Results 151 to 160 of about 108,215 (261)

Development of Modular Geminivirus‐Based Vectors for High Cargo Expression and Gene Targeting in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT Viral vectors can be useful tools for expressing recombinant proteins as well as delivering gene‐editing machinery. Despite their utility, the development and subsequent optimisation of these tools is often a difficult and tedious process.
Matthew Neubauer, Katie Vollen, Chengsong Zhao, José T. Ascencio‐Ibáñez, Linda Hanley‐Bowdoin, Anna N. Stepanova, Jose M. Alonso   +6 more
wiley   +1 more source

Compact bacterial recombination complexes drive efficient kilobase-scale knock-in in mammalian cells. [PDF]

Nucleic Acids Res
Luo Y, Jiang Q, Qu Y, Li W, Liu R, Zhu Y, Xie Y, Jiang C, Chen C, Cong L, Han F, Bao J, Wang C.   +12 more
europepmc   +1 more source

Heterologous Production of Forskolin in Tobacco (Nicotiana tabacum) via Glandular Trichome Specific Engineering and Metabolic Flux Redirection

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plants are promising bioreactor for the sustainable and scalable production of high‐value natural bioactive compounds, because they can synthesise phytochemicals from CO2, light, water and minerals through their innate photosynthetic carbon assimilation machinery.
Xuan Sun, Xiuming Wu, Xun Jiang, Hongmei Huang, Aiguo Yang, Yiting Li, Zhenjun Fan, Aixia Chang, Changqing Yang   +8 more
wiley   +1 more source

Screening strategy to identify Cas9 variants with higher HDR activity based on diphtheria toxin. [PDF]

J Biomed Sci
Matsumoto D, Kubota K, Sato Y, Kato-Inui T, Nigorikawa K, Miyaoka Y, Nomura W.   +6 more
europepmc   +1 more source

Efficient Debanding Filtering for Inverse Tone Mapped High Dynamic Range Videos [PDF]

, 2020
Cosman, Pamela C, Song, Qing, Su, Guan-Ming   +2 more
core  

Improved Longan Genome Assembly Reveals Insights Into Flowering Mechanisms

Plant Biotechnology Journal, EarlyView.
ABSTRACT Longan is an exotic tropical fruit crop and exhibits off‐season flowering induced by potassium chlorate (KClO3), though the molecular mechanisms remain unclear. We assembled a high‐quality, 441.5 Mb genome of variety ‘Shixia’, with a contig N50 at 28.1 Mb, 29, 325 protein‐coding genes, 26 telomeres and 15 centromeres.
Guochun Zhao, Fan Jiang, Wenshun Hu, Jisen Zhang, Wen Wang, Siping Tu, Baiyu Wang, Qing Zhang, Jing Mei, Lianyu Lin, Yiying Qi, Xiuping Chen, Jianguo Li, Ray Ming, Shaoquan Zheng   +14 more
wiley   +1 more source

Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR). [PDF]

Nat Commun
Li W, Liu S, Fang X, Zou J, Jiang Q, Min X, Zhu X, Cao Y, Gao X, Han W, Azhar M, Xing X, Li F, Zhang Y, Liu H, Cheng L, Wang C, Bao J.   +17 more
europepmc   +1 more source

Genome Assembly Revealed MdZAT5 Coordinates Anthocyanin Biosynthesis in Apple Fruit Peel and Flesh by Interacting With MdHY5

Plant Biotechnology Journal, EarlyView.
ABSTRACT Anthocyanins are plant pigments that contribute to fruit coloration and nutritional quality, yet the coordinated regulation of their accumulation in both peel and flesh remains elusive. Here, we present a haplotype‐resolved genome of Malus cv. ‘Royalty’, a model cultivar with consistently red peel and flesh. A zinc‐finger transcription factor,
Mengnan Zhao, Mosen Zhang, Weilei Sun, Shiyao Duan, Jie Zhang, Tingting Song, Yaoxin Wang, Yuncong Yao, Ji Tian   +8 more
wiley   +1 more source

HDR

Cliopsy, 2015
openaire   +1 more source

Repurposing clinically safe drugs for DNA repair pathway choice in CRISPR genome editing and synthetic lethality. [PDF]

Nat Commun
Macak D, Kanis P, Riesenberg S.
europepmc   +1 more source