Results 181 to 190 of about 194,330 (301)

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

Stochastic model of IP3-induced Ca2+ spiking of HEK293 cells. [PDF]

PLoS Comput Biol
Azzoni C, Jüttner R, Sporbert A, Gotthardt M, Llewelyn Roderick H, Falcke M.   +5 more
europepmc   +1 more source

A Mechanistic Model of rAAV Production in Synthetic Cell Lines

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The recombinant adeno‐associated virus (rAAV) is a widely used vector for gene therapy. Its manufacturing faces significant challenges in producing the large quantities of vectors needed for clinical applications and reducing empty particles.
Prahalad Srinivasan, Han‐Jung Kuo, Yu‐Chieh Lin, Yen‐An Lu, Wei‐Shou Hu, Qi Zhang   +5 more
wiley   +1 more source

Diethylcarbamazine elicits calcium signals by activation of Brugia malayi TRP-2b channels heterologously expressed in HEK293 cells. [PDF]

Sci Rep
Williams PDE, Kashyap SS, Robertson AP, Martin RJ.   +3 more
europepmc   +1 more source

The cytosolic N-terminal region of heterologously-expressed transmembrane channel-like protein 1 (TMC1) can be cleaved in HEK293 cells. [PDF]

PLoS One, 2023
Yamaguchi S, Kamino M, Hamamura M, Otsuguro KI.   +3 more
europepmc   +1 more source

Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Interspecies cross‐reactive protein therapeutics that target conserved epitopes across species are critical for translational research. The present study showcases the engineering of an affibody molecule, originally discovered for binding to human death receptor 5 (hDR5) with 94 nM affinity, to simultaneously acquire cross‐reactivity to murine
Tse‐Han Kuo, Nagamani Vunnam, Jonathan N. Sachs, Benjamin J. Hackel   +3 more
wiley   +1 more source

Spectral Profiling of Early αsyn Aggregation in HEK293 Cells Modified to Stably Express Human WT and A53T-αsyn. [PDF]

Cells
Swaminathan P, Godø KS, Bjørn EB, Klingstedt T, Chatterjee D, Hammarström P, Nair RR, Lindgren M.   +7 more
europepmc   +1 more source

Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells. [PDF]

Int J Mol Sci, 2022
Wang YY, Hsu SH, Tsai HY, Cheng FY, Cheng MC.   +4 more
europepmc   +1 more source

Innovative methodology for flexible monitoring of various bioprocesses by using pre‐existing Raman data coupled with automated transfer learning technique

Biotechnology Progress, EarlyView.
The application of the transfer learning algorithm successfully allowed the use of a model built on process A for real‐time monitoring of process B. This study shows the improvement obtained with this methodology compared to the direct application of the model.
Adèle Schini, Hadi El Radi, Johan Cailletaud, Célia Sanchez, Nathan Gay, Bruno Ebel, Emmanuel Guedon, Laurent Jourdainne   +7 more
wiley   +1 more source

Kinetic analysis of endogenous β₂ -adrenoceptor-mediated cAMP GloSensor™ responses in HEK293 cells. [PDF]

Br J Pharmacol, 2023
Cullum SA, Veprintsev DB, Hill SJ.
europepmc   +1 more source