Results 51 to 60 of about 6,878 (169)

Identification of Hammerhead Ribozymes in All Domains of Life Reveals Novel Structural Variations [PDF]

, 2011
Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known ...
A Khvorova, A Laferriere, A Oren, A Oren, AA Rojas, AC Forster, AC Forster, Adam Roth, AR Kore, C Seehafer, CH Webb, CJ Hutchins, DA Benson, DE Draper, DE Draper, DE Ruffner, DG Eickbush, EA Davidson, EE Regulski, EP Nawrocki, F Eckstein, G Ferbeyre, G Ferbeyre, G Lennon, GA Prody, Gerardo Ferbeyre, H Bolhuis, HG Kang, J Tang, J Tang, JA Esteban, JA Nelson, JB Murray, JC Cochrane, JH Christian, JL Siefert, Jonathan Perreault, K Salehi-Ashtiani, K Salehi-Ashtiani, KJ Hertel, L Martinsen, L Rocheleau, LL Daniels, LM Epstein, M De la Pena, M De la Pena, M De la Pena, M Martick, M Martick, M Safran, MD Canny, NB Leontis, Olivia Popescu, Pascal Chartrand, PP Gardner, R Przybilski, R Przybilski, R Przybilski, RM Jimenez, Ronald R. Breaker, RR Breaker, S Ambros, S Gago, S Graf, SD Nuttall, SL Tang, T Torsvik, TJ Macke, WC Winkler, WG Scott, WL DeLano, Wyeth W. Wasserman, Y Tan, Y Tokumoto, Z Weinberg, Z Weinberg, Z Weinberg, Z Yao, Zasha Weinberg   +78 more
core   +3 more sources

Solvent Structure and Hammerhead Ribozyme Catalysis

Chemistry & Biology, 2008
Although the hammerhead ribozyme is regarded as a prototype for understanding RNA catalysis, the mechanistic roles of associated metal ions and water molecules in the cleavage reaction remain controversial. We have investigated the catalytic potential of observed divalent metal ions and water molecules bound to a 2 A structure of the full-length ...
William G. Scott, William G. Scott, Tai-Sung Lee, Darrin M. York, Monika Martick   +4 more
openaire   +4 more sources

Rolling-circle replication of viroids, viroid-like satellite RNAs and hepatitis delta virus: variations on a theme [PDF]

, 1999
Viroids and viroid-like satellite RNAs from plants, and the human hepatitis delta virus (HDV) RNA share some properties that include small size, circularity and replication through a rolling-circle mechanism.
Flores, Ricardo, Grubb, Douglas, Elleuch, Amine, Nohales, María-Ángeles, Delgado, Sonia, Gago, Selma   +5 more
core   +1 more source

Modulating RNA structure and catalysis: lessons from small cleaving ribozymes [PDF]

