Results 131 to 140 of about 189,544 (307)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Covering District Heating Demand with Waste Heat from Data Centres

open access: yesInternational Journal of Sustainable Energy Planning and Management
Currently, the huge potential of data centre waste heat for the decarbonization of the heating sector is often ignored. Here, a feasibility study is presented for covering the heat demand of two districts of Frankfurt (Germany) mainly by data centre ...
Bjarne Jürgens   +6 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Modelling the Size of Seasonal Thermal Storage in the Solar District Heating System

open access: yesMokslas: Lietuvos Ateitis, 2012
The integration of a thermal storage system into the solar heating system enables to increase the use of solar thermal energy in buildings and allows avoiding the mismatch between consumers’ demand and heat production in time.
Giedrė Streckienė   +1 more
doaj  

Changing demand: flexibility of energy practices in households with children (final report) [PDF]

open access: yes, 2015
This report summarises the findings and recommendations from the ‘Changing Demand: Flexibility of energy practices in households with children’ research project funded by the Consumer Advocacy Panel.
Yolande Strengers, Larissa Nicholls
core  

Integration of solar heating into heat recovery loops using constant and variable temperature storage

open access: yes, 2013
Solar is a renewable energy that can be used to provide process heat to industrial sites. Solar is extremely variable and to use it reliably thermal storage is necessary.
Walmsley, Michael R.W.   +3 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

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