Results 61 to 70 of about 231,992 (267)

Transient heat input model for friction stir welding using non-circular tool pin [PDF]

FME Transactions, 2020
Frictional heat source in the contact surface of a rotating tool and a stationary work piece is used to join metal pieces along the weldline in the friction stir welding process.
Leon Stephen J., Jayakumar V.
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Laser welding study for further development in essential power plant part repairs [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2015
The objective of this research work was to study the effects of laser welding when compared with shield metal arc welding (SMAW) process on the heat input, welded deposit rate, residual stress, distortion, microstructure and micro hardness.
Isarawit Chaopanich, Jitti Buapoon, Sirichai Wanavimolkul, Krisda Harnsombat, Supapol Oupanant, Sompong Tiasakul, Sakarin Bowngern, Tinnaphop Poolkhaolan   +7 more
doaj  

Gaz Tungsten Ark Kaynaklı 10 mm Kalınlığındaki AISI 316L Bağlantıların İçyapı ve Mekanik Karakterizasyonu

Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 2022
Östenitik paslanmaz çelikler, düşük sıcaklıklarda bile çok iyi şekillendirilebilirlik, iyi mekanik özellikler ve yüksek korozyon direnci gibi özelliklere sahiptir.
Gürel Çam, Hüseyin Tarık Serindağ
doaj  

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Development of Heat Input Estimation Technique for Simulation of Shell Forming by Line-Heating

Computer Modeling in Engineering & Sciences, 2007
A new hypothesis regarding heat transmission during line heating is proposed. It states that the distribution of the temperature of the gas adjacent to the plate, TG, and the overall local heat transfer coefficient, α, depend only on the distance from the torch. An identification technique for TG and α is developed.
Osawa, N., Hashimoto, K., Sawamura, J., Kikuchi, J., Deguchi, Y., Yamaura, T.   +5 more
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Recurrent Hypothermia and Autonomic Dysfunction Secondary to Shapiro Syndrome

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT A 44‐year‐old man presented with recurrent hypothermia, diaphoresis and hypertension. Extensive investigation for infectious, inflammatory, metabolic and endocrine aetiologies was negative. MR scan of the brain demonstrated no lesions but revealed callosal dysgenesis, consistent with Shapiro syndrome.
Naveen Kumar, Jeremy Johnson, Susanne Watkins, Eoin Mulroy   +3 more
wiley   +1 more source