Results 51 to 60 of about 404,238 (256)

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

PHENOMENON OF LIGHT WATER IN PRODUCTS FOR ELDER PEOPLE

Вестник Северо-Кавказского федерального университета, 2022
Water belongs to the most important substance of all living organisms. Urbanization of society contributed to a significant increase in consumption of drinking and technical water and an increase in the amount of contaminated wastewater.
Mariya Gladkova, Gennady Kasyanov, Valentin Shipulin, Alexei Hristyuk   +3 more
doaj  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Study on Hydrogen Embrittlement Behavior in Heat-Affected Zone of X80 Welded Pipe

Metals
Hydrogen, as a clean energy source, has gradually become an important choice for the energy transformation in the world. Utilizing existing natural gas pipelines for hydrogen-blended transportation is one of the most economical and effective ways to ...
Lei Tang, Wang Liu, Bo-Chen Gao, Ji-Tong Sha, Ri-Xin Bai, Bai-Hui Che, Kai Xu, Gui-Ying Qiao, Fu-Ren Xiao   +8 more
doaj   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Hemoglobin synthesis rates in young trained and untrained males and females are not different

Physiological Reports
We employed deuterated water (2H2O) to compare the fractional synthetic rate (FSR) of hemoglobin in trained (n = 10) and untrained humans (n = 10). Participants had a mean V̇O2max of 49.8 [SD: 10.9] mL/kg/min, hemoglobin mass of 775 [180] g, and red ...
Hilkka Kontro, Chris McGlory, Martin J. MacInnis   +2 more
doaj   +1 more source

Heavy Water Fractionation during Transpiration [PDF]

Plant Physiology, 2007
A small proportion of water molecules contain the heavier isotopes of hydrogen and oxygen. There is a tendency for these heavier molecules of water to accumulate in leaves during transpiration.
Farquhar, Graham D., Cernusak, Lucas A., Barnes, Belinda   +2 more
openaire   +3 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source