNAs were constructed with the goal of assessing whether their very different peripheral tertiary interactions modulate their catalytic properties.
Irina V, Shepotinovskaya +1 more
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Efficient large-scale preparation and purification of short single-stranded RNA oligonucleotides
BioTechniques, 2016 Sequence-specific RNA recognition by RNA-binding proteins plays a crucial role in the post-translational regulation of gene expression. Biophysical and biochemical studies help to unravel the principles of sequence-specific RNA recognition, but the ...
Maria Zlobina +4 more
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RNA folding and catalysis mediated by iron (II). [PDF]
PLoS ONE, 2012 Mg²⁺ shares a distinctive relationship with RNA, playing important and specific roles in the folding and function of essentially all large RNAs. Here we use theory and experiment to evaluate Fe²⁺ in the absence of free oxygen as a replacement for Mg²⁺ in
Shreyas S Athavale +13 more
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