Results 31 to 40 of about 1,211,483 (357)

IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design [PDF]

, 2017
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but ...
Angal, Bole, Borth, Breedveld, Chloe L. Stoyle, David P. Humphreys, Du, Durocher, Ellgaard, Feige, Feige, Freeze, Heath, Hendershot, Hendershot, Hu, Hubbard, Iwamoto, Katharine Cain, Leitzgen, Mains, Mason, Minegishi, Neil J. Bulleid, Nguyen, Paul E. Stephens, Peters, Sam Heywood, Schellenberger, Slanina, Spiess, Thies, Werther, Yang, Zou   +34 more
core   +1 more source

Clathrin heavy chain, light chain interactions. [PDF]

The EMBO Journal, 1983
Purified pig brain clathrin can be reversibly dissociated and separated into heavy chain trimers and light chains in the presence of non-denaturing concentrations of the chaotrope thiocyanate. The isolated heavy chain trimers reassemble into regular polygonal cage structures in the absence of light chains.
Fritz K. Winkler, K. Stanley
openaire   +3 more sources

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Kaluzab, Brigitte, Schwirzke, Marina, Weidle, Ulrich H., Weiss, Elisabeth H., Weissenhorn, Winfried   +4 more
core   +1 more source

Regulation of motor activity of ciliary outer-arm dynein by the light chain 1; Implications from the structure of the light chain bound to the microtubule-binding domain of the heavy chain

Biophysics and Physicobiology, 2023
Ciliary bending movements are powered by motor protein axonemal dyneins. They are largely classified into two groups, inner-arm dynein and outer-arm dynein. Outer-arm dynein, which is important for the elevation of ciliary beat frequency, has three heavy
Toshiki Yagi, Akiyuki Toda, Muneyoshi Ichikawa, Genji Kurisu   +3 more
doaj   +1 more source

Data to understand the biotransfer of heavy metals along the soil-plant-edible insect-human food chain in Africa

Data in Brief, 2023
Data on the biotransfer of heavy metals along the soil-plant-edible insect-human food chain collected along a 60km pollution gradient is presented here.
Susan Mwelwa, Donald Chungu, Frank Tailoka, Dennis Beesigamukama, Chrysantus M. Tanga   +4 more
doaj   +1 more source

Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription [PDF]

, 1991
Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to ...
Baltimore, David, Corcoran, Lynn M., Schlissel, Mark S.   +2 more
core   +5 more sources

Sequence-Based Discovery Demonstrates That Fixed Light Chain Human Transgenic Rats Produce a Diverse Repertoire of Antigen-Specific Antibodies

Frontiers in Immunology, 2018
We created a novel transgenic rat that expresses human antibodies comprising a diverse repertoire of heavy chains with a single common rearranged kappa light chain (IgKV3-15-JK1).
Katherine E. Harris, Shelley Force Aldred, Laura M. Davison, Heather Anne N. Ogana, Andrew Boudreau, Marianne Brüggemann, Michael Osborn, Biao Ma, Benjamin Buelow, Starlynn C. Clarke, Kevin H. Dang, Suhasini Iyer, Brett Jorgensen, Duy T. Pham, Payal P. Pratap, Udaya S. Rangaswamy, Ute Schellenberger, Wim C. van Schooten, Harshad S. Ugamraj, Omid Vafa, Roland Buelow, Nathan D. Trinklein   +21 more
doaj   +1 more source

Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain [PDF]

, 2008
The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest.
Kamiya Ritsu, King Stephen M., Liu Zhongmei, Nakazawa Yuki, Sakato Miho, Takazaki Hiroko, Yagi Toshiki, Yasunaga Takuo   +7 more
core   +2 more sources

Antibodies in a Heavy Chain Knock-In Mouse Exhibit Characteristics of Early Heavy Chain Rearrangement [PDF]

The Journal of Immunology, 2009
Abstract Studies in autoantibody transgenic mice have demonstrated receptor editing rearrangements at Ab H and L chain loci. However, the physiologic role of H chain editing (VH replacement and rearrangement on the second allele) has been called into question.
Wenzhao Meng, Eline T. Luning Prak, Robert A. Eisenberg, Lenka Yunk, Philip L. Cohen   +4 more
openaire   +2 more sources

AL amyloidosis with non-amyloid forming monoclonal immunoglobulin deposition; a case mimicking AHL amyloidosis

BMC Nephrology, 2018
Background Immunoglobulin heavy-and-light-chain amyloidosis (AHL amyloidosis) is a newly established disease entity where both the immunoglobulin heavy-chain and light-chain compose amyloid fibrils. The immunoglobulins responsible for the amyloid fibrils
Shun Manabe, Chihiro Iwasaki, Michiyasu Hatano, Fuyuki Kametani, Masahide Yazaki, Kosaku Nitta, Michio Nagata   +6 more
doaj   +1 more source