, 2009
RNA is a key molecule in life, and comprehending its structure/function relationships is a crucial step towards a more complete understanding of molecular biology.
A Berzal-Herranz, A Ferguson, A Hampel, A Hampel, A Jenne, A Ke, A Kertsburg, A Khvorova, A Nehdi, A Nehdi, A Peracchi, A Roth, A Serganov, AA Andersen, AA Rojas, AC Forster, AL Feig, AM Jose, AR Ferré-D’Amaré, AR Ferré-D’Amaré, AR Ferré-D’Amaré, AT Perrotta, AT Perrotta, AT Perrotta, B Onoa, BJ Saville, BM Chowrira, C Reymond, C Reymond, Cedric Reymond, CJ Hutchins, D Hearschlag, D Lévesque, D Lévesque, D Strohbach, DA Harris, DB McKay, DD Young, DE Draper, DH Burke, DJ Earnshaw, DJ Klein, DM Lilley, DM Lilley, DM Lilley, DY Wang, DY Wang, DY Wang, E Jankowsky, EA Alemán, EL Bertrand, EM Moody, F Nishikawa, F Nishikawa, FD Jones, G Ferbeyre, G Pljevaljcic, GA Prody, GA Robichaud, GA Soukup, GA Soukup, GA Soukup, H Bhaskaran, H Porta, HC Guo, HW Lee, HW Pley, IH Shih, J Duhamel, J Fiore, J Lipfert, J Ouellet, J Tang, J Tang, JA Nelson, JB Murray, JB Murray, JC Cochrane, JC Cochrane, JC Cochrane, JD Beaudoin, JE Barrick, JE Olive, Jean-Denis Beaudoin, Jean-Pierre Perreault, JM Buzayan, JM Buzayan, K Fiola, K Fiola, K Salehi-Ashtiani, KA Marshall, KF Blount, KH Link, L Citti, L Jaeger, LJ Bergeron, LJ Bergeron, LM Epstein, M Amarzguioui, M Araki, M Koizumi, M Kuciak, M la Peña De, M Martick, M Meli, M Sioud, M Wieland, M Zivarts, MD Been, MD Been, MD Canny, MJ Fedor, MJ Fedor, MN Win, NB Leontis, NK Vaish, P Deschênes, PB Rupert, R Russell, RA Collins, RA Collins, RH Symons, S Ananvoranich, S Cho, S Nakano, S Vauléon, S Verma, SE Butcher, SH Najafi-Shoushtari, SL Hiley, T Rastogi, TE Horton, TJ McCarthy, TJ Wilson, TK Stage, TS Wadkins, WC Winkler, WG Scott, WJ Greenleaf, Y Komatsu, Y Komatsu, Y Komatsu, Y Komatsu, Y Tanaka, YA Suh, Z Tsuchihashi, Z Zhuang, ZS Huang   +147 more
core   +2 more sources

Elucidating Evolutionary Mechanisms and Variants of the Hammerhead Ribozyme Using In Vitro Selection

ChemBioChem, Volume 25, Issue 21, November 4, 2024.
We generated single‐, double‐, and triple‐mutation libraries to explore the sequence requirements and evolution of a full‐length hammerhead ribozyme. We identified many hammerhead variants, some of which possess mutations at previously considered essential nucleotides. We also present a novel methodological approach for evaluating the sequence‐function
Jake Brill, Connor Nurmi, Yingfu Li
wiley   +1 more source

Nucleozymes [PDF]

, 2004
Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts.
Cedergren, Robert J., Perreault, Jean-Pierre, Rich, Alexander, Usman, Nassim, Yang, Jing-Hua   +4 more
core   +1 more source

Specificity of novel allosterically trans- and cis-activated connected maxizymes that are designed to suppress BCR-ABL expression [PDF]

, 1999
Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22.
Hamada, Makiko, Kuwabara, Tomoko, Nakayama, Aya, Taira, Kazunari, Warashina, Masaki   +4 more
core   +1 more source

Unlocking the Potential of Chemically Modified Nucleic Acid Therapeutics

Advanced Therapeutics, Volume 7, Issue 11, November 2024.
The advancements in nucleic acid therapeutics are highlighted, focusing on chemical modifications and bioconjugation techniques that enhance stability, binding affinity, and targeted delivery. Recent literature and clinical applications emphasize the role of backbone, ribose, base modifications, and bioconjugation strategies like N‐acetylgalactosamine (
Jingjing Gao, Bhingaradiya Nutan, Dorra Gargouri, Nishkal D. Pisal, Vy Do, Muhammad Zubair, Hommam Alanzi, Hiqui Wang, Dongtak Lee, Nitin Joshi, Aman Ullah   +10 more
wiley   +1 more source

A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity [PDF]

, 2012
Recent advances have demonstrated the use of RNA-based control devices to program sophisticated cellular functions; however, the efficiency with which these devices can be quantitatively tailored has limited their broader implementation in cellular ...
Chang, Andrew L., Kennedy, Andrew B., Liang, Joe C., Smolke, Christina D.   +3 more
core   +4 more sources

In vitro activity of minimised hammerhead ribozymes

Nucleic Acids Research, 1995
A number of minimised hammerhead ribozymes (minizymes) which lack stem II have been kinetically characterised. These minizymes display optimal cleavage activity at temperatures around 37 degrees C. The cleavage reactions of the minizymes are first order in hydroxide ion concentration up to around pH 9.3 above which the cleavage rate constants decline ...
Fernando S. Santiago, Philip Hendry, Philip A. Jennings, Maxine J. Mccall   +3 more
openaire   +4 more sources