Results 191 to 200 of about 194,330 (301)

ProTides for Antiviral Activity Beyond Liver Cells

Chemistry – A European Journal, EarlyView.
A strategy for obtaining prodrugs of antiviral nucleotides with broad tissue activity is presented that relies on cycloalkyl or cycloalkylalkyl esters, improving uptake and esterase cleavage, and producing nanomolar inhibitors in kidney, colon, and lung cells.
Felix Goebel, Adrian Humboldt, Heeyoung Kim, Carl J. M. Schölzel, Ralf Bartenschlager, Clemens Richert   +5 more
wiley   +1 more source

Expression of UCOE and HSP27 Molecular Elements to Improve the Stable Protein Production on HEK293 Cells. [PDF]

Biomed Res Int
Sosa-García C, Sánchez-Pacheco UA, Tavira-Montalvan CA, Meneses-Acosta A.   +3 more
europepmc   +1 more source

Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression. [PDF]

Commun Biol, 2023
Broche J, Köhler AR, Kühnel F, Osteresch B, Chandrasekaran TT, Adam S, Brockmeyer J, Jeltsch A.   +7 more
europepmc   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Phα1β interaction with the Kv11.1 potassium channel in HEK293 cells transfected with the human ERG channel. [PDF]

J Venom Anim Toxins Incl Trop Dis
Calixto JB, Dos Santos AA, Ferreira J, Souza AH, de Castro CJ, Gomez MV.   +5 more
europepmc   +1 more source

Expression of Large Full-Length PfEMP1 Proteins in HEK293 Cells. [PDF]

Methods Mol Biol, 2022
Renn JP, Doritchamou JYA, Duffy PE.
europepmc   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells. [PDF]

Life (Basel)
Fukunaga M, Watanabe S, Orihara K, Yamamoto N.   +3 more
europepmc   +1 more source

In Vitro Toxicity Evaluation of Cyanotoxins Cylindrospermopsin and Microcystin-LR on Human Kidney HEK293 Cells. [PDF]

Toxins (Basel), 2022
Diez-Quijada L, Puerto M, Gutiérrez-Praena D, Turkina MV, Campos A, Vasconcelos V, Cameán AM, Jos Á.   +7 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source