Results 111 to 120 of about 657 (145)
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l-Threonine dehydratase activity of axenically grown Hartmannella culbertsoni
Archiv f�r Mikrobiologie, 19720730 09 1. l-Threonine dehydratase (E.C. 4.2.1.16) activity in the cell-free extracts of the trophozoites of Hartmannella culbertsoni has been purified 20-fold. 2. The enzyme requires pyridoxal-5-phosphate for its activity and is not cold-labile.
M K, Raizada, V K, Rao
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The Physiology of Encystment of Hartmannella castellanii*
The Journal of Protozoology, 1969SYNOPSIS. Some aspects of the physiology of encystment of the soil amoeba Hartmannella castellanii in a replacement encystment medium consisting of 5 × 10‐2 M MgCl2 have been investigated. It is suggested that measurement of the cellulose produced during encystment in the synthesis of the cyst wall is a more reliable measure of the process than other ...
A. J. GRIFFITHS, D. E. HUGHES
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Antonie van Leeuwenhoek, 1997
Axenically and monoxenically grown Acanthamoeba castellanii, Acanthamoeba polyphaga and different isolates of Hartmannella vermiformis strains were examined by polyacrylamide isoelectric focusing in the pH range 3-10. Isoenzyme patterns of acid phosphatase (AP), propionyl esterase (PE), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glucose ...
P H, Weekers, J F, De Jonckheere
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Axenically and monoxenically grown Acanthamoeba castellanii, Acanthamoeba polyphaga and different isolates of Hartmannella vermiformis strains were examined by polyacrylamide isoelectric focusing in the pH range 3-10. Isoenzyme patterns of acid phosphatase (AP), propionyl esterase (PE), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glucose ...
P H, Weekers, J F, De Jonckheere
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Hartmannella: Growth controlling substances in culture medium
Protoplasma, 1970Growth inhibiting thermolabile factor(s) accumulate in some of the axenic media during growth ofHartmannella cultures. The physiological effectiveness of the factors depends in a not understood way on the concentration of cells in culture. The factor may be active antigenically.
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Serologic Changes Associated with the Encystment of Axenically Grown Hartmannella culbertsoni*
The Journal of Protozoology, 1972SYNOPSIS. Serologic reactions elicited by sonically ruptured trophic and cystic forms of Hartmannella culbertsoni were studied. The antigens of trophic amoebae reacted with their homologous rabbit antiserum showing multiple precipitin lines which could not be seen when the reacting antigens were treated with trypsin prior to application on the ...
M K, Raizada, K C, Saxena, C R, Murti
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Purification and properties of a bacteriolytic enzyme from a soil amoeba Hartmannella glebae
Archives of Biochemistry and Biophysics, 1969Abstract A 74-fold purification of the bacteriolytic enzyme from a soil amoeba, Hartmannella glebae grown on an agar lawn of Aerobacter aerogenes has been achieved by fractionation of the crude extract with acetone and chromatography on DEAE-cellulose. The optimal pH for lytic activity is 8.0.
Y C, Chung, J M, Upadhyay, F J, Garcia
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Cell Differentiation, 1975
During encystation of Hartmannella culbertsoni induced by taurine or epinephrine, 60-70% of the reserve glycogen is degraded. Glycogen phosphorylase is activated and glycogen synthetase is inhibited after 6-8 hr of exposure to the encystation medium.
A K, Verma, M K, Raizada
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During encystation of Hartmannella culbertsoni induced by taurine or epinephrine, 60-70% of the reserve glycogen is degraded. Glycogen phosphorylase is activated and glycogen synthetase is inhibited after 6-8 hr of exposure to the encystation medium.
A K, Verma, M K, Raizada
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Intracellular survival of Francisella tularensis in Hartmannella vermiformis
2010Francisella tularensis is a highly infectious, facultative intracellular bacterium and the causative agent of tularemia. The natural reservoir of the bacterium is largely unknown. Although it has been shown that protozoa may harbor it the reservoir in nature has not been definitely identified. In this study, the ability of F. tularensis subsp. novicida
Ožanič, Mateja, Šantić, Marina
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Experimental Cell Research, 1972
Abstract By ultrafiltration of conditioned synthetic medium from Hartmannella cultures through membranes of decreasing pore diameter, six fractions of large molecular substances were obtained. These fractions can be divided into two classes with different physiological properties (molecular weight above and below 30 000).
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Abstract By ultrafiltration of conditioned synthetic medium from Hartmannella cultures through membranes of decreasing pore diameter, six fractions of large molecular substances were obtained. These fractions can be divided into two classes with different physiological properties (molecular weight above and below 30 000).
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Extrinsic Requirements for Encystation by the Soil Amoeba, Hartmannella rhysodes*
The Journal of Protozoology, 1963SYNOPSIS. The environmental factors causing encystation in Hartmannella rhysodes, a small soil amoeba, appear to be an elevated osmotic pressure, Ca2+ and Mg2+, aeration and a decrease in carbon sources. After 36 hr under these conditions, amoebae harvested during active growth encyst. Nutrients essential for growth, other than carbon sources, have no
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