Results 91 to 100 of about 1,935,322 (253)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Spin waves in the soft layer of exchange-coupled soft/hard bilayers
AIP Advances, 2016 The magnetic dynamical properties of the soft layer in exchange-coupled soft/hard bilayers have been investigated numerically using a one-dimensional atomic chain model.
Zheng-min Xiong +9 more
doaj +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
League-ally Speaking was a newsletter published by the Toledo League for the Hard of Hearing in Toledo, Ohio. Incorporated in 1920, the organization was also an agency of the Toledo Community Chest. In 1947 the organization changed its name to the Toledo
Toledo League for the Hard of Hearing
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Hard Disks on the Hyperbolic Plane [PDF]
, 2007 We examine a simple hard disk fluid with no long range interactions on the two-dimensional space of constant negative Gaussian curvature, the hyperbolic plane.
Kamien, Randall D., Modes, Carl D.
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Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
League-ally Speaking was a newsletter published by the Toledo League for the Hard of Hearing in Toledo, Ohio. Incorporated in 1920, the organization was also an agency of the Toledo Community Chest. In 1947 the organization changed its name to the Toledo
Toledo League for the Hard of Hearing
core
"Modes of governance in the EU" [PDF]
, 2007 ...it is the aim of this chapter to, first, clarify the concept of governance, second, highlight basic modes of governance and their role in the EU and, third, develop an explanatory framework for the emergence of specific modes of governance in the ...
Tömmel, Ingeborg.
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FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source