A Web Service for Automated Design of Multiple Types of Ribozymes Targeting RNA: from minimal hammerhead to aptazymes [PDF]
, 2023 Sabrine Najeh +7 more
openalex +1 more source
Development of a Chimeric DNA-RNA Hammerhead Ribozyme Targeting SARS Virus
Intervirology, 2009 Objective: Severe acute respiratory syndrome (SARS) is a severe pulmonary infectious disease caused by a novel coronavirus. To develop an effective and specific medicine targeting the SARS-coronavirus (CoV), a chimeric DNA-RNA hammerhead ribozyme was ...
A. Fukushima +8 more
semanticscholar +1 more source
High‐Efficiency Genome Editing of Chlorella sp. by CRISPR/Cas9
Plant Biotechnology Journal, EarlyView.
Xinyu Cui +5 more
wiley +1 more source
RNA elements and their biotechnological applications in plants
New Phytologist, Volume 247, Issue 6, Page 2517-2537, September 2025.Summary Engineering of plants for improved traits and efficient heterologous protein production can be achieved by modifying or introducing cis‐ or trans‐acting RNA elements. The function of these elements depends not only on their nucleotide sequence but also on their highly dynamic higher order structures.
Filip Lastovka +3 more
wiley +1 more source
Schistosome Satellite DNA Encodes Active Hammerhead Ribozymes [PDF]
Molecular and Cellular Biology, 1998 Using a computer program designed to search for RNA structural motifs in sequence databases, we have found a hammerhead ribozyme domain encoded in the Smalpha repetitive DNA of Schistosoma mansoni. Transcripts of these repeats are expressed as long multimeric precursor RNAs that cleave in vitro and in vivo into unit-length fragments. This RNA domain is
G, Ferbeyre, J M, Smith, R, Cedergren
openaire +2 more sources
Discovery of Viroids and Viroid‐Like RNAs in Plants
Plant Biotechnology Journal, EarlyView.
Zhiyou Xuan +15 more
wiley +1 more source
Engineering and comparison of cas12a‐based genome editing systems in plants
The Plant Journal, Volume 123, Issue 5, September 2025.SUMMARY While Cas9 and Cas12a are both RNA‐guided endonucleases used for genome editing, only Cas12a is able to process pre‐crRNA via its additional ribonuclease activity. This feature reduces the complexity of Cas12a versus Cas9‐based genome editing systems thus providing an attractive alternative for generating site‐specific mutations in plants. Here
Martin Bircheneder, Martin Parniske
wiley +1 more source
Exploring the Potential and Advancements of Circular RNA Therapeutics
Exploration, Volume 5, Issue 4, August 2025.Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang +6 more
wiley +1 more source
Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function.
World Journal of Gastroenterology, 2009 AIM To determine the effect of hammerhead ribozyme targeting connective tissue growth factor (CCN2) on human hepatic stellate cell (HSC) function. METHODS CCN2 hammerhead ribozyme cDNA plus two self-cleaving sequences were inserted into pTriEx2 to ...
R. Gao, D. Brigstock
semanticscholar +1 more source
Unlocking the Synthetic Potential of Yarrowia lipolytica: Innovating Gene Expression Tools
Microbial Biotechnology, Volume 18, Issue 8, August 2025.As an important industrial microbial chassis, the development of gene expression tools for Yarrowia lipolytica is of great significance for advancing its industrial application process. This opinion article highlights the recent developments in promoter engineering and gene editing systems, current challenges and future prospects.
Xiaoqin Liu, Qingsheng Qi
wiley +1 more source