Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
"The Only People That Really Understand": A Qualitative Study of Healthcare Workers' COVID-19 Experiences and Implications for Workplace Support. [PDF]
Lee BEC +6 more
europepmc +1 more source
Counter-cultural and new age capitalist ideas: wellbeing and the contemporary workplace
PhDThis thesis examines the concept of wellbeing at work. It outlines a new type of organisation, that uses intellectual, immaterial and communicative labour power in order to provide a work ethic for both the production and consumption of their ...
Kunter, Aylin
core
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Factors Affecting the Mental Health and Wellbeing of Men in Nursing: A Systematic Review and Narrative Synthesis. [PDF]
Lim E, Zhang M, Higham K, Donnelly F.
europepmc +1 more source
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Standardising the preparation for, and delivery of care during, and after critical incidents: a Faculty of Sport and Exercise Medicine position statement. [PDF]
Heron N, Griffin S.
europepmc +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Infrastructure of tobacco and nicotine cessation services in Finnish healthcare: A cross-sectional study. [PDF]
Ylitörmänen T, Strand T, Ollila H.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source

