Results 51 to 60 of about 525,088 (306)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Predictors of cardio pulmonary resuscitation outcome in postoperative cardiac children

Journal of the Saudi Heart Association, 2016
Background: Outcomes of cardiopulmonary resuscitation (CPR) in children with congenital heart disease have improved and many children have survived after an in-hospital cardiac arrest.
Bana Agha Nasser, Julinar Idris, Abdu Rahman Mesned, Tageldein Mohamad, Mohamed S. Kabbani, Ali Alakfash   +5 more
doaj   +1 more source

Intercompartmental communication in senescence

FEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz, Eleanor J. Wind, Jixiang Leng, Myriam Gorospe   +3 more
wiley   +1 more source

Adrenergic Blockade Bi-directionally and Asymmetrically Alters Functional Brain-Heart Communication and Prolongs Electrical Activities of the Brain and Heart during Asphyxic Cardiac Arrest

Frontiers in Physiology, 2018
Sudden cardiac arrest is a leading cause of death in the United States. The neurophysiological mechanism underlying sudden death is not well understood.
Fangyun Tian, Tiecheng Liu, Gang Xu, Duan Li, Talha Ghazi, Trevor Shick, Azeem Sajjad, Michael M. Wang, Michael M. Wang, Michael M. Wang, Michael M. Wang, Michael M. Wang, Peter Farrehi, Peter Farrehi, Jimo Borjigin, Jimo Borjigin, Jimo Borjigin, Jimo Borjigin, Jimo Borjigin   +18 more
doaj   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

A comprehensive neuromonitoring approach in a large animal model of cardiac arrest

Animal Models and Experimental Medicine, 2022
Background Anoxic brain injuries represent the main determinant of poor outcome after cardiac arrest (CA). Large animal models have been described to investigate new treatments during CA and post‐resuscitation phase, but a detailed model that includes ...
Filippo Annoni, Lorenzo Peluso, Lucas Akira Hirai, Giovanni Babini, Amina Khaldi, Antoine Herpain, Lorenzo Pitisci, Lorenzo Ferlini, Bruno Garcia, Fabio Silvio Taccone, Jacques Creteur, Fuhong Su   +11 more
doaj   +1 more source

Wearable Device to Detect Cardiac Arrest [PDF]

, 2017
Cardiac arrest is one of the leading causes of death globally and is unwitnessed in most cases. This begs the question: how can anyone know that an individual is experiencing cardiac arrest if no one is around to see it?
Al Gumaei, Ashraf, Alashari, Majid, Dyke, Jon   +2 more
core   +2 more sources

Long-term deficits in cortical circuit function after asphyxial cardiac arrest and resuscitation in developing rats [PDF]

, 2017
Cardiac arrest is a common cause of global hypoxic-ischemic brain injury. Poor neurologic outcome among cardiac arrest survivors results not only from direct cellular injury but also from subsequent long-term dysfunction of neuronal circuits.
Clark, Robert S.B., Kochanek, Patrick M, Middleton, Jason W, Shoykhet, Michael, Simmons, Jennifer W, Simons, Daniel J   +5 more
core   +2 more sources

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source