Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Biotechnology Progress, EarlyView.Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Biotechnology Progress, EarlyView.Abstract A rapid assessment of manufacturability for drug candidates is crucial for advancing a prospective biotherapeutic from a candidate to a bulk drug substance. A lot‐to‐lot approach to manufacturability is adopted where each biologic batch is assessed for manufacturability as a bulk, unfractionated pool.
Solomon Isu +6 more
wiley +1 more source
Inhalable Nanobody (PiN-21) prevents and treats SARS-CoV-2 infections in Syrian hamsters at ultra-low doses [PDF]
, 2021 Sham Nambulli +9 more
openalex +1 more source
Performance Comparison between Semi‐Automated and Manual Cell Counting for Animal Cell Culture
Chemie Ingenieur Technik, EarlyView.Following ISO 20391‐2:2019 guidelines, manual and semi‐automated cell counting performances were compared using validation parameters from ICH Q2(R1). Specificity, linearity, and repeatability served as key validation parameters in the evaluation of total cell density and cell viability of CHO‐K1 cells using trypan blue exclusion staining.
Selina Uhlendorff +3 more
wiley +1 more source
Comparison of the genotoxic and apoptosis-inducing properties of ganciclovir and penciclovir in Chinese hamster ovary cells transfected with the thymidine kinase gene of herpes simplex virus-1: Implications for gene therapeutic approaches [PDF]
, 2000 R Thust +5 more
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The Canadian Journal of Chemical Engineering, EarlyView.Abstract On the centennial of higher education in Chemical Engineering in Mexico, it is pertinent to revisit the key stages that have contributed to its consolidation as a vital discipline for the nation's scientific and technological advancement. Although the initial mission of chemical engineering education was primarily oriented toward the training ...
Agustín López Munguía +3 more
wiley +1 more source
Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis
eLifeLeptospirosis is an emerging infectious disease caused by pathogenic Leptospira spp. Humans and some mammals can develop severe forms of leptospirosis accompanied by a dysregulated inflammatory response, which often results in death.
Xufeng Xie +5 more
doaj +1 more source
Electrorotation Studies of Baby Hamster Kidney Fibroblasts Infected with Herpes Simplex Virus Type 1 [PDF]
, 1999 Steffen Archer +2 more
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PharmVar GeneFocus: NAT2—Genetic Variation and Updated Nomenclature
Clinical Pharmacology &Therapeutics, EarlyView.The Pharmacogene Variation Consortium (PharmVar) provides nomenclature for the highly polymorphic human N‐acetyltransferase 2 (NAT2) gene. NAT2 metabolizes several clinically used drugs including isoniazid, hydralazine, amifampridine, procainamide, and sulfonamides such as dapsone, and also some highly carcinogenic arylamines.
Georgia Papanikolaou +14 more
wiley +1 more source
Zyflamend® reduces LTB 4 formation and prevents oral carcinogenesis in a 7,12-dimethylbenz[α]anthracene (DMBA)-induced hamster cheek pouch model [PDF]
, 2008 Peiying Yang +7 more
openalex +1 more source