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2012
First discovered in the 1970s, DNA helicases were initially described as enzymes that use chemical energy to separate (i.e., to unwind) the complementary strands of DNA. Because helicases are ubiquitous, display a range of fascinating biochemical activities, and are involved in all aspects of DNA metabolism, defects in human helicases are linked to a ...
Maria Spies, Colin Wu
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First discovered in the 1970s, DNA helicases were initially described as enzymes that use chemical energy to separate (i.e., to unwind) the complementary strands of DNA. Because helicases are ubiquitous, display a range of fascinating biochemical activities, and are involved in all aspects of DNA metabolism, defects in human helicases are linked to a ...
Maria Spies, Colin Wu
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Helicase structure and mechanism
Current Opinion in Structural Biology, 2002Structural information on helicase proteins has expanded recently beyond the DNA helicases Rep and PcrA, and the hepatitis C virus RNA helicase to include UvrB, members of the DEA(D/H)-box RNA helicase family, examples of DnaB-related helicases and RuvB.
Jonathan M. Caruthers, David B. McKay
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Nature Structural & Molecular Biology, 2006
A new crystal structure reveals the path taken by single-stranded (ss) DNA through the central channel of a hexameric helicase. The path resembles a spiral staircase and provides an answer to the question of how hexameric helicases translocate on ssDNA.
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A new crystal structure reveals the path taken by single-stranded (ss) DNA through the central channel of a hexameric helicase. The path resembles a spiral staircase and provides an answer to the question of how hexameric helicases translocate on ssDNA.
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Nature Genetics, 2001
One of three loci previously associated with autosomal dominant progressive external ophthalmoplegia (adPEO) encodes ANT1, a mitochondrial nucleotide transporter. Now, mutations in two other genes are found in people with adPEO. One of these encodes a new helicase, Twinkle, which is related to the product of bacteriophage T7 gene 4, and co-localizes ...
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One of three loci previously associated with autosomal dominant progressive external ophthalmoplegia (adPEO) encodes ANT1, a mitochondrial nucleotide transporter. Now, mutations in two other genes are found in people with adPEO. One of these encodes a new helicase, Twinkle, which is related to the product of bacteriophage T7 gene 4, and co-localizes ...
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Enhancing helicase-dependent amplification by fusing the helicase with the DNA polymerase
Gene, 2008In this study, we have engineered a new bifunctional protein named "helimerase", by physically linking Thermoanaerobacter tengcongensis UvrD helicase (TteUvrD) and Bacillus stearothermophilus DNA polymerase I Large Fragment (Bstpol) using a coiled-coil.
Ying Li, Huimin Kong, Aurélie Motré
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Quarterly Reviews of Biophysics, 2003
1. Helicases as components of macromolecular machines 32. Helicases in replication 72.1 The loading of replicative helicases 72.1.1 Loading Rep helicase at the replication origin of bacteriophage ϕX174 72.1.2 How is a ssDNA strand passed through (and bound in?) the central channel of the hexameric replicative helicases? 82.1.3 Loading of E.
Emmanuelle Delagoutte +1 more
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1. Helicases as components of macromolecular machines 32. Helicases in replication 72.1 The loading of replicative helicases 72.1.1 Loading Rep helicase at the replication origin of bacteriophage ϕX174 72.1.2 How is a ssDNA strand passed through (and bound in?) the central channel of the hexameric replicative helicases? 82.1.3 Loading of E.
Emmanuelle Delagoutte +1 more
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Protein Displacement by Helicases
2009Helicases are ubiquitous enzymes that are vital to all living organisms. They are motor proteins that move in a specific direction along the nucleic acid and unwind the nucleic acid (DNA and RNA). ATP hydrolysis provides energy for helicase translocation and unwinding.
Laxmi Yeruva, Kevin D. Raney
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Quarterly Reviews of Biophysics, 2002
1. Mechanisms of nucleic acid (NA) unwinding by helicases 4322. Helicases may take advantage of ‘breathing’ fluctuations in dsNAs 4342.1 Stability and dynamics of dsNAs 4342.2 dsNAs ‘breathe’ in isolation 4352.3 Thermodynamics of terminal base pairs of dsNA 4382.4 Thermal fluctuations may be responsible for sequential base-pair opening at replication ...
Emmanuelle Delagoutte +1 more
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1. Mechanisms of nucleic acid (NA) unwinding by helicases 4322. Helicases may take advantage of ‘breathing’ fluctuations in dsNAs 4342.1 Stability and dynamics of dsNAs 4342.2 dsNAs ‘breathe’ in isolation 4352.3 Thermodynamics of terminal base pairs of dsNA 4382.4 Thermal fluctuations may be responsible for sequential base-pair opening at replication ...
Emmanuelle Delagoutte +1 more
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Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A)
2009Nuclear DNA helicase II (NDH II) was first isolated from calf thymus using a DNA-unwinding assay. Subsequently it has been shown to be a homologue of human RNA helicase A (RHA) and the maleless protein (MLE) from Drosophila. Accordingly, the protein possesses both DNA and RNA unwinding activities.
Frank Grosse, Suisheng Zhang
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Helicases at the Replication Fork
2012Helicases are fundamental components of all replication complexes since unwinding of the double-stranded template to generate single-stranded DNA is essential to direct DNA synthesis by polymerases. However, helicases are also required in many other steps of DNA replication. Replicative helicases not only unwind the template DNA but also play key roles
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