Results 61 to 70 of about 3,177,040 (298)
FireDisentangling the combined effects of heat and ash in natural forest fires is challenging, hindering understanding of soil microbial post-fire responses.
Yu Sun +6 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
MetalsThis study comparatively investigates the coarse-grained heat-affected zone (CGHAZ) responses of two industrial X65 seamless pipeline steels with distinct composition–microstructure characteristics under simulated girth-welding thermal cycles.
Tianxiang Jiao +6 more
doaj +1 more source
Influence of Heat Inputs on Weld Profiles and Mechanical Properties of Carbon and Stainless Steel
Nigerian Journal of Technological Development, 2021 This study examines the effect of heat input on the weld bead profile, microstructure and mechanical properties of single V- joint welded carbon and stainless-steel plates.
L. O. Osoba +3 more
doaj
Metals, 2017 Various heat input conditions and post-weld heat treatments were adopted to investigate the microstructure evolution and mechanical properties of tungsten inert gas (TIG) welded joints of AA6082-T6 with porosity defects.
Bo Wang +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
The influence of return bends on the downstream pressure drop and condensation heat transfer in tubes [PDF]
, 1971 The influence of return bends on the downstream pressure drop and heat transfer coefficient of condensing refrigerant R-12 was studied experimentally. Flow patterns in glass return bends of 1/2 to 1 in. radius and 0.315 in. I. D.
Traviss, Donald P., Rohsenow, Warren M.
core
International Journal of Electrochemical Science, 2019 The susceptibility to corrosion of Inconel 718 weldments after double aging heat treatment was studied by the use of potentiodynamic tests. Plates of 3 mm thick were homogenized at 1080 °C during 1 hour and air cooled, then subjected to a solution ...
M.L. Hernández-Rodríguez +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source